饲料中添加芽孢杆菌组分对凡纳滨对虾幼虾生长、能量代谢和抗WSSV能力的影响     

单洪伟  于鹏  刘宽  于明超  马甡 《中国海洋大学学报(自然科学版)》2020,(1):30-38

以凡纳滨对虾(Litopnnaus vannamei)幼虾为实验动物,在其基础饲料中分别添加芽孢杆菌(Bacillus sp.)胞外蛋白粗提物(1.0%,胞外蛋白组)、胞内多糖粗提物(1.0%,胞内多糖组),以基础饲料为对照组饲料,进行为期28d的养殖实验,探讨芽孢杆菌胞外蛋白和胞内多糖粗提物对幼虾生长、能量代谢及抗病能力的影响。研究表明,芽孢杆菌2种粗提物对凡纳滨对虾的生长和成活率影响不显著(P>0.05)。饲喂2种粗提物影响了凡纳滨对虾肝胰腺和肌肉中能量代谢相关酶活力。胞外蛋白组肝胰腺中磷酸果糖激酶(PFK)、乳酸脱氢酶(LDH)活力在养殖实验前期显著高于对照组(P<0.05),而肌肉中PFK、LDH活力显著低于对照组(P<0.05);胞内多糖组肝胰腺中己糖激酶(HK)活力及肌肉中PFK、LDH活力显著低于对照组(P<0.05)。饲喂2种粗提物均显著提高了凡纳滨对虾肌肉中琥珀酸脱氢酶(SDH)活力和肝胰腺中电子传递系统(ETS)活力(P<0.05),胞外蛋白粗提物对肝胰腺中的SDH活力也有显著的提高作用(P<0.05)。饲喂2种粗提物显著降低了凡纳滨对虾肝胰腺及肌肉组织中脂肪酸合酶(FAS)含量(P<0.05)。此外,饲喂2种粗提物均会显著提高凡纳滨对虾肠道蛋白酶活力(P<0.05),而胞外蛋白粗提物使凡纳滨对虾肠道脂肪酶活力显著降低(P<0.05)。白斑综合征病毒(WSSV)感染后,与对照组相比,胞外蛋白组和胞内多糖组凡纳滨对虾半致死时间分别延长了26.19%和7.14%。根据以上结果推测,芽苞杆菌2种粗提物能够影响凡纳滨对虾能量代谢水平并提高其抗WSSV能力,且二者之间密切相关。  相似文献   

菲律宾蛤仔肉不同提取物呈味特性     

饶梦微  章超桦  林海生  曹文红  秦小明  郑惠娜  高加龙 《广东海洋大学学报》2022,(1)

【目的】系统评价菲律宾蛤仔(Ruditapes philippinarum)肉不同提取物的呈味物质含量和呈味特征差异。【方法】以新鲜菲律宾蛤仔肉为原材料,采用水煮和酶解的不同提取方式,制得两种冻干粉,比较分析两者游离氨基酸、呈味核苷酸、有机酸、有机碱和无机离子含量的差异,通过滋味活性值(Taste Activity Value,TAV)评价其对滋味的贡献,并结合感官评价法和电子舌检测法考察其整体呈味特征。【结果】感官评定结果表明,水煮提取物鲜味和甜味强于酶解提取物;鲜味、甜味和苦味是酶解提取物的重要味觉特征;电子舌主成分分析结果亦表明水煮提取物更接近谷氨酸钠的呈鲜特性。水煮和酶解提取物中游离氨基酸、呈味核苷酸、有机酸、有机碱和无机离子的含量均存在显著差异(P<0.05)。不同的呈味物质由于含量的差异及呈味组分间的相互作用导致水煮和酶解提取物的整体滋味存在差异。【结论】菲律宾蛤仔的呈味特性与其水溶性呈味物质密切相关,酶解提取物虽增加了氨基酸等的总体含量,但对鲜味、甜味的贡献不大。  相似文献   

采卤对接井施工工艺的工程实践          下载免费PDF全文

谷惠东 《探矿工程》2021,48(S1):154-159

以江西九二盐业403-404采卤对接井组的施工工程为案例,系统介绍了井区地质情况、钻探施工情况;总结了大口径取心工具的优点,螺杆定向钻进施工工艺及攻克技术难点。  相似文献   

基于高分六号WFV数据的冬小麦种植面积提取     

刘宝康  王仁军  尤晓妮  于志远  南岭  张起升  黄炳婷 《测绘与空间地理信息》2021,44(1):1-4

冬小麦是我国重要的粮食作物之一,准确获取冬小麦种植面积具有重要的现实意义。为探究高分六号卫星影像进行冬小麦遥感监测的可行性和精确性,本文选取甘肃省崆峒区为研究区,运用红边波段+监督分类中的支持向量机法,提取了2019年崆峒区冬小麦种植面积,并利用混淆矩阵对分类结果进行精度验证。结果表明:提取崆峒区冬小麦种植面积为15045 hm 2,与实际种植面积相比,误差率为1.02%;该模型能有效地提取崆峒区冬小麦,总体精度为98.88%,Kappa系数为0.97;红边波段能有效地提取干扰地物,提取精度比直接使用监督分类高7.88个百分点;GF6影像在提取冬小麦种植面积上具有明显优势。  相似文献   

Possible change in distribution of seaweed, Sargassum horneri, in northeast Asia under A2 scenario of global warming and consequent effect on some fish     

