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本研究以淡水天然水体主要放流鱼类鲢鱼种为实验材料,采用盐酸四环素(TCH)浸泡和药饵投喂两种方式对鲢进行荧光标记,比较这两种TCH处理方法对鲢微耳石的标记效果;测定150、250和350mg/L的浓度条件下浸泡24h鲢血液转氨酶以及肝脏氧化损伤和抗氧化指标的变化。结果显示, TCH浸泡处理比投喂处理对耳石的标记效果更好,在150—350mg/L浓度范围内,浓度越高标记效果越好;TCH浸泡处理24h会引起鲢血清的谷草转氨酶(AST)、谷丙转氨酶(ALT)活性在处理后1d显著升高,2d后降低到对照水平。肝脏氧化损伤指标丙二醛(MDA)水平,抗氧化酶超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均呈现先快速极显著升高,然后下降直至恢复正常水平。表明TCH浸泡对鲢肝脏造成了短暂的功能异常和氧化应激胁迫,但在2—5d后即可恢复正常,TCH浸泡方法是鲢标记放流有效、安全、可靠的方法。本研究结果可为鲢的增殖放流、跟踪监测和效果评估工作提供技术支撑,为其他鱼类的标记放流提供参考。  相似文献   
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Otoliths are biogenic carbonate minerals in the inner ear of teleost fish, whose compositions can record the physical and chemical conditions of the ambient water environment inhabited by individual fish. In this research, the fishbones and otoliths of naked carp sampled near the Bird Island, offshore Lake Qinghai, were dated and analyzed for mineralogy and microchemical compositions. Comparing the microchemical compositions of ancient otoliths with those of modern otoliths, we conclude that the ancient naked carps inhabited a relict lake formed when the lake shrank from a high lake level, by combining with the AMS-14 C ages of fishbones and otoliths, the stratigraphy and surrounding topography of the sample site. AMS-14 C dating results of ancient fishbones and otoliths show that these naked carps lived from 680 to 300 years ago, i.e. during the Ming Dynasty of China. The X-ray diffraction(XRD) patterns demonstrate that the ancient lapillus is composed of pure aragonite, identical to modern one, indicating that the mineral of lapillus didn't change after a long time burial and that the ancient lapillus is suitable for comparative analysis thereafter. Microchemical results show that both ratios of Mg/Ca((70.12±18.50)×10?5) and ? 18O((1.76±1.03)‰) of ancient lapilli are significantly higher than those of modern lapilli(average Mg/Ca=(3.11±0.41)× 10?5 and ? 18O=(?4.82±0.96)‰). This reflects that the relict water body in which the ancient naked carp lived during the Ming Dynasty was characterized by higher Mg/Ca and ? 18 O ratios than modern Lake Qinghai, resulting from strong evaporation after being isolated from the main lake, similar to today's Lake Gahai. Based upon the stratigraphy and altitude of naked carp remains, it can be inferred that the altitude of lake level of Lake Qinghai reached at least 3202 m with a lake area of 4480 km2 during the Ming Dynasty, approximately ~5% larger than it is today.  相似文献   
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通过对北京密云水库的野生鲤鱼的微耳石和星耳石中的蛋白质进行提取,分别得到了3种基质蛋白,根据其在提取过程中溶解性的不同,分为水可溶蛋白(WSP)、酸可溶蛋白(ASP)和酸不可溶蛋白(AIP).通过高效液相色谱、X射线衍射、红外光谱分析和氨基酸成分分析等方法分别对3种基质蛋白进行了表征,发现微耳石和星耳石中3种基质蛋白特征存在差异,并初步推断蛋白质特征的差别与碳酸钙晶体在微耳石和星耳石中的不同晶型有关.  相似文献   
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