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1.
米氏凯伦藻(Karenia mikimotoi)近年在我国福建、浙江和广东沿海经常形成赤潮,其赤潮不仅影响到海洋生态系统的稳定,也严重威胁到水产养殖以及人类生命健康安全。本论文以米氏凯伦藻为研究对象,建立了米氏凯伦藻细胞表面膜蛋白质荧光标记技术和细胞膜蛋白质提取方法,运用荧光差异凝胶电泳技术(2-DDIGE)对膜蛋白质进行了分析,并研究了米氏凯伦藻的膜蛋白质组及其对环境温度变动的响应。实验共鉴定到44个细胞表面膜蛋白,其中有效注释27个,主要为转运蛋白、HSP70蛋白家族和捕光蛋白等。米氏凯伦藻在20°C条件下的细胞生长和光合作用要明显好于16°C和12°C,但16°C和12°C条件下的差别不大,表明低温限制了米氏凯伦藻的生长。当米氏凯伦藻从12°C快速转移至16°C和20°C时,藻细胞密度和光合作用效率短时间迅速降低,但细胞很快即适应温度变化。细胞膜上的转运蛋白和光合作用蛋白在其适应温度变化中起着重要作用。 相似文献
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3.
近30年长江口海域生态系统健康状况及变化趋势研究 总被引：3，自引：0，他引：3
为评估长江口海域生态系统健康状况，本文以鱼类浮游生物为指示生物，选择1986年、1999年、2007年和2016年4个年份的数据，选取鱼类浮游生物总种类数、底栖鱼种类数、水层鱼种类数、低耐污鱼种类数、高耐污鱼种类数百分比、杂食性鱼种类数百分比、虫食性鱼种类数百分比、肉食性鱼种类数百分比、鱼类取样个体数、天然杂交种种类数百分比等10个评价指标，采用1、3、5赋值法计算4个不同年份的河口生物完整性指数(Estuarine Biotic Integrity Index，EBI)，并以此评价近30年长江口海域生态系统健康状况变化趋势。结果显示，1986年、1999年、2007年和2016年4个不同年份的EBI值分别是52、40、36、34，对应的EBI等级分别为“好”、“一般”、“一般?差”、“差”。通过分析EBI年际变化的结果，发现近30年来长江口海域生态系统健康状况呈现先下降，而后稳定在较低水平的趋势，说明长江口海域生态系统亟需及时进行保护和修复工作。 相似文献
4.
Tropical rivers display profound temporal and spatial heterogeneity in terms of environmental conditions. This aspect needs to be considered when designing a monitoring program for water quality in rivers. Therefore, the physico-chemical composition and the nutrient loading of the Upper Mara River and its two main tributaries, the Amala and Nyangores were monitored. Initial daily, and later a weekly monitoring schedule for 4 months spanning through the wet and dry seasons was adopted. Benthic macro-invertebrates were also collected during the initial sampling to be used as indicators of water quality. The aim of the current study was to investigate the physico-chemical status and biological integrity of the Upper Mara River basin. This was achieved by examining trends in nutrient concentrations and analyzing the structure, diversity and abundance of benthic macro-invertebrates in relation to varying land use patterns. Sampling sites were selected based on catchment land use and the level of human disturbance, and using historical records of previous water quality studies. River water pH, dissolved oxygen, electrical conductivity (EC), temperature, and turbidity were determined in situ. All investigated parameters except iron and manganese had concentration values within allowable limits according to Kenyan and international standards for drinking water. The Amala tributary is more mineralized and also shows higher levels of pH and EC than water from the Nyangores tributary. The latter, however, has a higher variability in both the total phosphorus (TP) and total nitrogen (TN) concentrations. The variability in TP and TN concentrations increases downstream for both tributaries and is more pronounced for TN than for TP. Macro-invertebrate assemblages responded to the changes in land use and water quality in terms of community composition and diversity. The study recommends detailed continuous monitoring of the water quality at shorter time intervals and to identify key macro-invertebrate taxa that can be used to monitor changes of the water quality in rivers of the Mara basin as a result of anthropogenic changes. 相似文献
5.
Michael E. McClain Amanda L. Subalusky Elizabeth P. Anderson Shimelis Behailu Dessu Assefa M. Melesse Preksedis M. Ndomba 《水文科学杂志》2014,59(3-4):801-819
AbstractEquatorial rivers of East Africa exhibit unusually complex seasonal and inter-annual flow regimes, and aquatic and adjacent terrestrial organisms have adapted to cope with this flow variability. This study examined the annual flow regime over the past 40 years for three gauging stations on the Mara River in Kenya and Tanzania, which is of international importance because it is the only perennial river traversing the Mara-Serengeti ecoregion. Select environmental flow components were quantified and converted to ecologically relevant hydraulic variables. Vegetation, macroinvertebrates, and fish were collected and identified at target study sites during low and high flows. The results were compared with available knowledge of the life histories and flow sensitivities of the riverine communities to infer flow–ecology relationships. Management implications are discussed, including the need to preserve a dynamic environmental flow regime to protect ecosystems in the region. The results for the Mara may serve as a useful model for river basins of the wider equatorial East Africa region.
