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海绵共附生微生物基因多态性的RAPD-PCR分析 总被引:1,自引:0,他引:1
采用传统分离手段对中国南海海域的细薄星芒海绵(Stelletta tenui(Lindgren,1897)1、皱皮软海绵(Halichondria rugosa(Ridley&Dendy))、澳大利亚厚皮海绵(Craniella australiensis(Carter))、贪婪倔海绵(Dysidea avara(Schmidt))4种海绵体内的微生物进行了分离培养,随机挑选64株芽孢杆菌属细菌(每种海绵16株)进行了RAPD-PCR基因多态性分析。研究表明,一些海绵微生物是可以通过传统分离手段得到的,来自同一或者不同海绵的微生物均具有丰富的基因多态性。 相似文献
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Applying randomly amplified polymorphic DNA (RAPD), the genetic variation of Cabomba caroliniana Gray (cabomba or fanwort), a new alien plant in China, was analyzed in this paper. Total 143 bands, including 47 polymorphic bands, were amplified from 23 primers in 20 samples. The sampling distance was large, but its genetic diversity was low. The main results were that: (1) Cabomba, which grew and dispersed mainly in fragment, was an abundant and dominant species in freshwater, and its main dispersal mechanism was vegetative reproduction. (2) Cabomba was originally introduced into China as an aquarium submerged plant. Somehow, those discarded cabomba became invasive species in the areas of Hangzhou, Shanghai, and Meicheng, and other places. (3) Although the level of genetic diversity in cabomba was low, their rapid dispersion and propagation could seriously harm to local aquatic community. Therefore, specific measure should be used to control cabomba from uncontrolled spreading and damage to local vegetation communities. 相似文献
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Applying randomly amplified polymorphic DNA (RAPD), the genetic variation ofCabomba caroliniana Gray (cabomba or fanwort), a new alien plant in China, was analyzed in this paper. Total 143 bands, including 47 polymorphic
bands, were amplified from 23 primers in 20 samples. The sampling distance was large, but its genetic diversity was low. The
main results were that: (1) Cabomba, which grew and dispersed mainly in fragment, was an abundant and dominant species in
freshwater, and its main dispersal mechanism was vegetative reproduction (2) Cabomba was originally introduced into China
as an aquarium submerged plant. Somehow, those discarded cabomba became invasive species in the areas of Hangzhou, Shanghai,
and Meicheng, and other places. (3) Although the level of genetic diversity in cabomba was low, their rapid dispersion and
propagation could seriously harm to local aquatic community. Therefore, specific measure should be used to control cabomba
from uncontrolled spreading and damage to local vegetation communities.
Supported by National Natural Science Foundation of China (No. 39970145) and Major State Basic Research Development Program
of China (No. G2000046803). 相似文献
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