Evaluation of Sludge Digester Effluent as Feeding Solution for the Enrichment of Anammox Bacteria     

&#x;pek elen‐Erdem 《洁净——土壤、空气、水》2020,48(3)

The aim of this study is to evaluate the feasibility of sludge digester effluent as feeding solution to enrich anaerobic ammonium oxidation (anammox) bacteria. The performance of the two parallel pilot scale‐upflow packed bed anammox reactors (UPBAn1 and UPBAn2) are examined in terms of the enrichment of anammox bacteria. The control experiment is set up conducting synthetic wastewater as feeding solution in the UPBAn1 reactor whereas, the sludge digester effluent is fed to the nitritation reactor and then the partially nitrated digester effluent to the UPBAn2 reactor. Anammox activities are evaluated by mass balances based on ammonium (NH₄⁺), nitrite (NO₂^?), and nitrate (NO₃^?) analysis and NRR. Microbial community of anammox bacteria is analyzed using real‐time polymerase chain reaction (PCR). The results demonstrate that UPBAn 1 and UPBAn2 reactors are successfully enriched on days 64 and 40 with NRRs of 19.54 and 19.43 g N m^?3 per day, respectively. This study reveals that both synthetic wastewater and digester effluent are suitable for the enrichment of anammox bacteria; however, digester effluent as feeding solution for enrichment of anammox bacteria based on the ease of process control and process stability is more advisable.  相似文献   

广西忻城福六浪洼地堵洞成库施工实践          下载免费PDF全文

邓忠  韦兴标  何德顺 《探矿工程》2020,47(11):61-69

为解决广西忻城县城关镇弄长村一带万余人饮水问题及该岩溶石山区干旱农田引水灌溉问题，在广西忻城县隆光地下河福六浪洼地3号天窗下游钻取了投料孔和帷幕灌浆孔，先在投料孔投放碎石骨料，后在帷幕灌浆孔内灌入水泥砂浆或水泥浆液对先期投放的碎石骨料进行固结，修筑地下堵体防渗帷幕对流经福六浪洼地的隆光地下河管道实施堵截，成功地截住了隆光地下河水向下游排泄量，减少了洼地积水通过天窗、落水洞向下游的渗漏量，增加了天窗向洼地的溢洪量，建成了库容约250万m3的溶洼水库，基本解决了城关镇弄长村一带万余人生活饮水难的问题，提高了该溶洼水库周边、岩溶大石山区干旱农田灌溉水平和改善了当地村民的饮水条件。  相似文献   

微塑料对海洋桡足类摄食、排泄及生殖的影响     

于娟  许瑞  魏逾杰  田继远  王雪丹  来敬广  陈容  杨桂朋  张正雨  邵云粹 《中国海洋大学学报(自然科学版)》2020,50(3):73-80

微塑料污染目前成为海洋污染普遍关注的一个研究热点。本文在实验室内将青岛近海常见的海洋桡足类猛水蚤暴露于不同浓度的微塑料尼龙6中,研究了猛水蚤的摄食、排泄以及生殖的变化。研究结果表明,微塑料尼龙6对猛水蚤的摄食、排泄、生殖均产生不利的影响,并且存在剂量-效应关系。微塑料尼龙6对猛水蚤摄食率、滤水率、排粪率的24 h·EC 50分别为67.7、62.2、84.1 mg·L^-1,对猛水蚤抱卵率的144 h·EC 50为30.3 mg·L^-1。“饱食感”造成猛水蚤摄食率降低,从而能量和营养摄入不足可能是导致猛水蚤抱卵率降低的原因。猛水蚤对微塑料的摄食,导致猛水蚤排泄的粪便颗粒小型化,由长椭球体变为短小椭球体,可能与其粘度或物理结构的改变有关。暴露于尼龙6的猛水蚤的粪便体积和沉降速率显著低于未暴露微塑料的对照组。本实验结果对于研究微塑料对海洋桡足类以及滤食性浮游动物的生态毒理影响具有一定的帮助。  相似文献   

不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响     

盛鹏程  周冬仁  韩新荣  胡晓波  叶雪平  徐磊  周聃  吴琦芳  郝贵杰 《海洋与湖沼》2020,51(1):148-155

以乌鳢(Ophiocephalus argus)为研究对象,采用不同日粮饲喂,试验W1组饲喂冰鲜鱼,试验W2组饲喂畜禽内脏。12周后,取肠道内容物,利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性,分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明;W1组和W2组物种注释(operational taxonomic unit,OTU)数目分别为2631和4114条,分类地位明确的细菌种类分别隶属5个属和49个属,W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属(Plesiomonas)、狭义梭菌属(Clostridium sensu stricto)、鲸蜡菌属(Cetobacterium);W2组乌鳢肠道细菌的优势类群是狭义梭菌属(Clostridium sensu stricto)、邻单胞菌属(Plesiomonas)、Spartobacteria、甲基副球菌属(Methyloparacoccus)、杆菌属(Alsobacter)、Saccharibacteria、分支杆菌属(Mycobacterium)。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌,表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢;W1组肠道有益菌OTU丰富度大于W2组,且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。  相似文献   

