厦门沿岸海域2种虾虎鱼染色体核型的比较     

何丽斌  周宸  黄瑞芳  林琪 《台湾海峡》2010,29(4):473-477,594,594

采用鱼鳍细胞空气干燥法制片,Giemsa染色,对厦门沿岸海域2种虾虎鱼的染色体核型进行了分析研究.其中,犬牙缰虾虎鱼（Amoya caninus）的染色体数目为2n=50,臂数为NF=84,有8对中部着丝点染色体（M）,7对亚中部着丝点染色体（SM）,2对亚端部着丝点染色体（ST）和8对端部着丝点染色体（T）.髭缟虾虎鱼（Tridentiger barbatus）的染色体数目为2n=44,臂数为NF=68,有5对中部着丝点染色体（M）,6对亚中部着丝点染色体（SM）,1对亚端部着丝点染色体（ST）和10对端部着丝点染色体（T）.未发现有异形染色体.其中犬牙缰虾虎鱼的染色体数目是文献中尚未报道的1类虾虎鱼染色体类型.  相似文献   

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses     

YOU Feng  WANG Wei  XU Dongdong  ZHU Xiangping  NI Jing  WU Zhihao  XU Yongli  WANG Xincheng  ZHANG Peijun 《中国海洋湖沼学报》2009,27(2)

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.  相似文献   

军曹鱼染色体组型分析          下载免费PDF全文

刘楚吾  曹伏君 《海洋科学》2008,32(1):29-31

为了解军曹鱼(Rachycentron canadum)的细胞遗传学特征,采用植物血球凝集素(PHA)、秋水仙碱胸腔注射,取头肾细胞经空气干燥法制片,分析了军曹鱼染色体核型。结果表明,军曹鱼核型为:2n=48=48t,染色体总臂数(NF)为48。  相似文献   

宽壳全海笋的染色体核型分析     

陆荣茂  柴雪良  董迎辉  肖国强  林志华 《台湾海峡》2008,27(1):43-46

以宽壳全海笋担轮幼虫为材料,采用热滴片法进行染色体核型分析.结果表明:宽壳全海笋二倍体染色体数目为2n=38,染色体臂数(NF)=70,核型公式为24m 8sm 6st,未发现性染色体和随体.与海螂目另一物种砂海螂相比,二倍体染色体数目多4条,亚端部着丝粒染色体(st)多6条,核型比砂海螂更具不对称,推测宽壳全海笋较砂海螂原始.  相似文献   

Karyotype analysis of four nemertean species     

陈海霞  刘可可  孙世春 《中国海洋湖沼学报》2009,27(4):748-752

We performed a karyotype analysis on four species from Phylum Nemertea using regenerating somatic cells. Two palaeonemertean species, Cephalothrix hongkongiensis and Cephalothrix sp., had the same chromosome number (2n=28), but different karyotypes (16 m+10 sm+2 st, NF=54 and 22 m+4 sm+2 st, NF=54, respectively). The karyotypes of the two heteronemerteans, Notospermus geniculatus and Ramphogordius sanguineus were 2n=38, 30 m+6 sm+2 sm/st (NF=76/74) and 2n=56, 48 m+4 sm+4 sm/st (NF=112/108), respectively.  相似文献   

Comparative research on karyotype in three species of Arcidae     

Zheng Jiasheng  Wang Meilin  Guo Danhong  Xu Ximin  Gao Qinglan 《海洋学报(英文版)》1996,15(4):529-538

ＣｏｍｐａｒａｔｉｖｅｒｅｓｅａｒｃｈｏｎｋａｒｙｏｔｙｐｅｉｎｔｈｒｅｅｓｐｅｃｉｅｓｏｆＡｒｃｉｄａｅＺｈｅｎｇＪｉａｓｈｅｎｇ；ＷａｎｇＭｅｉｌｉｎ；ＧｕｏＤａｎｈｏｎｇ；ＸｕＸｉｍｉｎｄａｎｄＧａｏＱｉｎｇｌａｎＡｂｓｔｒａｃｔ：Ｂｙａｉｒ－...  相似文献   

4种石斑鱼染色体核型研究   总被引：15，自引：0，他引：15       下载免费PDF全文

郑莲  刘楚吾  李长玲 《海洋科学》2005,29(4):51-55

以蜂巢石斑鱼(Epinephelus merra)、鲑点石斑鱼(Epinephelus fario)、点带石斑鱼(Epinephelus malabaricus)及黑边石斑鱼(Epinephelus fasciatus)为材料，胸腔注射PHA及秋水仙素溶液，取头肾细胞经空气干燥法制片。经核型分析，蜂巢石斑鱼与鲑点石斑鱼核型一样，2n=48=4m 6sm 4st 34t，NF=62；点带石斑鱼与黑边石斑鱼的核型一样，2n=48=48t，NF=48。由染色体数目2n=48可知，4种石斑鱼在鱼类系统中划分为高位类；由核型组成不同，可以将4种石斑鱼分为两大类：蜂巢石斑鱼与鲑点石斑鱼属于一大类；点带石斑鱼与黑边石斑鱼属于另一大类。此外，按总臂数多少来分，蜂巢石斑鱼与鲑点石斑鱼为特化类群：点带石斑鱼与黑边石斑鱼为原始类群。  相似文献   

三种海产帘蛤目贝类的核型研究   总被引：16，自引：0，他引：16  

孙振兴  郭胜超  邵雁群  秦燕  杨爱国 《海洋学报》2004,26(1):88-94

以大竹蛏、紫石房蛤和中国蛤蜊的鳃组织为材料,经注射植物血球凝集素和秋水仙素处理,低渗作用后,采用滴片法和压片法制片,对3种贝的核型进行了研究.结果表明,3种贝类的二倍体染色体数均为38条,大竹蛏的核型为26m+6sm+2st+4t,NF=0;紫石房蛤的核型为32m+2sm+4st/t,NF=2;中国蛤蜊的核型为20m+16sm+2st/t,NF=4.在3种贝类的染色体中均未发现随体及性染色体.  相似文献   

栉孔扇贝Chlamys farreri(Jones & Preston,1904)染色体核型   总被引：3，自引：0，他引：3  

王梅林  郑家声  余海 《中国海洋大学学报(自然科学版)》1990,(1)

以鳃为材料,用秋水仙素—低渗—空气干燥制片方法,研究栉孔扇贝染色体核型,得出2n=38,NF=76,其中中部着丝点染色体3对,亚中部着终点染色体5对,亚端部着丝点染色体11对。与日本沿岸栉孔扇贝染色体数目相同,但核型不同。  相似文献   

Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)   总被引：2，自引：1，他引：2  

SHA Zhenxi  REN Guocheng  WANG Xianli  WANG N  CHEN Songlin 《海洋学报(英文版)》2010,29(2):81-87

A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus.  相似文献