不同盐度对文蛤(Meretrix meretrix)呼吸代谢及体内酶活性的影响     

白奕天  丁天扬  李东东  聂鸿涛  闫喜武 《海洋科学》2018,42(6):123-131

为研究不同盐度对文蛤呼吸代谢的影响,本实验设置5个盐度(‰)梯度(11、18、25、32、39),检测不同盐度对文蛤(Meretrixmeretrix)耗氧和排氨的影响,以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na+/K+-ATP酶活性的变化。结果表明:随着盐度的不断升高,文蛤耗氧率先升后降再升,在盐度18时达到最大值;排氨率先升后降,在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长,文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P0.05),酶活力在盐度39时为最高;随着盐度不断升高和胁迫时间延长,文蛤的外套膜中Na+/K+-ATP酶活力总体呈先下降再升高后下降的趋势(P0.05),在盐度32时为最高;文蛤的外套膜和鳃中乳酸脱氢酶活力以及鳃和肝胰腺中Na+/K+-ATP酶活力受盐度影响不显著(P0.05),酶活力变化也多呈现"W"形的变化趋势。研究结果为文蛤的人工养殖提供参考。  相似文献   

酒依赖致慢性酒精中毒性脑病的临床及CT/MRI分析     

余红胜  武宁强  沈又利 《CT理论与应用研究》2010,19(3):107-116

目的:分析酒依赖致慢性酒精中毒性脑病(CAE)的临床及CT/MRI影像表现特征,探讨CT/MRI在该病临床诊断中的价值。方法:回顾性分析32例资料完整CAE患者的临床及CT/MRI影像学资料,总结其影像学表现特征。结果:CAE临床表现主要为不同程度的神经系统损害(尤为中枢神经系统)和精神障碍,其颅脑损害CT/MRI主要表现为:脑萎缩27例;脑梗塞7例;白质脱髓鞘18例;韦尼克脑病3例;胼胝体变性1例;多病变并存24例。结论:①CAE的病理机制为饮酒导致的脑血管损害和营养代谢障碍,以及脑组织缺血缺氧、神经纤维脱髓鞘改变;②CAE影像学表现有其特征性,尤以小脑蚓部萎缩以及韦尼克脑病、胼胝体变性更具特征性,CT/MRI检查对了解脑损害的性质、范围及程度有重要意义,可为临床诊断、治疗及评估预后提供依据。  相似文献   

南海卡氏豆荚软珊瑚化学成分研究Ⅱ     

万一千  刘青 《热带海洋学报》1997,(3)

运用光谱分析和GC－MS方法首次从卡氏豆荚软珊瑚Lobophytumcatelai中分离和鉴定出8个化合物：l，十六碳酸；2,异丁基十六碳酸酯；3，鲨肝醇；4十六酸丙酯；5，2，3一二羟基丙基十八酸酯；6，2β，3β-环氧基-5α-雄甾烷-17-醇；7，14-甲基-5α-胆甾-8-稀-3-酮；8，2α，3α一环氧基-5α-雄甾烷-17-醇。  相似文献   

Facile Conversion of Alcohols to Olefins by Tosylation and Subsequent SiO2- promoted β-elimination     

RUAN Xiaohong  SONG Gaopeng  ZHANG Yichun  LI Yingxia 《中国海洋大学学报(英文版)》2007,6(2)

A convenient and effective procedure was developed for the conversion of alcohol to olefin by tosylation and subsequent β-elimination promoted by silica gel in this study. Treatment of the alcohols with p-toluenesulfonyl chloride in pyridine at 0℃ affords tosylates which undergo β-elimination with silica gel in dichloromethane or chloroform at room temperature, yielding olefinswith high productivity.  相似文献   

Cloning and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase Encoding Gene in Gracilaria/Gracilariopsis lemaneiformis     

REN Xueying SUI Zhenghong ZHANG Xuecheng 《中国海洋大学学报(英文版)》2006,5(2):146-150

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.  相似文献   

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Non-equilibrium alcohol flooding model for immiscible phase remediation: 1. Equation development     