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay     

Qi-Kang Bo  Yun-Zhao Lu  Hui-Jing Mi  Yan-Guang Yu  De-Xian Gu  Hong-Zheng You  Shuang Hao 《Marine Ecology》2020,41(4):e12594

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.  相似文献   

克氏原螯虾(Procambarus clarkii)对新鲜和不同分解腐烂程度的沉水植物摄食选择性研究     

罗艺涓  张泽慧  高健  焦一滢  王和云  廖明军 《湖泊科学》2022,34(4):1262-1270

原产美国南部和墨西哥东北部的克氏原螯虾是目前全球入侵最广的小龙虾,也是对淡水生态系统最具破坏性的物种之一,其对栖息地沉水植物的现存量构成严重威胁,然而目前对克氏原螯虾摄食沉水植物的机制还知之甚少.本文选择6种沉水植物,研究克氏原螯虾对浅水湖泊常见的沉水植物穗花狐尾藻(Myriophyllum spicatum)、密刺苦草(Vallisneria denseserrulata)、轮叶黑藻(Hydrilla verticillata)、菹草(Potamogeton crispus L.)、马来眼子菜(Potamogeton malaianus)和金鱼藻(Ceratophyllum demersum)的新鲜植株和分解腐烂后的植株的摄食偏好及原因,结果表明克氏原螯虾对沉水植物新鲜植株选择逗留偏好为:最偏爱逗留在轮叶黑藻区,在穗花狐尾藻、密刺苦草和马来眼子菜3种植株区逗留频次无区别,而在金鱼藻区逗留频次显著最低,6种植物湿重减少量与逗留偏好趋势相似;相对各新鲜植株,克氏原螯虾均更喜欢逗留在分解腐烂8 d或12 d后的植物碎屑区域,相应分解腐烂8 d或12 d植物湿重降低量也显著更高.6种植物新鲜植株总酚类含量均显著高于分解腐烂后的植株,其中穗花狐尾藻新鲜和分解腐烂后的植株的总酚类含量始终显著高于其他5种植物.结合植物总酚类指标和摄食偏好结果,表明克氏原螯虾对沉水植物的摄食具有选择性,相对新鲜植物更喜摄食腐烂后的碎屑,总酚类不是影响其选择的关键因子.  相似文献   

海洋环境因子对澳洲鲐亲体补充量关系的影响——基于贝叶斯模型平均法的研究   总被引：1，自引：1，他引：0  

张畅  陈新军 《海洋学报》2019,41(2):99-106

澳洲鲐（Scomber australasicus）是西北太平洋重要的中上层经济鱼类，生命周期相对较短，资源量受补充量影响明显，了解澳洲鲐太平洋群系补充量状况对掌握其资源量及确保其可持续利用具有重要的意义。本文利用产卵场1（30°~32°N，130°~132°E）海表面温度（sea surface temperature，SST1）、产卵场2（34°~35°N，138°~141°E）海表面温度（SST2）、索饵场（35°~45°N，140°~160°E）海表面温度（SST3）、潮位差（tidal range，TR）、太平洋年代际涛动（Pacific decadal oscillation，PDO）和亲体量（spawning stock biomass，SSB）6个影响因子任意组合与补充量构建多个模型，运用贝叶斯模型平均法（Bayesian model averaging，BMA）分析各个环境因子对资源补充量的解释能力，并预测其补充量的变化。结果表明，SSB对补充量具有最长期且稳定的解释能力，其次是SST3，PDO、TR、SST2、SST1也对补充量模型具有一定的解释能力。SST3是环境因子中影响最大的因子，可能是由于补充群体在索饵场内生活时间较长，索饵场温度对仔鱼或鱼卵的生长存活有较大的影响。研究认为，基于BMA的组合预报综合考虑了各个模型的优势，优于单一模型，可用于澳洲鲐资源补充量的预测。  相似文献   

