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Subsurface video footage can be used as a successful identification tool for various marine organisms; however, processing of such information has proven challenging. This study tests the use of automated software to assist with photo-identification of the great white shark Carcharodon carcharias in the region of Gansbaai, on the south coast of South Africa. A subsurface photo catalogue was created from underwater video footage. Single individuals were identified by using pigmentation patterns. From this catalogue, two images of the head for each individual were inserted into automated contour-recognition software (Interactive Individual Identification System Beta Contour 3.0). One image was used to search the database, the other served as a reference image. Identification was made by means of a contour, assigned using the software to the irregular border of grey and white on the shark's head. In total, 90 different contours were processed. The output provided ranks, where the first match would be a direct identification of the individual. The method proved to be accurate, in particular for high-quality images where 88.24% and 94.12%, respectively, were identified by two independent analysts as first match, and with all individuals identified within the top 10 matches. The inclusion of metadata improved accuracy and precision, allowing identification of even low-quality images.  相似文献   
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Roach, goby and sturgeon were examined for cytochrome P4501A (CYP1A) expression and histopathology, in relation to contaminant burdens in fish and sediment. Gradients of induction of CYP1A were observed. Roach from the Ural and Ily River Deltas and roach and goby from the two stations nearest the Caspian Sea oil fields displayed higher levels of CYP1A expression in several organs than was observed in fish from further offshore. Great sturgeon and Russian sturgeon showed higher levels of CYP1A expression than was seen in starred sturgeon and gobies in the Ural delta. No fish showed evidence of contaminant-related histopathologies in the organs examined, despite the elevated CYP1A levels. Low levels of polychlorinated biphenyls and elevated levels of inshore and riverine petroleum hydrocarbons from these habitats suggest that this ongoing hydrocarbon exposure, and that from natural sources and long-term oil exploration on the Northeastern Caspian shore, contributed to the CYP1A induction observed.  相似文献   
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