Stanley Reitsma  Bernard H. Kueper 《Advances in water resources》1998,21(8):1

The equations for a compositional model for simulation of a two-phase, three-component system with inter-phase mass transfer are developed. Emphasis is placed on development of inter-phase mass transfer equations for incorporation of rate-limited inter-phase mass transfer. Due to the nature of the three-component systems considered, a single-film model may be inadequate and a two-film model must be utilized. A two-film model accounts for the simultaneous transfer of components in both directions across phase interfaces. The effect of interaction between components on diffusion is considered using a general form of Fick's Law. A Hand Plot representation of ternary phase behavior is chosen since it allows for straightforward calculation of miscibility of bulk phases under conditions of local non-equilibrium. The developed set of equations form the basis for a numerical model to simulate the enhanced removal of non-aqueous phase liquids (NAPLs) from porous media using single-component alcohol floods.  相似文献   

Molecular Cloning of a Cytosolic G6PDH Gene from Populus Suaveolens and Its Expression to Improve the Cold Resistance of Tobacco Plants     

LIN Yuan-zhen  GUO Hai  ZHANG Zhi-yi  LIN Shan-zhi 《冰川冻土》2009,31(6)

Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) is the first and main regulated enzyme of oxidative pentose phosphate pathway (OPPP), catalyzing the conversion of glucose-6-phosphate to 6-phospho-gluconolactone and playing important roles in the growth and development of plants. It is preciously reported that the enhancement of freezing resistance of Populus suaveolens cuttings is clear related to the distinct increase in cytosolic G6PDH activity. Here, a 1697 bp cDNA fragment (PsG6PDH) is amplified by RT-PCR from cold-induced total RNA of the freezing-tolerant P. suaveolens . A sequence analysis showed that PsG6PDH coding region had 1 530 bp and encoded 510 predicted amino acid residues. Genomic Southern analysis revealed that the isoform is encoded by a few copies of the gene in the poplar genome. The cloned gene PsG6PDH is cloned into binary vector pBI121 and used to transform tobacco. PCR and Southern blotting results verified integration of this gene into the genome of tobacco. Moreover, cold treatment experiments and membrane defense enzyme activity analysis confirmed that overexpression of the PsG6PDH gene could enhance the tolerance to cold or frigid stresses in transgenic plants.  相似文献   

Ice- and cryogel-soil composites in water-retaining elements in embankment dams constructed in cold regions     

N. K. Vasiliev  A. A. Ivanov  I. N. Shatalin  V. V. Sokurov 《寒旱区科学》2013,5(4):0444-0450

There are a number of serious problems connected with building and repairing water-retaining elements in embankment dams in cold regions. One of them is the difficulty in finding inexpensive clay materials with the necessary structural properties and using them in the winter. Another is the cracks that appear in the upper part of a dam when the core freezes to the banks, and leakage along the cracks threatens to destroy the dam. Still another is the process of erosion at the bottom of the core which may occur through fissures in the rock foundation of a dam and in transport constructions. Also, the behaviour of water-retaining elements during earthquakes is unreliable. All of these problems can be solved by using ice- and cryogel-soil composites created by cryotropic gel formation (CGF). Our laboratory investigations demonstrate that the materials proposed for water-retaining elements have the necessary permeable, plastic, thermophysical, and strength properties to solve all of these problems. Certain constructions of water-retaining elements which may prove to be both safe and cost-effective are proposed. However, these ice- and cryogel-soil composites need to be field-validated before they are used in dams and transport structures in cold regions.  相似文献   

硅酸盐聚乙稀醇冲洗液在新疆砂岩性铀矿钻探中的应用          下载免费PDF全文

刘维鹏  许青海  白宝云 《探矿工程》2012,39(7):21-23,27

分析并研究了硅酸盐聚合物冲洗液体系的作用机理,配制了一套专门针对砂岩性铀矿岩心钻探中复杂地层孔段长、极易引发多种孔内事故的冲洗液体系。生产实践证明,该冲洗液体系完全能够满足绳索取心钻进工艺各项性能要求,并在对付砂岩性复杂地层方面具有良好的效果。  相似文献