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Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   
3.
一类广义Sine-Gordon方程的概周期解   总被引:4,自引:0,他引:4  
用最大值原理和Banach压缩映像原理研究一类广义Sine-Gordon方程的概周期解问题,证明概周期解的存在‖u‖L∞<π2中的唯一性。  相似文献   
4.
柯朴 《海洋科学》1978,2(3):57-59
一、什么叫潮汐? 浩瀚的大海和宇宙一切事物一样,永无息止地运动着。它的运动形势是多种多样的。其中有这样一种形式,就是:在一定的时间里,海面会慢慢地升起来,使岸边大片的海滩和礁石被水淹没,而在达到一定高度的时候,海面又开始下落,逐步把淹没的地方裸露出来,而后,又开始上升,并接连不断地重复着这种起落。它是那样的有规律,好像在呼吸一样。人们把这种现象叫做潮汐,同时又把海面上升的过程称为涨潮,下降的过程称为落潮;海面上升的最高点和下降的最低点分别称为高潮和低潮。  相似文献   
5.
Research was conducted to assess the impact of oiling on fresh-marsh plant communities and to test the efficacy of techniques that may be used to enhance the bioremediation of crude oil spills in these environments while minimizing secondary anthropogenic impacts. To emulate field conditions, a mesocosm facility was used that houses 120 mesocosm vessels, each of 200-1 capacity. A five-way factorial treatment arrangement was used that included two substrates (inorganic, organic), two nutrient regimes (fertilized, not fertilized), two aeration levels (substrate aeration, no aeration), three oiling concentrations (0-, 5-, 10-1 m(-2) of South Louisiana Sweet Crude oil), and four vascular plant species (Alternanthera philoxeroides, Panicum hemitomon, Phragmites australis, Sagittaria lancifolia, and an unplanted control). Under the 5- and 10-1 m(-2) oiling concentrations, S. lancifolia displayed a short-term response of increased productivity, whereas P. hemitomon had the highest biomass production and photosynthetic rates at the end of the 18-month experiment. Overall plant growth and productivity, as well as oil degradation, were significantly higher in the inorganic substrate, indicating that biodegradation of oil spills in organic substrates may require a longer time period. Time-released fertilizer also stimulated plant productivity and resulted in higher soil respiratory quotients, suggestive of greater microbial activity, particularly in aerated mesocosms. The amount of oil remaining after 18 months was lowest in aerated and fertilized mesocosms containing either P. hemitomon or S. lancifolia and a substrate of low organic matter content.  相似文献   
6.
考虑一类二阶非线性常微分方程的边值问题(p(t)u′)′+h(t)f(u)=0,0<t<1和u(0)=u(1)=0。通过引入f(s)/s在∞与0处的极限值,并运用打靶法和相应的Sturm比较定理得到解的多重性定理,推广有关文献中的许多重要结果。  相似文献   
7.
Perennially ice-covered lakes are found in the McMurdo Dry Valleys of southern Victoria Land, Antarctica. In contrast to temperate lakes that have diurnal photic periods, antarctic (and arctic) lakes have a yearly photic period. An unusual feature of the antarctic lakes is the occurrence of O2 at supersaturated levels in certain portions of the water column. Here we report the first sediment O2 profiles obtained using a microelectrode from a perennially ice-covered antarctic lake. Sediment cores collected in January and October 1987 from Lake Hoare in Taylor Valley show oxygenation down to 15, and in some cases, 25 cm. The oxygenation of sediments several centimeters below the sediment-water interface is atypical for lake sediments and may be characteristic of perennially ice-covered lakes. There is a significant difference between the observed January and October sediment O2 profiles. Several explanations may account for the difference, including seasonality. A time-dependent model is presented which tests the feasibility of a seasonal cycle resulting from the long photoperiod and benthic primary production in sediments overlain by a highly oxygenated water column.  相似文献   
8.
"This paper establishes and discusses briefly regional patterns and trends in crude birth, death, and natural increase rates in Russia and the USSR from the turn of the century to 1989. The study is based on a comparable regional framework and placed in the context of the demographic transition theory. It discusses sources, collection, and quality of data, as well as necessary estimating procedures. An attempt also is made to examine briefly regional age-standardized rates."  相似文献   
9.
利用海峡两岸及邻近地区暴雨试验期间的时间加密观测资料,分析了1998年5月24日发生在广东省的一次暴雨过程。发现湿有效能量与此次暴雨降水的关系比较清楚。降水开始前,暴雨区的能量显著增加,强降水开始于能量显著减少时;强降水时,暴雨区的能量出现小幅反弹;当暴雨区能量再次显著减少后暴雨降水结束;这是一次能量锋暴雨,暴雨开始前有能量锋生现象发生,暴雨强降水时段与强能量锋同步,暴雨区位于高能舌北侧能量锋附近。  相似文献   
10.
在过去的十年中,显而易见,大气是许多自然物质和污染物从大陆输送到海洋的重要途径。这些物质的大气输入对海洋生物过程和海洋化学循环有正或负的影响。例如:很明显,大气是许多区域的重要营养成分如铁,氮等的重要输送途径。在本报告中,我们评价了这些地区的期的数据,发展向海洋的微量元素,矿物气溶胶,含氮物质合成有机物的大气通量的全球范围的估算值;比较了这些物质通过大气和河流输入的海洋比率。  相似文献   
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