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Zhanfei Liu Rachel L. Sleighter Junyan Zhong Patrick G. Hatcher 《Estuarine, Coastal and Shelf Science》2011
How dissolved organic matter (DOM) undergoes chemical changes during its transit from river to ocean remains a challenge due to its complex structure. In this study, DOM along a river transect from black waters to marine waters is characterized using an offline combination of reversed-phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS), as well as tandem ESI-FTICR-MS. In addition, a water extract from degraded wood that mainly consists of lignins is used for comparison to the DOM from this transect. The HPLC chromatograms of all DOM samples and the wood extract show two major well-separated components; one is hydrophilic and the other is hydrophobic, based on their elution order from the C18 column. From the FTICR-MS analysis of the HPLC fractions, the hydrophilic components mainly contain low molecular weight compounds (less than 400 Da), while the hydrophobic fractions contain the vast majority of compounds of the bulk C18 extracted DOM. The wood extract and the DOM samples from the transect of black waters to coastal marine waters show strikingly similar HPLC chromatograms, and the FTICR-MS analysis further indicates that a large fraction of molecular formulas from these samples are the same, existing as lignin-like compounds. Tandem mass spectrometry experiments show that several representative molecules from the lignin-like compounds have similar functional group losses and fragmentation patterns, consistent with modified lignin structural entities in the wood extract and these DOM samples. Taken together, these data suggest that lignin-derived compounds may survive the transit from the river to the coastal ocean and can accumulate there because of their refractory nature. 相似文献
3.
Louise Schlüter Peter HenriksenTorkel Gissel Nielsen Hans H. Jakobsen 《Deep Sea Research Part I: Oceanographic Research Papers》2011,58(5):546-556
Phytoplankton composition and biomass was investigated across the southern Indian Ocean. Phytoplankton composition was determined from pigment analysis with subsequent calculations of group contributions to total chlorophyll a (Chl a) using CHEMTAX and, in addition, by examination in the microscope. The different plankton communities detected reflected the different water masses along a transect from Cape Town, South Africa, to Broome, Australia. The first station was influenced by the Agulhas Current with a very deep mixed surface layer. Based on pigment analysis this station was dominated by haptophytes, pelagophytes, cyanobacteria, and prasinophytes. Sub-Antarctic waters of the Southern Ocean were encountered at the next station, where new nutrients were intruded to the surface layer and the total Chl a concentration reached high concentrations of 1.7 ??g Chl a L−1 with increased proportions of diatoms and dinoflagellates. The third station was also influenced by Southern Ocean waters, but located in a transition area on the boundary to subtropical water. Prochlorophytes appeared in the samples and Chl a was low, i.e., 0.3 ??g L−1 in the surface with prevalence of haptophytes, pelagophytes, and cyanobacteria. The next two stations were located in the subtropical gyre with little mixing and general oligotrophic conditions where prochlorophytes, haptophytes and pelagophytes dominated. The last two stations were located in tropical waters influenced by down-welling of the Leeuwin Current and particularly prochlorophytes dominated at these two stations, but also pelagophytes, haptophytes and cyanobacteria were abundant. Haptophytes Type 6 (sensuZapata et al., 2004), most likely Emiliania huxleyi, and pelagophytes were the dominating eucaryotes in the southern Indian Ocean. Prochlorophytes dominated in the subtrophic and oligotrophic eastern Indian Ocean where Chl a was low, i.e., 0.043-0.086 ??g total Chl a L−1 in the surface, and up to 0.4 ??g Chl a L−1 at deep Chl a maximum. From the pigment analyses it was found that the dinoflagellates of unknown trophy enumerated in the microscope at the oligotrophic stations were possibly heterotrophic or mixotrophic. Presence of zeaxanthin containing heterotrophic bacteria may have increased the abundance of cyanobacteria determined by CHEMTAX. 相似文献
4.
Phytoplankton pigment patterns and community composition in the northern South China Sea during winter 总被引:1,自引:0,他引:1
Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the CHEMTAX software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.89±0.63 in the upper 50 m and 0.16±0.06 below 50 m depth. With increasing depth, APP decreased and photo-synthetically active radiation was attenuated. There was an obvious succession in the phytoplankton community from inshore to the open sea. Diatoms were dominant in the inshore region, while pelagophytes, Prochlorococcus, cyanobacteria and prymnesiophytes were dominant in the open sea. The vertical distribution of phytoplankton also differed greatly from inshore to the open sea. In the coastal and shelf region, diatoms were important components in the whole water column. Cyanobacteria also had a high abundance at the Subsurface Chlorophyll a Maxima (SCM) in the shelf region. In the slope and open sea, Prochlorococcus and cyanobacteria were important groups above the SCM, while pelagophytes dominated below the SCM. 相似文献
5.
