发光细菌法在环渤海污水样品遗传毒性检测中的应用     

崔志松  栾晓  高伟  李倩  杨官品  郑立 《海洋与湖沼》2015,46(1):228-232

建立了以经过遗传改造的发光细菌Acinetobacter sp.Rec A为受试物种的环境污染物遗传毒性快速检测方法,该方法最快可在3h内得到毒性评价结果。采用该方法评价了环渤海排污口12份污水样品的遗传毒性。环渤海12个排污口的污水样品均表现出了不同水平的遗传毒性,并呈现出一定的分布特征。其中,高毒水质集中出现在山东半岛污水样品中,中毒水质集中出现在辽东半岛地区,而低毒水质主要集中于京津冀地区。综上,在环渤海的污水样品遗传毒性检测中,这种新型发光细菌法具有快速、灵敏、简便等优点,可为以后近海环境的水质生物毒性检测提供参考依据,具有应用于近海环境水质快速监测与评价的潜力。  相似文献   

Approximate analytical and numerical solutions for radial non‐Darcian flow to a well in a leaky aquifer with wellbore storage and skin effect     

Zhang Wen  Quanrong Wang 《国际地质力学数值与分析法杂志》2013,37(11):1453-1469

This study investigated non‐Darcian flow to a well in a leaky aquifer considering wellbore storage and a finite‐thickness skin. The non‐Darcian flow is described by the Izbash equation. We have used a linearization procedure associated with the Laplace transform to solve such a non‐Darcian flow model. Besides, the Stehfest method has been used to invert the Laplace domain solutions for the drawdowns. We further analyzed the drawdowns inside the well for different cases. The results indicated that a smaller B_D results in a smaller drawdown at late times and the leakage has little effect on the drawdown inside the well at early times, where B_D is a dimensionless parameter reflecting the leakage. We have also found that the flow for the negative skin case approaches the steady‐state earlier than that for the positive skin. In addition, the drawdown inside the well with a positive skin is larger than that without skin effect at late times, and a larger thickness of the skin results in a greater drawdown inside the well at late times for the positive skin case. A reverse result has been found for the negative skin case. Finally, we have developed a finite‐difference solution for such a non‐Darcian flow model and compared the numerical solution with the approximate analytical solution. It has been shown that the linearization procedure works very well for such a non‐Darcian flow model at late times, and it underestimates the drawdowns at early times. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

Biomarkers of Exposure and Effects for Assessing Toxicological Risk of Endocrine Disrupters in Top Predators of the Mediterranean Sea     

M. Cristina Fossi  Silvia Casini  Letizia Marsili  Stefania Ancora  Gabriele Mori  Giovanni Neri  Antonella Ausili  Teresa Romeo  Alessandra Moscatelli  Giuseppe Notarbartolo Di  Sciara 《Marine Ecology》2002,23(S1):184-189

Abstract. Endocrine Disrupting Chemicals (EDCs) have the potential to alter hormone pathways that regulate reproductive processes in wildlife and fisheries. In this research the unexplored hypothesis that Mediterranean top predator species (such as large pelagic fish and marine mammals) are potentially at risk due to EDCs is investigated. In the Mediterranean environment, top predators accumulate high concentrations of polyhalogenated aromatic hydrocarbons (PHAHs) and toxic metals, incurring high toxicological risk. Here we illustrate the need to develop sensitive biomarkers for evaluation of toxicological risk in top marine predators (Xiphias gladius, Thunnus thynnus thynnus) and non-lethal techniques, such as non-destructive biomarkers, for the hazard assessment of threatened species exposed to EDCs, such as marine mammals ( Stenella coeruleoalba, Tursiops truncatus, Delphinus delphis and Balaenoptera physalus ).  相似文献   

水面皮层破坏-复原过程的实验室模拟研究     

吴永森  吴隆业 《海洋与湖沼》2001,32(6):605-611

依据水面红外发射和红外遥感测温原理，采用HDG－高灵敏度红外测温仪和常规测量仪器相结合的方法，在实验室空气稳定条件下，模拟测得了水面皮层破坏－复原（重建）的热力过程和气－水温差对水面皮层复原过程的影响，获得了大量的测量数据。数据分析表明，当气－水温差从3．0℃变为11．5℃时，水面皮层破坏可导致皮温增量从气－水温差3．0℃时的0．15℃变到11．5℃时的0．45℃，并发现恢复时间与气－水温差呈负线性关系。  相似文献   

CHIMEHERB人工皮使用的体会     

任永安  陈长安  刘英娥  陶谏 《中国海洋大学学报(自然科学版)》1989,(Z3)

从1989年4月～1990年10月,我科在69例病人中使用CHIMEHERB人工皮,覆盖治疗了供皮区,Ⅱ°烧伤创面、脱痂创面的残余肉芽创面.临床应用表明:CHIMEHERB人工皮能加快创面的愈合,是目前较好的一种创面覆盖物,用人工皮覆盖创面2～3天后,人工皮干燥,不需再换药,可减轻病人的痛苦.  相似文献   

Estimation of Axial Pile Bearing Capacity According to Shear Strength Parameters of Soil     