Teruhisa Komatsu  Masahiro Fukuda  Atsuko Mikami  Shizuha Mizuno  Attachai Kantachumpoo  Hideaki Tanoue  Michio Kawamiya 《Marine pollution bulletin》2014

Global warming effects on seaweed beds are already perceptible. Their geographical distributions greatly depend on water temperatures. To predict future geographical distributions of brown alga, Sargassum horneri, forming large beds in the northwestern Pacific, we referred to future monthly surface water temperatures at about 1.1° of longitude and 0.6° of latitude in February and August in 2050 and 2100 simulated by 12 organizations under an A2 scenario of global warming. The southern limit of S. horneri distribution is expected to keep moving northward such that it may broadly disappear from Honshu Island, the Chinese coast, and Korean Peninsula in 2100, when tropical Sargassum species such as Sargassum tenuifolium may not completely replace S. horneri. Thus, their forests in 2100 do not substitute those of S. horneri in 2000. Fishes using the beds and seaweed rafts consisting of S. horneri in East China Sea suffer these disappearances.  相似文献   

基于DEM的莱州湾南岸典型水系河间地提取及区域地貌演化研究     

杜国云 《第四纪研究》2015,35(2)

以SRTM3-DEM为数据源,在ArcGIS支持下,采用水文分析以及DEM分析数据与地质图、卫星图像和地形图区域数据综合对比分析,结合实地调查及前人研究成果,对莱州湾南岸的弥河、白浪河、潍河3条典型河流河网河间地的提取进行了研究,并对不同流域河间地比率的空间关系、流域地貌侵蚀对比与区域变化、全新世河型及河型转换以及区域地貌发展阶段进行了研究.通过DEM的水文分析、河网提取、河间地制图,得到了每条河流、每种累积流量取值下河网中的河间地,从而提出了快速提取河间地的一种新方法.区域空间上,河间地比率自西向东存在弱的增大变化.流域侵蚀综合分析表明:3条河流曾明显下切,即西部的弥河以单河道截切式下切;中部的白浪河以拓展式下切;东部的潍河呈嵌入式下切.表明西部侵蚀下切幅度大于东部(西部河流侵蚀速度快于东部),从而印证了河间地比率西小、东大的空间变化.对古河型(/河网)分析表明,全新世以来有网状河、扇状分汉河、汇聚型河、顺直河和曲流河5种河型.河型转换受控于气候变化、基准面下降和不均衡的构造抬升.将研究区全新世的河流地貌演化分为全新世早阶段、全新世中期早阶段、全新世中期晚阶段、全新世晚期早阶段和全新世晚期晚阶段5个阶段.  相似文献   

基于 DEM 对喀斯特地貌山地及水系信息的提取--以桂林阳朔县为例   总被引：1，自引：0，他引：1  

李飞  杨小平  郝红科 《测绘与空间地理信息》2015,(8):73-76

以30 m分辨率的SRTM－DEM数据为基础,通过GIS的空间分析功能,首先对数据进行坡度分析,通过坡度分级法将山地与平原划分,自动提取出山地的信息,再运用碎斑处理结合TM影像对结果进行修正;其次,在ArcGIS的水文分析（ Hydrology）模块下对 DEM进行数字地形分析,提取流域特征。经分析得到了研究区域的山地及河网特征信息,并与实际地形及河流水系大致吻合,改进了传统手工和野外调查为主的提取方法,初步实现了山地、水系信息的自动提取,为研究区域社会经济更好更快地发展具有一定的参考意义。  相似文献   

发光细菌法在环渤海污水样品遗传毒性检测中的应用     

崔志松  栾晓  高伟  李倩  杨官品  郑立 《海洋与湖沼》2015,46(1):228-232

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。  相似文献   

Recurrence of the world’s largest green-tide in 2009 in Yellow Sea, China: Porphyra yezoensis aquaculture rafts confirmed as nursery for macroalgal blooms     

Dongyan Liu  John K. Keesing  Zhijun Dong  Baoping Di  Peter Fearns 《Marine pollution bulletin》2010,60(9):1423-1432

In the summer of 2008, the world’s largest green-tide occurred in the Yellow Sea, China. The hypothesized cause was the expansion of Porphyra yezoensis aquaculture along the Jiangsu coastline and the re-occurrence of a green-tide in 2009 was predicted. In this study, satellite and field images showed the formation of the June 2009 green-tide which again originated from the Jiangsu coast. The responsible species, its source and biomass accumulation were studied to support the previous hypothesis. Morphological and phylogenetic analysis demonstrated the homology of Ulva prolifera in the 2008 green-tide with the U. prolifera from P. yezoensis aquaculture rafts. About 91-505 kg/ha of U. prolifera was attached to the P. yezoensis aquaculture rafts and a total biomass of 4956 tonnes was estimated during the harvesting of P. yezoensis. This is sufficient to seed a bloom when they are dislodged from the rafts as a result of harvesting practices.  相似文献   

Evaluation ofAntitumor, Immunomodulatory and Free Radical Scavenging Effects of A New Herbal Prescription Seaweed Complex Preparation     

LIU Xin  SHAO Changlun  KONG Wenwen  FANG Yuchun  WANG Changyun 《青岛海洋大学学报(英文版)》2013,(3):515-520

Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.  相似文献