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1.
Akira Shibata Kouichi Ohwada Masakazu Tsuchiya Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97
Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate
organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted
for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present
study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater
cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size,
0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and
correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG
to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m,
which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell−1 obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29)
and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest
that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas
this fraction was reduced in the middle layer. 相似文献
2.
3.
Ryo Sugimoto Akihide Kasai Toshihiro Miyajima Kouichi Fujita 《Journal of Oceanography》2008,64(1):39-48
Temporal changes in nitrogen isotopic composition (δ15N) of the NO3
− pool in the water column below the pycnocline in Ise Bay, Japan were investigated to evaluate the effect of nitrification
on the change in the δ15N in the water column. The δ15N of NO3
− in the lower layers varied from −8.5‰ in May to +8.4‰ in July in response to the development of seasonal hypoxia and conversion
from NH4
+ to NO3
−. The significantly 15N-depleted NO3
− in May most likely arose from nitrification in the water column. The calculated apparent isotopic discrimination for water
column nitrification (ɛnit = δ15Nsubstrate − δ15Nproduct) was 24.5‰, which lies within the range of previous laboratory-based estimates. Though prominent deficits of NO3
− from hypoxic bottom waters due to denitrification were revealed in July, the isotopic discrimination of denitrification in
the sediments was low (ɛdenit = ∼1‰). δ15NNO3 in the hypoxic lower layer mainly reflects the isotopic effect of water column nitrification, given that water column nitrification
is not directly linked with sedimentary denitrification and the effect of sedimentary denitrification on the change in δ15NNO3 is relatively small. 相似文献
4.
Yoshida A Nomura H Toyoda K Nishino T Seo Y Yamada M Nishimura M Wada M Okamoto K Shibata A Takada H Kogure K Ohwada K 《Marine pollution bulletin》2006,52(1):89-95
Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups. 相似文献
5.
Boundary-Layer Meteorology - Surface shear stresses produced by wind and particle collision play a key role in aerodynamic entrainment and splash processes. The fluid shear stress at the surface... 相似文献
6.
Kunihiro T Miyazaki T Uramoto Y Kinoshita K Inoue A Tamaki S Hama D Tsutsumi H Ohwada K 《Marine pollution bulletin》2008,57(1-5):68-77
We monitored seasonal changes of the abundance and composition of microorganisms in the fish-farm sediment in Kusuura Bay, Amakusa, Japan, using the quinone profiling technique, during bioremediation by introducing cultured colonies of polychaete, Capitella sp. I. In November 2004, approximately 9.2 million cultured worms were transferred to the fish-farm sediment, which increased rapidly, and reached 458.5 gWW/m(2) (528,000 indiv./m(2)) in March 2005. During this fast-increasing period of Capitella, the microbial quinone content of the surface sediment (0-2 cm) also increased markedly, and reached 237 micromol/m(2) in January 2005, although the water temperature decreased to the lowest levels in the year. Particularly, the mole fraction of ubiquinone-10 in total quinones in the sediment, indicating the presence of alpha subclass of Proteobacteria, increased by 9.3%. These facts suggest that the bacterial growth was enhanced markedly by the biological activities of worms in the sediment, and the bacteria played an important role in the decomposition of the organic matter in the sediment. 相似文献
7.
Transport of oceanic nitrate from the continental shelf to the coastal basin in relation to the path of the Kuroshio 总被引：1，自引：0，他引：1
Ryo Sugimoto Akihide Kasai Toshihiro Miyajima Kouichi Fujita 《Continental Shelf Research》2009,29(14):1678-1688
Hydrographic and biogeochemical observations were conducted along the longitudinal section from Ise Bay to the continental margin (southern coast of Japan) to investigate changes according to the Kuroshio path variations during the summer. The strength of the uplift of the cold deep water was influenced by the surface intrusion of the Kuroshio water to the shelf region. When the intrusion of the Kuroshio surface water to the shelf region was weak in 2006, the cold and NO3−-rich shelf water intruded into the bottom layer in the bay from the shelf. This bottom intrusion was intensified by the large river discharge. The nitrogen isotope ratio (δ15N) of NO3− (4–5‰) in the bottom bay water was same as that in the deeper NO3− over the shelf, indicating the supply of new nitrogen to the bay. The warm and NO3−-poor shelf water intruded into the middle layer via the mixing region at the bay mouth when the Kuroshio water distributed in the coastal areas off Ise Bay in 2005. The regenerated NO3− with isotopically light nitrogen (δ15N=−1‰) was supplied from the shelf to the bay. This NO3− is regenerated by the nitrification in the upper layer over the shelf. The contribution rate of regenerated NO3− over the shelf to the total NO3− in the subsurface chlorophyll maximum layer in the bay was estimated at 56% by a two-source mixing model coupled with the Rayleigh equation. 相似文献
8.
Morphological characteristics of snow ripples formed by drifting snow were investigated as functions of wind velocity in a cold wind tunnel at -15 °C. Wave-length, wave height and migration rate of snow ripples increased from 5 to 20 cm, 3 to 5 mm and 1 to 8 cm/min, respectively, with increasing wind velocity from 5 to 7 m/s. Measured size distributions of snow particles in snow ripples showed sorting of large particles in ridges, suggesting that the snow ripple migration is caused by creeping of large particles. The snow drift rate caused by creep, that is, by the ripple migration, was estimated to amount, at least, to 6% of the total snow drift rate. 相似文献
9.
Kouichi Kawaguchi 《Journal of Oceanography》1973,29(3):113-120
Basing upon the total of 1,081 samples collected by a large plankton net (160 cm in mouth diameter) in the Pacific Ocean, the geographical and vertical distribution ofGonostoma gracile were studied. The species is distributed in the water masses of the Kuroshio, the Kuroshio Extension, the North Pacific Current, the Oyashio, the North Pacific Subarctic Water and the western North Pacific Central Water. The center of distribution lies in the Kuroshio area off Japan. Vertically, the species occurs between the depths of 200 and 1,000 m, mainly 300–700 m, both during daytime and at night. A part of population might come up to 0–200 m at night, although its biomass is negligibly small in comparison to that remaining in 300–700 m layer. Postlarvae are found in 300–700 m layer, mainly at 300–500 m, and apparently do not undertake diurnal vertical migration. 相似文献
10.