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在三亚海域设置3个站位, 分别于各站位连续采集12个月表层水样。利用流式细胞仪进行浮游病毒及浮游细菌丰度的测定, 并对两者及其与环境因子之间的相关性进行研究, 同时对不同站位之间进行差异性分析。结果表明, 调查海域浮游病毒丰度(平均7.63×106viruses·mL-1)高于浮游细菌丰度(平均1.52×106cells·mL-1)。浮游病毒及浮游细菌丰度在三亚河口最高, 且有明显的季节变化, 冬春季高于夏秋季, 并且其与鹿回头半岛西侧、小东海之间均有极显著差异(P<0.01), 不同类群病毒代表的宿主类群也有所不同。调查海域总体水平浮游病毒丰度与浮游细菌丰度显著正相关(r=0.800, P<0.01); 叶绿素(Chl a)和氮盐(NO- 2、NO- 2+NO- 3、NH+ 4)是影响两者的关键因子。 相似文献
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Uncovering the role of environmental factors and finding critical factors which harbor significant fractions in governing microbial communities remain key questions in coastal marine systems. To detect the interactions between environmental factors and distributions of virio-and bacterioplankton in trophic coastal areas, we used flow cytometry to investigate the abundance of virio-and bacterioplankton covering 31 stations in the Bohai Sea of China. Our results suggested that the average abundance of total virus(TV) in winter(～2.29×10~8 particles/mL)was slightly lower than in summer(～3.83×10~8 particles/mL). The mean total bacterial abundance(TB) was much lower in winter(～2.54×107 particles/mL) than in summer(～5.43×10~7 particles/mL). Correlation analysis via redundancy analysis(RDA) and network analysis among virioplankton, bacterioplankton and environmental factors revealed that the abundances of viral and bacterial subpopulations depend on environmental factors. In winter, only temperature significantly influenced the abundances of virio-and bacterioplankton. In summer, in addition to temperature, both salinity and nutrient(SiO_2) had a remarkable impact on the distribution of virioand bacterioplankton. Our results showed a clear seasonal and trophic pattern throughout the whole water system, which revealed that temperature and eutrophication may play crucial roles in microbial distribution pattern. 相似文献
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A better understanding of bacterioplankton community shifts following change in marine environments is critical to predict the marine ecosystem function. In order to get a snapshot of the microbial taxonomy profiling of a wide range marine area, a quick, convenient and low cost method would be favorable. In this study, we developed a 16S rRNA gene-based microarray using ARB software, which contained 447 probes targeting 160 families of marine bacteria. The specificity, sensitivity and quantitative capability of this microarray were assessed by single cloned16S rRNA genes. The reliability of this microarray was tested by eight environmental samples. The results showed that the microarray was specific, only 1.16% false results were detected in five single-clone hybridization tests. The microarray could detect DNA samples as few as 1 ng/μL and the signal intensity could reflect the relative abundance of the bacteria in the range of 1 ng/μL to 100 ng/μL of DNA concentration. Hybridization with environmental samples showed that it can discriminate bacterioplankton communities by sites and time. High throughput sequencing results from the eight samples confirmed the hybridization results. It indicated that this developed microarray could be used as a convenient tool to monitor the bacterioplankton community in marine environment. 相似文献
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Bacterioplankton productivity (BP) spatial variation was investigated in relation to potential resources, including primary productivity and dissolved organic matter, in the micro-tidal Neuse River–Pamlico Sound estuarine system, North Carolina, USA. Estuarine BP was predicted to correlate with the trophic gradient, decreasing along the salinity gradient in parallel with the decrease in organic matter and primary productivity. This prediction was tested over four years at spatial scales ranging from kilometers to meters along the riverine axis and with