南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测          下载免费PDF全文

王飞  丛柏林  张朝晖  杨黄浩 《极地研究》2020,32(1):68-81

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。  相似文献   

Upper ocean high resolution regional modeling of the Arabian Sea and Bay of Bengal     

Suneet Dwivedi  Alok Kumar Mishr  Atul Srivastava 《海洋学报(英文版)》2019,38(5):32-50

In this paper, effort is made to demonstrate the quality of high-resolution regional ocean circulation model in realistically simulating the circulation and variability properties of the northern Indian Ocean(10°S–25°N,45°–100°E) covering the Arabian Sea(AS) and Bay of Bengal(BoB). The model run using the open boundary conditions is carried out at 10 km horizontal resolution and highest vertical resolution of 2 m in the upper ocean.The surface and sub-surface structure of hydrographic variables(temperature and salinity) and currents is compared against the observations during 1998–2014(17 years). In particular, the seasonal variability of the sea surface temperature, sea surface salinity, and surface currents over the model domain is studied. The highresolution model's ability in correct estimation of the spatio-temporal mixed layer depth(MLD) variability of the AS and BoB is also shown. The lowest MLD values are observed during spring(March-April-May) and highest during winter(December-January-February) seasons. The maximum MLD in the AS(BoB) during December to February reaches 150 m (67 m). On the other hand, the minimum MLD in these regions during March-April-May becomes as low as 11–12 m. The influence of wind stress, net heat flux and freshwater flux on the seasonal variability of the MLD is discussed. The physical processes controlling the seasonal cycle of sea surface temperature are investigated by carrying out mixed layer heat budget analysis. It is found that air-sea fluxes play a dominant role in the seasonal evolution of sea surface temperature of the northern Indian Ocean and the contribution of horizontal advection, vertical entrainment and diffusion processes is small. The upper ocean zonal and meridional volume transport across different sections in the AS and BoB is also computed. The seasonal variability of the transports is studied in the context of monsoonal currents.  相似文献   

南海东北部内潮的模态结构和传播     

刘倩  谢晓辉  尚晓东  陈桂英  汪宏 《海洋学报(英文版)》2019,38(9):12-23

The evolution of energy, energy flux and modal structure of the internal tides(ITs) in the northeastern South China Sea is examined using the measurements at two moorings along a cross-slope section from the deep continental slope to the shallow continental shelf. The energy of both diurnal and semidiurnal ITs clearly shows a～14-day spring-neap cycle, but their phases lag that of barotropic tides, indicating that ITs are not generated on the continental slope. Observations of internal tidal energy flux suggest that they may be generated at the Luzon Strait and propagate west-northwest to the continental slope in the northwestern SCS. Because the continental slope is critical-supercritical with respect to diurnal ITs, about 4.6 kJ/m~2 of the incident energy and 8.7 kW/m of energy flux of diurnal ITs are reduced from the continental slope to the continental shelf. In contrast, the semidiurnal internal tides enter the shelf because of the sub-critical topography with respect to semidiurnal ITs.From the continental slope to the shelf, the vertical structure of diurnal ITs shows significant variation, with dominant Mode 1 on the deep slope and dominant higher modes on the shelf. On the contrary, the vertical structure of the semidiurnal ITs is stable, with dominant Mode 1.  相似文献   

南海东北部东沙海域海水层反射特征分析     

李豪  关永贤  宋海斌  张锟  范文豪 《地球物理学进展》2020,(1):303-312

本文基于对南海东北部东沙海域近期采集的多道反射地震资料进行重新处理获得新的地震海洋学数据,分析了该海域内孤立波/内孤立波包、沙丘上方和陡坎附近特殊反射结构特征,从而提供了新的海水层与海底相互作用依据.研究结果表明,除之前已发表文章中地震海洋学资料显示存在的第一模态内孤立波/波包和沙丘上方常见的反射样式-披毛状发射外,地震海洋学资料上还发现了第二模态内孤立波、陡坎上方的上抬型波动反射结构样式.在新的地震海洋学数据中,第一模态内孤立波振幅均小于50 m,宽度上都小于5 km,单个内孤立波的最大振幅约为45 m.内孤立波包的内孤立波振幅都相对较小,均小于40 m,并且与之前不同的是,彼此之间振幅相差不大,没有明显的排列规律.此次地震海洋学数据记录到的第二模态内孤立波,形态较为完整,上层和下层反射的振幅相差不大,在30 m左右;中间层大约在水深130 m位置处,垂向结构的整体大小大于200 m.沙丘上方反射结构普遍存在弱反射层,可能是湍流边界层,并且存在特殊反射样式-披毛状反射.但并不是沙丘上方都存在披毛状反射样式,本文分析它出现在地震海洋学资料上可能是受测线与沙丘走向之间夹角的影响.陡坎区域的水层反射结构则表现为上抬型波动,并常常伴随着同相轴连续性的变化.该波动的大小及反射同相轴的连续性可能取决于陡坎的高度/坡度及水层动力的强度,新数据中出现的一个上抬型波动,高度达20~30 m,它的附近水层还存在一个形态不完整的内孤立波.陡坎附近的水层反射也常常出现弱反射带和小的波动.  相似文献   

基于IceBridge数据的南极别林斯高晋海的海冰厚度研究     

张建  杨元德  杨全明  汪楚涯 《冰川冻土》2020,42(2):295-306

海冰变化与全球气候、 生态系统和人类活动密切相关， 海冰厚度是海冰变化研究的重要参数之一。全面立体高精度观测海冰厚度的最有效手段是航空遥感， 而冰桥计划（IceBridge）是当前南北极最大的航空遥感工程。基于2009 - 2014年冰桥计划的激光雷达高程数据和数字测图系统相机光学影像对南极别林斯高晋海的海冰厚度进行研究， 并结合降雪量等气象数据探讨该区域海冰厚度变化的原因。研究发现该海域的海冰厚度在2009 - 2014年间整体呈微弱增长趋势（0.07 m·a^-1）， 但是在95%置信水平下不具有显著性。2009 - 2011年呈现先增加后减少的大幅度变化， 其中2010年达到极大值2.42 m， 之后开始缓慢增加。海冰厚度的年际变化与降雪和近地表温度等气象要素相关， 二者相比较而言降雪为主要影响因素。  相似文献   

