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1.
Dissolved fluorescent organic matter was measured using fluorescence excitation-emission matrix spectroscopy at three temporal points during a Synechococcus bloom after diatom bloom dispersion in spring, 2007. Carbohydrate and dissolved organic carbon were also analyzed. The relationship between organic matter and red tide succession was examined. The results show that after the diatom bloom, tyrosine-like fluorescence B intensity was very high within the water column and exhibited a negative correlation wi...  相似文献   
2.
营养盐水平对四种海洋浮游硅藻胞外多糖产量的影响   总被引:10,自引:0,他引:10  
4种海洋浮游硅藻(牟勒氏角毛藻、海链藻、三角褐指藻和新月菱形藻)培养在改进的f/2培养基中,研究了不同氮、磷和硅营养水平对它们胞外多糖产量的影响.结果表明,硅藻胞外多糖的生产和释放具有种间特异性,角毛藻和海链藻胞外多糖的生产和释放主要在静止期,而三角褐指藻和新月菱形藻在指数生长期前期和静止期都能生产和分泌较高的胞外多糖;培养液中低浓度磷减少了4种硅藻在静止期胞外多糖的产量,但增加了角毛藻在生长期前期胞外多糖的产量;氮浓度的降低增加了三角褐指藻在指数生长前期胞外多糖的产量,但减少了其他3种藻类胞外多糖的产量;硅浓度的降低对4种硅藻胞外多糖的产量影响不大,在一定程度上还促进了静止期胞外多糖的生产.本研究表明,营养盐水平对硅藻胞外多糖生产的影响因种类和细胞所处生长期不同而存在着很大的差异.  相似文献   
3.
Abstract. Monthly soluble and insoluble protein, carbohydrate, lipid and ash levels and energetic content were measured in tissues of Paracentrotus lividus adults living in a lagoonal environment over a 1-year period to increase our understanding of resource allocation to the different tissues. The biochemical composition of P. lividus individuals in this environment is similar to that observed in other environments. The gonad and gut contained a high level of proteins soluble or insoluble, a lesser amount of lipids and, finally, a low level of carbohydrates. The main component of the test was ash, followed by protein levels. The lipid and carbohydrate levels in the test were very low. Seasonal variability in the biochemical composition of the different body parts was observed. In the gonad, an increase in the level of lipids was recorded concomitant to an increase in gonad weight. The lipids and carbohydrates, after an initial increase, decreased with the maturation of the gonad. The biochemical variations observed in the gut seem to be linked to both the reproductive cycle and the sea urchin's trophic activity. Variability in the test was low and irregular for all of the observed biochemical components. As P. lividus is an important member of the benthic community in the Urbinu lagoon, this echinoid represents an important store of materials and energy within this lagoonal environment.  相似文献   
4.
In this work, investigations dealing with the determination of hydrocarbons in contaminated soil water are presented. The hydrocarbons under investigation range from low to high volatility compounds. A GC‐FID method was developed that due to its efficiency, routine suitability, relative rapidity, and low cost is suitable for the analysis of complex chemical mixtures of highly volatile hydrocarbons (with boiling points between 69 and 190°C). The standard used was a gasoline mixture with boiling points ranging from 100 to 190°C. For this standard, no supplementary preparation is needed and it is suitable for the whole range of hydrocarbons under investigation. The determination of the hydrocarbon content of the samples was performed applying univariate and multivariate statistical analysis to the experimental data. In the characterization of a contamination with highly volatile hydrocarbons of soil water originating from different depth layers from the chemistry location Leuna (Sachsen‐Anhalt, Germany), the advantages of a multivariate method are demonstrated in exemplary manner.  相似文献   
5.
东方百合低温打破休眠期间碳水化合物及呼吸代谢变化   总被引:1,自引:0,他引:1  
 以东方百合“西伯利亚”为试材,对不同温度(25 ℃和4 ℃)处理下百合外部及内部鳞片的碳水化合物含量和呼吸代谢途径的关键酶进行了测定。结果表明,随着储藏时间的延长,百合淀粉含量不断下降,可溶性糖含量在低温处理中期较高,且外部鳞片变化较内部鳞片变化剧烈,4 ℃低温处理糖化比25 ℃明显;在百合休眠期间,3条主要呼吸代谢途径的关键酶——糖酵解(EMP)途径的丙酮酸激酶(ATP)、三羧酸循环途径(TCA)的琥珀酸脱氢酶(SDH)和磷酸戊糖途径(PPP)的葡萄糖六磷酸脱氢酶(G6PDH)呈现不同的变化趋势。根据不同途径关键酶的变化趋势判断,处理前30 d,3条主要呼吸途径变化较剧烈,EMP占绝对优势且不断升高,TCA和PPP呈不断下降过程,且内部鳞片变化比外部鳞片明显;其后随着休眠处理时间的延长,EMP不断下降,而TCA和PPP不断得到活化;呼吸强度变化趋势与碳水化合物自然休眠解除的进程基本一致。  相似文献   
6.
