Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach     

Miller KA  Assunção MG  Dangerfield NJ  Bandiera SM  Ross PS 《Marine environmental research》2005,60(2):153-169

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.  相似文献   

Hepatic CYP1A levels and EROD activity in English sole: biomonitoring of marine contaminants in Vancouver Harbour   总被引：4，自引：0，他引：4  

Miller KA  Addison RF  Bandiera SM 《Marine environmental research》2004,57(1-2):37-54

To assess chemical contaminant stress in the marine environment, ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) expression were measured in 88 English Sole (Pleuronectes vetulus) collected during May and June 1999 from four sites in Vancouver Harbour and at an expected reference site outside the harbour. Hepatic microsomes were prepared from the fish and analyzed for total CYP content, EROD activity, and CYP1A protein levels. Hepatic EROD activity and CYP1A protein levels were elevated in fish from two sites in the inner harbour. A comparison with sediment chemistry data showed that fish with increased EROD activity and CYP1A levels came from sites containing relatively high levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls. Unexpectedly high levels of EROD activity and CYP1A protein were also found in fish from a reference site near Gibsons, in Howe Sound. The elevated EROD activity and CYP1A expression in fish from this site cannot be explained by the chemical analysis data collected.  相似文献   

Multiple wavelength observations of a solar active region     

Franca Chiuderi-Drago  Rino Bandiera  Roberto Falciani  Ester Antonucci  Kenneth R. Lang  Robert F. Willson  Cornelis Slottje 《Solar physics》1982,80(1):71-85

The Solar Maximum Mission Satellite, the Sacramento Peak Vacuum Tower Telescope, the Very Large Array and the Westerbork Synthesis Radio Telescope have been used to observe active region AR 2490 on two consecutive days at soft X-ray, ultraviolet, optical and radio wavelengths (2, 6, and 20 cm), with comparable angular resolution (2 to 15) and field of view (4 × 4). The radio emissions at = 6 cm and 20 cm show a double structure in which one component is associated with bright H plage, C iv and soft X-ray emission, and the other component is associated only with sunspots. No radiation at = 2 cm is detected in this latter component. Coronal temperature and emission measure derived from X-ray lines indicate that the dominant radiation mechanism of the plage-associated component is due to thermal bremsstrahlung while the gyroresonance absorption coefficient must be invoked to account for the high brightness temperature (T _b 2×10⁶K) observed in the sunspot associated component. The high magnetic field strength needed (600 G at a level where T2×10⁶K) is explained assuming a thin transition zone, in order to reach a high electron temperature close to the sunspot, where the magnetic fields are stronger. A higher temperature gradient above sunspots is also consistent with the absence of detectable C iv emission.Cooperative study of the SMY-FBS Project.On leave from the University of Napoli.On leave from the University of Torino.On sabbatical leave 1980–81 at the Arcetri Observatory.On leave from Toyokawa Observatory, Japan.  相似文献   

Slicks detection on the sea surface based upon polarimetric SAR data   总被引：1，自引：0，他引：1  

Bandiera  F. Ricci  G. 《Geoscience and Remote Sensing Letters, IEEE》2005,2(3):342-346

This letter proposes a generalized-likelihood ratio test-based edge detector to be fed by possibly polarimetric synthetic aperture radar (SAR) data. It can be used to detect dark spots on the sea surface and, hence, as the first stage of a system for identification and monitoring of oil spills. The proposed constant false alarm rate (CFAR) detector does not require secondary data (namely pixels from a slick-free area); remarkably, a preliminary performance assessment, carried out by resorting to real SAR recordings, shows that it guarantees detection capabilities comparable to those of previously proposed polarimetric CFAR detectors (which though make use of secondary data). The preliminary performance assessment also seems to indicate that processing polarimetric data does not ensure improved detection capabilities.  相似文献