Numerical Study on Tidal Mixing in the Bohai Sea     

Yifei Zhao  Ting Yu  Hu Wang 《Marine Geodesy》2019,42(1):46-63

The spatial and temporal variability of tidal mixing in Bohai Sea is studied using a numerical approach. In calculating tidal mixing, accurate barotropic tidal current is obtained via a harmonic analysis package utilizing the simulated current output from a high-resolution regional ocean model. And a “small-scale” roughness map is adopted to describe the detailed topographic features of Bohai Sea. It is shown that the tidal mixing estimated in Bohai Sea is much higher than the level of global background, and fluctuates considerably at some regions within a single day. In Liaodong Bay, Bohai Bay and Bohai Strait, the mixing varies greatly, with the peak value of O (10^?2) m² s^?1. The order of magnitude of mixing in Laizhou Bay is about O (10^?5～10^?3) m² s^?1. Mixing with background level of O (10^?5) m² s^?1 only appears in central area. Result also shows that rough topography plays relatively a more important role than tidal current in enhancing diapycnal mixing in Bohai Sea. The distributions of tidal mixing in selected sections reveal that the vertical stratification in Bohai Sea is not obvious, generally renders a barotropic structure.  相似文献   

东海陆架盆地南部中生代演化与动力学转换过程     

杨长清  杨传胜  孙晶  杨艳秋 《吉林大学学报(地球科学版)》2019,49(1):139-153

东海陆架盆地处于欧亚板块东南缘，其构造演化、动力学机制转换同太平洋板块与欧亚板块碰撞及印度-澳大利亚板块远程推挤效应有关。中生代以来，该盆地形成和演化过程受到古太平洋板块多期俯冲及多构造体系的叠加改造，地质构造和地球物理场复杂，盆地演化及动力学过程等一直是争论的焦点。本文利用最新调查资料，通过构造物理模拟实验、构造解析和平衡地质复原剖面等方法，结合区域构造背景，系统分析了东海陆架盆地中生代演化过程，探讨了其构造动力学转换过程。研究认为东海陆架盆地自中生代以来经历了晚三叠世前的被动大陆边缘和晚三叠世-中侏罗世活动大陆边缘挤压坳陷型盆地阶段，挤压应力来源于伊泽奈崎板块向欧亚大陆板块的低角度俯冲；早白垩世晚期-晚白垩世活动陆缘伸展断陷型盆地阶段，应力来源于太平洋板块向欧亚大陆板块俯冲后撤导致的岩石圈减薄作用；古近纪为弧后伸展断陷型盆地阶段。同时认为东海陆架盆地古特提斯构造域向古太平洋构造域转换的时间应该发生在中三叠世末期，古太平洋板块低角度俯冲和俯冲后撤代表华南中生代深部地质过程。  相似文献   

南海盐致环流对台风降水的响应特征分析     

刘娜  王桂华  巩远发  刘磊 《热带海洋学报》2019,38(3):13-21

本文采用多源卫星遥感数据通过统计分析的方法研究了17年间(2000—2016年)南海夏季(6—9月)台风对该海域降水、淡水通量的贡献及其可能导致的环流异常。主要结论如下: 1) 台风是南海中北部降水的重要影响因子, 可导致日平均降水量增加12mm, 约占南海夏季日平均降水(25mm·d ^-1)的一半, 且西北太平洋台风和南海“土台风”产生的降水分布存在显著的区域和强度差异; 2) 夏季, 南海由淡水通量引起的盐致环流表现为以海南岛东南部海域为中心的弱气旋式, 其流量量级约为-0.15Sv, 约占同期风生环流流量(约为-1.5Sv)的10%; 3) 夏季, 台风带来的降水使得南海中北部的气旋式盐致环流增强, 且西北太平洋台风降水导致的淡水通量变化引起的盐致环流强度要强于南海“土台风”。  相似文献   

Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

南海开放共享航次关键科学问题的思考——从多尺度海洋动力学角度出发     

杜岩  陈举  经志友  王祥鹏  陈更新  徐驰  储小青  陈植武  徐杰  施震  唐世林  何云开  梁韵  施平 《热带海洋学报》2020,39(6):1-17

南海是西太平洋最大的边缘海, 通过一系列的海峡与西太平洋和印度洋相联通, 其不同时空尺度的海洋环流动力过程及其生态环境效应是南海区域海洋学研究的重要内容。自20世纪50年代末全国第一次海洋普查开始, 我国对海洋调查的支持力度不断加大, 以科学考察船为代表的海洋科学观测平台建设不断加强; 进入新千年以来, 国内海洋科考船依托的各主要研究所和院校本着开放的理念, 先后组织多单位联合进行海上观测。尤其是最近10年, 国家自然科学基金委员会支持实施了船时共享航次计划, 进一步促进了国内海洋界的交流和合作, 南海区域海洋学的相关研究取得了很多重要的成果。从多尺度环流动力学的角度出发, 本文简要回顾了南海海洋观测的发展历程, 并初步总结了近些年来南海关键科学问题的研究进展, 包括南海和西太平洋的水体交换过程、南海中小尺度过程、多尺度相互作用及其生态环境效应等; 并且在现有的研究基础上, 对未来南海的观测和科学问题提出若干思考与展望。  相似文献