非结构性碳水化合物(NSC)在植物生长、繁殖、防御和生存等方面发挥着基础性作用。为探究黑果枸杞(Lycium ruthenicum)NSC积累的器官间规律及其对土壤氮磷供应水平的响应,以3年生黑果枸杞为对象,测定了3个氮磷比例和供应量条件下根茎叶NSC含量。结果表明:(1)黑果枸杞根、茎、叶NSC组成均以可溶性糖为主,淀粉约占37.3%;器官NSC含量粗根和细根>叶片>新梢>一二年生枝条,各器官可溶性糖含量粗根和细根>叶片>新梢>一年生枝>二年生枝;淀粉含量细根>粗根>二年生枝>一年生枝>叶片和新梢;糖淀比叶片>新梢>粗根>一年生枝>二年生枝>细根。(2)氮磷比和供应量对黑果枸杞根系可溶性糖含量无显著影响,对茎叶NSC含量的影响存在显著交互效应。氮磷比5∶1时,茎叶NSC含量随氮磷供应量升高而增加;氮磷比15∶1时,茎叶NSC含量随氮磷供应量增加显著降低;氮磷比45∶1时,茎叶NSC含量随供应量的增加而显著升高。(3)一年生枝NSC含量于萌芽前达到最大值(5.05%DW),于春梢旺盛生长期降至最低值(3.79%DW);二年生枝NSC含量在萌芽前最低(4.23%DW),于秋季落叶期达到最大值(4.33%DW)。(4)粗根和叶片NSC含量与一二年生枝NSC含量负相关;新梢NSC含量与叶片NSC、二年生枝淀粉含量正相关;一年生枝可溶性糖含量与二年生枝可溶性糖含量负相关;二年生枝淀粉含量与根系淀粉和NSC含量负相关。黑果枸杞通过调整各器官NSC积累及分配应对生长季各器官能量需求和环境氮磷供应变化,在氮磷限制环境中将更多NSC储存在根系,可平衡当前碳同化与未来能量需求的矛盾,增强个体生存能力,也为黑果枸杞应对非生物逆境胁迫提供更大的灵活性。  相似文献   
7.
以初始体质量(8.77±0.16)g的大菱鲆(Scophthalmus maximus)幼鱼为研究对象,在室内流水系统中进行9周的养殖实验,比较分析饲料中3种不同类型的糖源(葡萄糖、蔗糖和糊精)对大菱鲆脂肪代谢相关酶活性和肌肉脂肪酸组成的影响。结果表明,不同类型的糖源显著影响肝脏中与脂肪合成相关的葡萄糖-6-磷酸脱氢酶(G6PD)和苹果酸酶(ME)的活性(P0.05)。饲料糖源为葡萄糖时,大菱鲆肝脏中G6PD和ME的活性显著高于其他处理组,而蔗糖和糊精处理组之间没有显著差异。对与脂肪分解代谢相关酶的活性分析表明,肠道脂肪酶的活性受饲料中不同糖源的影响显著(P0.05);肝脏脂蛋白脂酶的活性在糖源为葡萄糖时显著低于其他处理组(P0.05);不同糖源对肝脂酶的活性无显著影响(P0.05);糊精组的总脂酶活性显著高于葡萄糖组(P0.05)。葡萄糖组大菱鲆肌肉中的C16∶0和C18∶0的含量显著高于糊精组,而C16∶1和C18∶1n-9的含量显著低于糊精组(P0.05)。研究表明,饲料中不同类型的糖源对大菱鲆肌肉脂肪酸的影响集中在饱和脂肪酸和单不饱和脂肪酸上,而对多不饱和脂肪酸的影响有限。  相似文献   
8.
采用Comell方法研究了初始重为(2.0±0.1)g的重口裂腹鱼幼鱼日粮中常量营养物质的适宜需求量。日粮中蛋白质、脂肪和碳水化合物梯度变化范围分别为33%-51%、3%-18%和24%-33%,试验周期为60d。试验结果表明,除日粮蛋白质33%组鱼成活率明显下降外(P〈0.05),其它试验日粮中3种常量营养物质含量对重口裂腹鱼幼鱼成活率没有显著影响(P〉0.05)。当日粮蛋白质水平≤45%时,其特定生长率随着日粮蛋白质水平上升而明显增加;当日粮蛋白质水平在33%-36%时,重口裂腹鱼的特定生长率显著降低(P〈0.05)。除12%日粮脂肪组试验鱼外,9%日粮脂肪组试验鱼的特定生长率显著性高于其它脂肪水平日粮组(P〈0.05),但当日粮中脂肪含量≥15%后,重口裂腹鱼鱼体脂肪含量显著上升(P〈0.05),肝细胞脂肪变性明显。日粮中不同的碳水化合物水平对重口裂腹鱼特定生长率没有显著影响(P〉0.05),但当日粮中碳水化合物为33%时,鱼体糖原含量显著增加(P〈0.05)。试验表明,重口裂腹鱼幼鱼日粮蛋白质、脂肪和碳水化合物3种常量营养物质的适宜需求量分别为45%、9%和≤30%。日粮总能为19.0MJ/kg,蛋白质能量比(P/E)为23mg/J。  相似文献   
9.
1 INTRODUCTION White shrimp Litopenaeus vannamei, a tropical species, naturally distributes along the eastern Pa- cific coast from northern Mexico to northern Peru along 20℃ isotherms, was introduced into China in 1988 (Cai and Chen, 1995). Temperature w…  相似文献   
10.
The analysis of the protein and carbohydrate in P-POM (Plankton and Particulate Organic Matter) samples collected from the fishing ground in Minnan-Taiwan Bank in five voyages (April, June, July, August and November, 1988) shows that the protein and carbohydrate contents and amounts in samples from four stations (501, 401, 301, 201) along the coast and another four stations (404, 304, 403, 204) south and southeast of the shoal were higher than those in April and November, indicating that this phenomenon is related to the upwelling in the two regions in summer .  相似文献   
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