Yu Xiaodong  Yan Shuwang Sun Wanhe Postgraduate Student  Tianjin University  Tianjin Professor  Tianjin University  Tianjin Senior Engineer  Tianjin Port Engineering Institute  Tianjin 《中国海洋工程》1997,(2)

At pesent,it is very popular to estimate pile bearing capacity by use of empirical formula andphysical indexes of soil provided in the design codes for civil construction in China.This paper attempts toapply mechanical indexes of soil and semi-empirical formulas,which are based on soil mechanical theoriesand were summarized and presented by Meyerhof in 1976,to calculate the axial pile bearing capacity.Lo-ading test results of 24 single piles in Tianjin area have been collected and compared with the proposed cal-ulation approach.  相似文献   

和田玉子料"肉"与"皮"的关系初探     

张白璐  张小冲  施光海  李新岭 《岩石矿物学杂志》2014,33(S2):25-34

通过对大量和田玉子料的表皮及玉肉的观察与分析以及子料薄片的镜下观察与红外光谱分析,对和田玉子料"肉"与"皮"的关系进行了探讨.子料有以下几种表皮特征:皮色、"汗毛孔"、"砂眼"、裂纹、礓等,玉石内部的特征有礓、杂质、铁花等."皮"与"肉"之间存在一定的对应关系,如:"汗毛孔"的大小会反映玉质的粗细;"砂眼"说明内部有不稳定矿物,如黄铁矿出现处会在表皮形成凹坑;礓出现则往往内外相通,礓的分布特征与玉料品质相关,如星点分布,则严重影响玉石的均匀性.和田玉中的主要组成矿物透闪石也可能表现为礓,其在结构上与其周边玉石迥然不同,不同矿物种类,如透辉石、方解石亦可形成礓.裂纹出现并不都是缺陷,有时会形成沿裂隙分布的鲜艳皮色.以上这些对应关系对于和田玉子料的鉴定、评价以及设计加工都有理论或实践意义.当然目前这些还不能表征和田玉"肉"与"皮"的特征以及对应关系的全部,其中对应关系的原因也有待于深入探讨.  相似文献   

考虑桩-土体系渐进破坏的单桩承载特性研究   总被引：1，自引：0，他引：1  

林春金  张乾青  梁发云 《岩土力学》2014,35(4):1131-1140

针对目前桩基承载特性确定时未考虑桩-土体系渐进变形过程的现状,采用现场试验和理论分析等手段对考虑桩-土体系渐进破坏的单桩承载特性展开研究。首先,利用现场试验数据对侧阻和端阻的软化特性展开深入探讨,提出了侧阻和端阻软化模型,明确了模型中各参数取值方法,并验证了其合理性。同时,根据提出的侧阻和端阻软化模型,建立了一种可考虑桩-土体系渐进破坏的单桩承载特性的迭代算法。算例分析表明,计算获得的破坏性单桩承载特性与实测值有较好的一致性,且可反映侧阻和端阻的破坏特性。实际工程中,结合单桩承载特性分析算法,可根据单桩的实际受力特点灵活选用不同形式的侧阻和端阻荷载传递函数来分析不同桩顶荷载水平下的单桩受力特性。  相似文献   

高桩码头桩基负摩擦力现场试验研究 ——上海洋山深水港工程现场试验   总被引：1，自引：0，他引：1  

肖俊华  周国然  袁聚云  赵锡宏 《岩土力学》2008,29(4):1097-1102

高桩码头接岸结构中的桩基础,在抛石的作用下将产生负摩擦力。通过支撑桩及自由桩上负摩擦力的现场试验研究,获得抛石过程中基桩内力随深度变化的基本规律。抛石过程中基桩上的负摩擦力主要产生在抛石段。同时,抛石作为附加荷载,将引起下方软弱土层的固结沉降,桩土之间产生相对位移,从而在抛石段下方一定范围内也产生负摩擦力作用。承台荷载对负摩擦力的大小和分布有着重要的影响。提出的负摩擦力分布规律和采用有效应力法计算层状土负摩擦力的公式,可为桩基础的设计,尤其对水下单面抛石施工的设计及采取消减负摩擦力的措施提供依据。  相似文献   

工厂化养殖暗纹东方鱼屯致病菌的分离和鉴定     

杨鸢劼  陈辉  方苹  段金荣 《广东海洋大学学报》2005,25(4):18-21

从不同养殖场患病暗纹东方鱼屯(Takifaguobscurus)的鳃、肠、肝、肌肉及溃疡部位分离到9株菌株,经人工感染致病性试验,其T-22、T-11、J-2、H-3、H-5表现出较强的致病性,出现症状与自然发病鱼相同。通过对菌体形态特征、培养特性和API20E细菌生化快速鉴定试条的测定,分别鉴定为温和气单胞菌(Aeromonassobria)、豚鼠气单胞菌(Aeromonascaviae)、嗜水气单胞菌(Aeromonashydrophila)、类志贺邻单胞菌(Plesiomonasshigelloides)、霍乱弧菌(Vibriocholerae)。证实这5株菌为致病菌。药敏试验结果表明9种药物对温和气单胞菌、豚鼠气单胞菌、霍乱弧菌均有较好的抑菌效果;嗜水气单胞菌表现出明显的耐药性。氟嗪酸、环丙沙星、复合磺胺对类志贺邻单胞菌有一定抑菌效果。  相似文献