溯河洄游长江刀鲚(Coilia nasus)摄食虾类的调查   总被引：4，自引：0，他引：4  

姜涛  刘洪波  李孟孟  杨健 《湖泊科学》2018,30(2):458-463

刀鲚(Coilia nasus)是长江的名贵经济鱼类.虽然传统上认为其溯河生殖洄游全过程不会摄食,但该问题一直尚未完全弄清.作为有效解明这一问题的第一步,本研究在前期长江流域干流和湖泊刀鲚资源调查的基础上,先利用耳石微化学技术筛选出长江河口区、江苏江段、安徽江段和鄱阳湖水域溯河洄游型的刀鲚个体,再对其胃、肠容物的大型游泳动物(鱼、虾类)进行调查分析.结果发现,在河口区、江苏和安徽江段所有刀鲚个体胃充塞度为0级,均未发现摄食有游泳动物;而鄱阳湖水域刀鲚个体的胃充塞度达4~5级,均发现有摄食淡水虾类的日本沼虾(Macrobrachium nipponense)和秀丽白虾(Exopalaemon modestus)的情况.其中29%和71%的刀鲚胃中分别含有1只和2只虾.所有刀鲚个体肠内均未发现有内容物.鉴于鄱阳湖已被确定为溯河洄游型刀鲚的产卵场之一,结果表明长江刀鲚在经河口,通过长江江苏和安徽江段干流到达鄱阳湖产卵场的过程中应该不会摄食大型游泳动物;而进入鄱阳湖产卵场后会开始摄食日本沼虾和秀丽白虾.这种两阶段的现象可能反映出了刀鲚的一种在洄游通道上节约能量,以利长距离溯河;而在产卵场补充能量,以利于性腺最终成熟的生存策略.  相似文献   

黑海参(Holothuria atra)对环境沉积物的摄食选择及消化系统功能适应性研究     

薛英楼  高菲  许强  黄端杰  王爱民  孙涛 《海洋与湖沼》2019,50(5):1070-1079

黑海参(Holothuria atra)是热带珊瑚礁大型底栖动物群落中的代表种类之一,其摄食活动对珊瑚礁生境底质有机物的循环再利用具有重要作用。本文研究了三亚蜈支洲岛典型热带珊瑚礁海域野生黑海参的沉积物选择特征及其触手和消化系统结构功能的适应性。结果表明,黑海参摄取的沉积物颗粒相对较粗,其中粒径0.25mm部分占到了总重量的96.67%。触手上3—6个乳突形成乳突簇[直径(283.00±40.94)μm],适宜摄取大颗粒沉积物。黑海参胃内含物有机物含量显著高于环境沉积物,证实其对有机物含量的选择性;粒径0.25mm部分有机物含量占摄入总有机物量的75%,与环境沉积物中相同粒度的有机物总量占比接近(76%),表明黑海参选择的沉积物粒径范围有效保证了其高效摄入有机质。黑海参前肠的脂肪酶、淀粉酶、纤维素酶、胰蛋白酶活性最高,同时绝大部分的有机质(98.33%)在前肠被吸收。组织学定量分析表明,黑海参前肠黏膜层厚度最大,占总厚度的56.33%,这与其高效吸收有机质的功能相适应;后肠的肠壁总厚度和黏膜下层、肌层厚度均显著高于前肠和中肠,表明其具有相对更好的弹性和收缩能力,适合于大颗粒有机质的高效输送与排出。研究结果证实,黑海参摄食器官和消化道独特的结构与功能保证了其在珊瑚砂粗颗粒生境中高效摄取有机物。  相似文献   

Macroinvertebrate community structure and feeding interactions along a pollution gradient in Gilgel Gibe watershed,Ethiopia: Implications for biomonitoring     

《Limnologica》2017

Feeding interactions among functional feeding groups (FFGs) of macroinvertebrates are robust indicators of aquatic ecosystem interactions. They provide information regarding organic matter processing, habitat condition and trophic dynamics. In tropical rivers with pronounced wet and dry seasons, macroinvertebrate based ecological monitoring tools are explicitly focused on metrics and indices, while ignoring interactions of FFGs. Therefore, the objective of this study was to investigate the functional feeding type metrics, diversity indices and feeding interactions among FFGs of macroinvertebrates along the water pollution gradient in Gilgel Gibe watershed, Ethiopia. Water quality parameters and macroinvertebrate community attributes were assessed for samples collected from upstream sites (15 sites), urban-impacted stretches (12 sites) and wetland-affected river zones (7 sites) of the watershed during the rainy (July) and dry (February) seasons. To understand the effect of pollution on the feeding interactions, stable carbon and nitrogen isotopes were analyzed. Macroinvertebrate-based diversity indices and functional feeding type metric showed deterioration of ecological integrity at the urban-impacted sites and substantial recovery in the wetland-affected downstream sites. Omnivorous feeding behavior of macroinvertebrates was noted for the upstream sites, whereas clear trophic guilds of FFGs were suggested for the wetland-affected river zones by the stable isotope results. The results of pollution gradient analysis and feeding interactions among FFGs revealed that the urban-impacted sites showed weaker interactions when compared to upstream and wetland influenced sites. This affirms the potential importance of feeding interactions among FFGs of macroinvertebrates in water quality monitoring.  相似文献