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A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians 总被引:2,自引:0,他引:2
To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT. 相似文献
2.
Guy Singleton Maria Fay Rola-Rubzen Kado Muir Deeva Muir Murray McGregor 《GeoJournal》2009,74(5):403-413
In spite of a ‘digital divide’, Aboriginal groups in Australia, as internationally, are increasingly using information and
communication technologies (ICTs) to maintain their cultures, communicate, archive knowledge, empower their communities, develop
skills and generate income. Each community uses the technologies differently in accordance with their particular needs and
the opportunities available. The use of ICTs in Aboriginal youth empowerment is illustrated through a case study of an initiative
undertaken by the Walkatjurra Cultural Centre in Leonora, remote Western Australia. A participatory process was used to engage
the Centre’s young people and they were given individual assistance to develop their ICT related capacity. The community conceives
this youth empowerment to be part of a broader youth participation process that will contribute to the Centre’s overall objectives. 相似文献
3.
Michinobu Kuwae Azumi Yamashita Yuichi Hayami Atsushi Kaneda Takashige Sugimoto Yoshio Inouchi Atsuko Amano Hidetaka Takeoka 《Journal of Oceanography》2006,62(5):657-666
In order to examine the responses of primary productivity in the southern coastal sea of Japan to the Pacific Decadal Oscillation
(PDO) in the 20th century, sedimentary records of diatom productivity (diatom valve fluxes) were reconstructed using core
samples from the Bungo Channel (BC) in southwest Japan. The record of the Thalassionema spp. flux—the best index of fall primary productivity in the BC—indicated a multidecadal-scale duration with a low flux (1943–1982)
and those with a high flux (1913–1943 and 1982–2001); apparent shifts were recognized in 1943 and 1982. The shift in 1982
was also recognized in the flux records of other early summer to fall predominant genera in the BC and, previously, in the
biogenic silica records from a broad region of the southeast BC. This indicates that in our records, this shift reflects a
general trend in the primary production in the southeast BC. A comparison among the Thalassionema spp. flux records, meteorological data from an observatory adjacent to the core site, and the PDO index showed that the flux
records were more similar to the PDO index than the other meteorological records, which suggests that the multidecadal-scale
variability of the BC primary productivity may be associated with some marine-derived forcing. The bottom intrusions of nutrient-rich
water that upwelled from the shelf slope into the BC, the axis movement or the transport of the Kuroshio Current off the BC,
and a basin-scale wind stress in the North Pacific might play an important role in this forcing and mediate between the BC
primary productivity and the PDO. 相似文献
4.
Hodaka Kawahata Hideaki Ohshima Azumi Kuroyanagi 《Journal of Asian Earth Sciences》2011,40(6):430-1202
The Mixed Water Region (MWR) between the Oyashio Current and the Kuroshio Extension and surrounding terrestrial region have been affected by the global climatic change. The sedimentary core KR02-15 PC6 collected at 40°23.8871′N, 143°29.8663′E in the MWR provides detailed records of pollen and spores and alkenone sea surface temperature (SST) during the last 15 kyr, showing that both ocean and terrestrial environments have been appreciably linked. Abundance of Picea, a proxy for terrestrial cool climate, fluctuated, coinciding with the alkenone-SST change during the interstadial period (15.0–7.7 cal kyr B.P.). Alkenone-SST increased to the modern level at 8.0 cal kyr B.P. and remained almost constant until 2.0 kyr B.P. while the terrestrial warming still continued until 4.0 cal kyr B.P. The rainfall under terrestrial warm condition increased gradually from 7.9 cal kyr B.P. and rapidly from 2.0 cal kyr B.P. Both terrestrial and marine environments cooled down during the last 2 kyr possibly due to the intensified cold Oyashio Current. 相似文献
5.
Intellectual property rights (IPR) to traditional knowledge (TK) about Australian native plants could justifiably be described
as a birthright of Australian Aboriginal people. However, as is generally the case throughout the world, this right is currently
not protected under Australian law. A case study is presented describing the approach that was taken to protecting the plant
knowledge rights of traditional knowledge (TK) holders who participated in research at Titjikala, central Australia. From
a review of international efforts aimed at developing a legal regime for protection of traditional knowledge (TK) intellectual
property rights (IPR), we found that protecting traditional knowledge (TK) of plants and their uses is most likely to be achieved
through the development of effective protocols for preserving and recording traditional knowledge (TK) and the use of contract
law in commercial applications of that knowledge. The process of negotiating a research agreement between the research organisations
and the community is described. Relationship building and a partnership approach based on trust and mutual respect were found
to be of fundamental importance. The negotiated agreement ensures that the community has an equal share to research partners
in any benefits that might arise from commercialisation of research findings. The approach to benefit sharing developed in
this project has application in other research projects in which the sharing of traditional knowledge (TK) is a fundamental
and essential component of the research process. 相似文献
6.
Akinori Takami Teruo Kondo Azumi Kado Seiichiro Koda 《Journal of Atmospheric Chemistry》2001,39(2):139-153
Uptake coefficient of I2 on aqueous surfaces was measured by using an impinging flow method. Dependence of the uptake coefficient on the gas-liquid contact time and pH was investigated. The uptake coefficient was (3.7± 2.0)×10–4 at 120 ms, 293 K and pH = 5.6. In the alkaline region, the uptake coefficient was larger by one order of magnitude than that in the neutral and acidic region. The I2 uptake on KI solutions and synthetic sea water solutions was also measured. According to the analysis by the reactive uptake model, the uptake of I2 was shown to be mainly determined by liquid phase reactions and the accommodation coefficient of I2 on aqueous surfaces was estimated to be 0.01. 相似文献
7.
Reina Nakaoka Soko Kado Shuichi Hasegawa Keiko Suzuki-Kamata Osamu Ishizuka Shun Sekimoto Hiroshi Kawabata Yoshiyuki Tatsumi 《Island Arc》2021,30(1):e12378
Ar-Ar ages, and petrographical and geochemical characteristics of pyroclastics and an overlying lava from Teshima Island, southwest Japan are presented. Although previous geological and age data suggested Teshima pyroclastics were products of magmatism > 3 my prior to lava flows of Setouchi volcanic rocks generated in association with southward migration of the southwest Japan arc sliver during opening of the Sea of Japan backarc basin at ~ 15 Ma, the present results led to the conclusion that a sequence of Setouchi volcanism, induced by slab melting and subsequent melt-mantle reactions, produced both pyroclastics and lava at 14.6–14.8 Ma. This age is oldest among those reported so far and may represent the timing of onset of characteristic Setouchi magmatism immediately posterior to and hence as a result of the mega-tectonic event including rotation of the southwest Japan arc sliver. 相似文献
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