depth. The general pattern of BP across the salinity gradient was unimodal and matched the phytoplankton patterns in peak location and variability. Peak locations varied with discharge, especially in 2003 when above average discharge moved peaks downstream. Spatial coherence of BP with other variables was much less at short time scales. The effect of temperature, nutrients, and phytoplankton on BP varied by location, especially fresh versus brackish stations, although only temperature explained more than 20% of the BP variation. Depth variation of BP was as great as longitudinal variation and bottom samples were often higher than surface. BP was strongly correlated with particulate organic carbon at the pycnocline and bottom, highlighting the importance of particulate matter as a resource. Station-averaged BP and phytoplankton data corresponded well with two published meta-analyses, although the offset of the freshwater station suggested longitudinal differences in community composition or resource availability. 相似文献
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2012年夏季中国第5次北极科学考察期间,对北冰洋楚科奇海及其北部边缘海浮游细菌丰度和生产力进行了测定,并将其与环境因子进行了相关性分析。结果显示,楚科奇海浮游细菌丰度的变化范围为0.56×108~6.41×108 cells/dm3,平均为2.25×108 cells/dm3;细菌生产力介于0.042~1.92mg/(m3·d)(以碳计)之间,平均为0.54mg/(m3·d)(以碳计),与已有研究结果基本相当。陆架区细菌丰度和生产力要明显高于北部边缘区,但前者的单位细菌生产力则较低。与环境因子的相关性分析显示,细菌丰度与温度和叶绿素a浓度存在显著正相关(p0.01),表明北极变暖导致的海水升温及浮游植物生物量的增加均会促进细菌的生长,从而进一步提高细菌在海洋生态系统和碳循环中的作用。但陆架区的细菌生产力与环境参数均没有显著相关性,表明其影响因素较为复杂;生产力在北部边缘区则仅与叶绿素a存在显著正相关(p0.01),表明浮游植物生长过程产生的溶解有机碳(DOC)是细菌生长最为主要的碳源,碳源的单一可能制约细菌的生产从而导致该海域无冰状态下细菌丰度的增加不如预期,但融冰过程带来的大量DOC将促进细菌活性的增加。 相似文献
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春秋季山东南部近岸浮游细菌生态分布 总被引：1，自引：0，他引：1
分别于2007年4月(春季)和10月(秋季),对山东南部近岸海域进行了现场调查,研究了该海区浮游细菌丰度、生物量分布特征,探讨了它们与温度、溶解氧(DO)、总氮(N)、总磷(P)、硝酸盐(NO-3)、铵盐(NH+2)及活性磷酸盐(PO4-P)的相关性.结果表明:浮游细菌生物量具有一定的时间、空间分布特征,春季浮游细菌丰度及生物量要高于秋季,2个季节近岸细菌数量高于远岸区域;浮游细菌丰度及生物量与温度、DO、总P、NO-3、NH+2及PO4-P均呈显著相关关系(P<0.01),表明上述因子可能是该海域浮游细菌数量分布的主要限制因子. 相似文献
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Distribution, variation and impact factors of biomass of bacterioplankton from April to May 1999 in Bohai Sea were studied in DAPI method with epifluorescence microscopy. The biomass in surface waters showed a small day-night variation, varying from 0.13 to 2.51μg/dm^3 with an average of 0.84μg/dm^3. The biomass in bottom waters showed, however, a large variation, changing from 0.15 to 4.18μg/dm^3 with an average of 1.36μg/dm^3. The peak values occurred at 5 and 11 a.m. The bottom water biomass showed a significant correlation with particulate organic carbon （r=0.639, P〈0.05）. Heterotrophic bacterioplankton biomass was high in nearshore waters and low in offshore areas with a high biomass zone around Huanghe （Yellow） River mouth, showing the same distribution of nutrients. In vertical distribution, heterotrophic bacteria biomass in bottom waters was higher than that in surface water. 相似文献
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胶州湾夏季异养浮游细菌的时空变化规律及影响因素 总被引：3，自引：0，他引：3
2001年夏季对胶州湾异养浮游细菌在一个潮周期内的变化规律及影响因素进行了研究。结果表明,胶州湾异养浮游细菌数量的垂直分布特征是表层大于底层,表层平均8.99×109个/L,底层平均5.23×109个/L。胶州湾水体异养浮游细菌日变化幅度在表层水体较为明显,底层相对较小,但其变化规律均为最高值在小潮期而最低值出现在大潮期。浮游动物摄食、浮游植物光合作用产生的溶解有机物及水温和日光中的紫外辐射是影响胶州湾异养浮游细菌昼夜变化的主要因素,水交换是影响其日变化的主要因素。 相似文献
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长江冲淡水区细菌生产力研究 总被引：11，自引：3，他引：11
为了解细菌在长江口冲淡水区生态系营养动力学过程中的重要作用,笔者于1997年10月10日至20日,1998年5月14日至6月1日在观测海区以及在绿华山海域设置大水体围隔生态系实验装置进行细菌生产力的现场观测研究.结果表明,秋季观测海区平均细菌生产力(C)为(1.44±1.30)μg/(dm3·h),高值出现在测区中部的A3,B3和C3站.春季测区表层细菌生产力(2.43±1.22)μg/(dm3·h)高于底层(1.01±0.43)μg/(dm3·h),高值出现在测区中部的A3和B断面诸站.秋、春季平均细菌生产力相当于浮游植物初级生产力的23%.秋季和春季表层细菌数量分别为(5.22×108±0.88×108)个/dm3和(1.97×108±1.10×108)个/dm3.1998年5月18日至6月1日在围隔实验点的自然海区中细菌生产力变幅范围为0.13～5.79μg/(dm3·h),平均值为(2.47±1.60)μg/(dm3·h).围隔装置内加可溶性磷(PO43-)实验,春季细菌生产力由1.28μg/(dm3·h)增长至32.20μg/(dm3·h),其增长幅度低于秋季1.43～43.47μg/(dm3·h).油污染实验中细菌生产力由6.61μg/(dm3·h)增长至37.97μg/(dm3·h),呈逐日上升趋势. 相似文献