Editor Z.W. Kundzewicz; Guest editor M. Acreman 相似文献
6.
利用2017年9月至2018年8月北京20个站降水现象仪采集数据与人工平行观测数据,对降水现象仪进行了对比评估。结果表明:降水现象仪的缺测率为0.01%,空报率为0.6%,捕获率为93.5%,漏报率为27.9%,错报率为10.0%。从统计结果可知,降水现象仪的缺测率、空报率较低,捕获率较高,体现出设备性能良好的一面,漏报率和错报率偏高,表明设备漏识别和错误识别的情况较多,漏报主要发生在弱降水过程中,错报主要出现在毛毛雨的识别上。设备测量和人工观测降水开始时间一致性较好,终止时间一致性偏差;降水现象仪现存问题:在大雨强时易出现雨滴叠加误识别,在小雨强时识别率会降低,在无降水错误输出降水现象方面缺少质控。 相似文献
7.
星基增强系统(satellite based augmentation system,SBAS)通过地球同步轨道卫星实时播发导航卫星星历改正数和完好性参数,以提升用户定位精度和完好性。采用最小方差法解算GPS星历改正数,利用卡方统计进行改正数完好性检核,并依据星历改正数方差-协方差信息计算SBAS用户差分距离误差(user differential range error,UDRE)和信息类型28(message type 28, MT28)等完好性参数。利用中国区域27个监测站的实测数据,首先以国际GNSS服务组织的精密轨道和钟差产品为参考解算星历改正数,结果表明,钟差改正精度优于0.1 m,轨道改正精度优于0.4 m;然后解算广播星历改正数,并生成UDRE和MT28参数,广播星历残余误差卡方检验值均小于告警门限,保证了改正数的完好性;最后利用生成的改正数进行SBAS定位解算,得到定位结果的水平精度优于0.7 m,垂直精度优于1.0 m,对比GPS单点定位,所提算法的水平和垂直方向精度分别提升了30%和40%。 相似文献
8.
根据福清市核电厂候选厂址区花岗岩体微风化和中风化层结构的特征,节理裂隙统计,结合声波测试和室内岩石单轴抗压强度、变形试验、三轴压缩试验等测试数据,评价核电厂地基岩体的适宜性。 相似文献
9.
Xianzhong Cheng Kun Shao Shangyue Shen Shenghong Hu Haiou Qiu 《Geostandards and Geoanalytical Research》2011,35(4):461-469
A rapid and inexpensive method was developed for the determination of trace silver in polymetallic ore samples by use of eggshell membrane (ESM), a natural biomaterial, as the solid‐phase extraction (SPE) adsorbent coupled with flame atomic absorption spectrometry (FAAS). The ESM was used for the separation/pre‐concentration of silver, and the parameters affecting sensitivity, such as pH, sample flow rate, eluent volume and eluent flow rate, were carefully investigated. ESM was found to be an effective solid phase extractant for the adsorption of trace silver over a wide range of acidity from 0.02 to 0.50 mol l?1 HNO3. The sample solution in 0.4 mol l?1 HNO3 was pumped through an ESM microcolumn at the rate of 1.0 ml min?1. Silver was absorbed, and then eluted with a solution of 1.0% m/v thiourea–0.5% v/v HCl. Under these optimal conditions, ESM exhibited a good enrichment efficiency for silver with a dynamic adsorption capacity of 1.7 mg g?1. The proposed method was applied to the FAAS determination of trace silver in polymetallic ores and geological reference materials, GSO‐2, 3 and 5, and GSD‐11, GSD‐12, and the determined values were in good agreement with certified values. 相似文献
10.
R. Mourgues J.B. GressierL. Bodet D. BureauA. Gay 《Marine and Petroleum Geology》2011,28(5):1111-1121
In petroleum industry, the difference between pore pressure (Pp) and minimum horizontal stress Sh (termed the seal or retention capacity) is of major consideration because it is often assumed to represent how close a system is to hydraulic failure and thus the maximum hydrocarbon column height that can be maintained. While Sh and Pp are often considered to be independent parameters, several studies in the last decade have demonstrated that Sh and Pp are in fact coupled. However, the nature of this coupling relationship remains poorly understood. In this paper, we explore the influences of the spatial pore pressure distribution on Sh/Pp coupling and then on failure pressure predictions and trap integrity evaluation. With analytical models, we predict the fluid pressure sustainable within a reservoir before failure of its overpressured shale cover. We verify our analytical predictions with experiments involving analogue materials and fluids. We show that hydraulic fracturing and seal breach occur for fluid pressure greater than it would be expected from conventional retention capacity. This can be explained by the impact of the fluid overpressure field in the overburden and the pressure diffusion around the reservoir on the principal stresses. We calculate that supralithostatic pressure could locally be reached in overpressured covers. We also define the retention capacity of a cover (RC) surrounding a fluid source or reservoir as the difference between the failure pressure and the fluid overpressure prevailing in shale at the same depth. In response to a localized fluid pressure rise, we show that the retention capacity does not only depend on the pore fluid overpressure of the overburden but also on the tensile strength of the cover, its Poisson’s ratio, and the depth and width of the fluid source. 相似文献