We conducted studies of phytoplankton and hydrological variables in a semi-enclosed bay in northern China to understand the spatial–temporal variability and relationship between these variables. Samples were collected during seven cruises in Jiaozhou Bay from November 2003 to October 2004, and were analyzed for temperature, nutrients and phytoplankton pigments. Pigments from eight possible phytoplankton classes (Diatoms, Dinoflagellates, Chlorophyceae, Prasinophyceae, Chrysophyceae, Haptophyceae, Cryptophyceae and Caynophyceae) were detected in surface water by high performance liquid chromatography (HPLC). Phytoplankton pigment and nutrient concentrations in Jiaozhou Bay were spatially and temporally variable, and most of them were highest in the northern and eastern parts of the sampling regions in spring (May) and summer (August), close to areas of shellfish culturing, river estuaries, dense population and high industrialization, reflecting human activities. Chlorophyll a was recorded in all samples, with an annual mean concentration of 1.892 μg L−1, and fucoxanthin was the most abundant accessory pigment, with a mean concentration of 0.791 μg L−1. The highest concentrations of chlorophyll a (15.299 μg L−1) and fucoxanthin (9.417 μg L−1) were observed in May 2004 at the station close to the Qingdao Xiaogang Ferry, indicating a spring bloom of Diatoms in this area. Although chlorophyll a and other biomarker pigments showed significant correlations, none of them showed strong correlations with temperature and nutrients, suggesting an apparent de-coupling between the pigments and these hydrological variables. The nutrient composition and phytoplankton community composition of Jiaozhou Bay have changed significantly in the past several decades, reflecting the increasing nutrient concentrations and decline of phytoplankton cell abundance. The unchanged total chlorophyll a levels indicated that smaller species have filled the niche vacated by the larger species in Jiaozhou Bay, as revealed by our biomarker pigment analysis. 相似文献
6.
建立药用耐盐植物盐地碱蓬(Suaeda salsa)的HPLC指纹图谱。运用DAD-Agilent 1100高效液相色谱仪,色谱条件:Agilent Zorbax XDB C18柱(4.6 mm×250 mm;5.0μm)色谱柱,1.0 mL.min-1流速,320 nm检测波长,360 nm参比波长,10μL进样量,30℃柱温,乙腈(A)-0.1%磷酸水(B)梯度洗脱。建立了盐地碱蓬的HPLC指纹图谱,包含16个共有峰,指纹图谱特征明显,化学信息完整。方法的系统适应性测定结果符合指纹图谱的技术规范。结论:首次建立的盐地碱蓬HPLC参考指纹图谱可用于盐地碱蓬的快速真伪鉴别。 相似文献
7.
通过CCK8法检测分离自深海一株深海耐压菌Shewanella piezotolerans WP3次生代谢产物的细胞毒活性,研究了温度、压力、噬菌体对WP3次生代谢产物细胞毒活性的影响.发现WP3次生代谢产物具有良好细胞毒活性.低温因素可提高其细胞毒活性,在100μg/cm^3的含量下,代谢产物对肿瘤细胞Bel7402、CNE2、A549、HT1080细胞毒活性均有提高,其中对A549细胞毒活性提高了1.3倍.在最适温度(20℃)情况下,高压可降低其细胞毒活性.20 MPa培养条件下,代谢产物对4株肿瘤细胞均未表现出明显活性,而常压培养条件下对SW 480具有93.3%的抑制率,对HT1080抑制率表现出87.5%,对CNE2抑制率是79.8%.在低温情况下,压力因素对其细胞毒活性无影响,噬菌体对细胞毒活性影响不大.HPLC指纹图谱分析发现不同温度条件下,其次生代谢产物指纹图谱有变化,结合细胞毒活性结果分析,为后期分离鉴定细胞毒活性化合物提供指导. 相似文献
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9.
甲壳胺寡糖的液相色谱及薄层层析分析 总被引:1,自引:0,他引:1
为了解低分子甲壳胺多糖的抗肿瘤辅助疗效,作者利用高效液相色谱法(HPLC)结合薄层色谱法(TLC),探讨了SUGAR-KS-802寡糖分析柱分析甲壳胺寡糖的条件,并尝试了几种薄层层析展开剂对甲壳胺寡糖组分的展层条件。结果表明,在HPLC流动相为0.001mol/L NaOH溶液,流速0.2mL/min时,可将混合的标准甲壳胺寡糖成分很好地分离;采用乙酸乙脂:甲醇:水:氨水=5:9:1:1.5混合液作为硅胶薄板展开剂时,可将不同聚合度的甲壳胺寡糖很好地分开,且层析重现性高,糖残基数目和Rf值具有很好的线性关系。2种分析方法结合可以作为1种可靠的甲壳胺寡糖分析方法。 相似文献
10.
大气气溶胶中若干有机物的含量和季节变化 总被引:2,自引:2,他引:2
研究、探讨青岛近岸大气气溶胶中多芳烃类化合物的浓度水平、变化特征及影响因素。用高效液相色谱荧光检测法分析了青岛近岸大气气溶胶中的多环芳烃(PAHs)的含量水平,共检出13种多环芳烃化合物。结果表明该类化合物总量(∑PAHs)具有明显的季节特征,呈现春季<秋季<冬季的特征,人类活动可能是其主要来源。 相似文献