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Acute and severe hypoxia associated with harmful algal bloom has become one of the major causes for the environmental deterioration of coastal areas. Although it is generally thought that a large part of the dissolved oxygen consumption at a bloom site is initiated by heterotrophic bacteria that attack organic matter derived from dead or dying algal cells, precise microbial processes leading to the hypoxia are yet to be examined. Here we show temporal dynamics of extracellular dissolved organic carbon (DOC) of the red tide forming raphidophyte Chattonella marina and bacterial populations associating with the algae under laboratory conditions. During the growth of non-axenic strains of C. marina, we monitored abundance of algae, associated bacteria, and DOC in the culture media. Bacterial cell abundance increased in response to the increase in DOC both at the beginning and the late log phase of the algal growth. Flow cytometric analysis revealed that transient increase in the percentage of respiratory-active bacterial cells also coincided with the timing of the increase in bacterial abundance and DOC. These results strongly suggest that DOC released from growing C. marina fuels respiration and growth of planktonic bacteria surrounding the algae. This has implications for the role of DOC released from C. marina bloom before the collapse in mediating interactions between neighboring algae and bacterial assemblage which may eventually lead to algal bloom-associated hypoxia.  相似文献   
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To compare water quality in rivers of developed and developing countries, a study based on physicochemical parameters and dissolved metals levels was conducted. Water samples were collected from selected sites in Dhaka, Bangladesh; Hokkaido and Osaka, Japan; Erdenet, Mongolia and West Java, Indonesia. Analysis of least significant differences revealed that most water quality parameters were within comparable low levels in both developed and developing countries. The dissolved metals concentrations were found to be similar and below those of water standards except for manganese and cadmium at every sampling point, and lead in Erdenet, Mongolia. Some metals showed high enrichment factors in the rivers of Osaka, Japan and Erdenet, Mongolia, indicating accumulation possibility of metals in the river‐bed sediments. High concentrations of dissolved organic carbon, Escherichia coli and dissolved metals suggested greater water pollution in some rivers of developing countries than in the rivers of Japan. Principal component analysis showed strong correlations between “dissolved organic carbon and chemical oxygen demand” and “conductivity and total dissolved solids” at each sampling point, and E. coli, nitrate (NO), nitrite (NO), and pH levels were found to be higher in the rivers of Dhaka and Erdenet. In addition, there were high levels of Al and Zn in West Java, Pb in Erdenet, and Mn, Fe, and Cr in the rivers of Dhaka and Japan. Based on pressures and impacts, it is evident that dissolved metal, organic, and fecal pollution in the rivers of developing countries are in somewhat dreadful condition in comparison with the rivers of developed country.  相似文献   
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The Mahneshan Metamorphic Complex (MMC) is one of the Precambrian terrains exposed in the northwest of Iran. The MMC underwent two main phases of deformation (D1 and D2) and at least two metamorphic events (M1 and M2). Critical metamorphic mineral assemblages in the metapelitic rocks testify to regional metamorphism under amphibolite‐facies conditions. The dominant metamorphic mineral assemblage in metapelitic rocks (M1) is muscovite, biotite I, Garnet I, staurolite, Andalusite I and sillimanite. Peak metamorphism took place at 600–620°C and ∼7 kbar, corresponding to a depth of ca. 24 km. This was followed by decompression during exhumation of the crustal rocks up to the surface. The decrease of temperature and pressure during exhumation produced retrograde metamorphic assemblages (M2). Secondary phases such as garnet II biotite II, Andalusite II constrain the temperature and pressure of M2 retrograde metamorphism to 520–560°C and 2.5–3.5 kbar, respectively. The geothermal gradient obtained for the peak of metamorphism is 33°C km−1, which indicates that peak metamorphism was of Barrovian type and occurred under medium‐pressure conditions. The MMC followed a ‘clockwise’ P–T path during metamorphism, consistent with thermal relaxation following tectonic thickening. The bulk chemistry of the MMC metapelites shows that their protoliths were deposited at an active continental margin. Together with the presence of palaeo‐suture zones and ophiolitic rocks around the high‐grade metamorphic rocks of the MMC, these features suggest that the Iranian Precambrian basement formed by an island‐arc type cratonization. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   
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One of the key limit states of buckling‐restrained braces (BRBs) is global flexural buckling including the effects of the connections. The authors have previously proposed a unified explicit equation set for controlling the out‐of‐plane stability of BRBs based on bending‐moment transfer capacity at the restrainer ends. The proposed equation set is capable of estimating BRB stability for various connection stiffnesses, including initial out‐of‐plane drift effects. However, it is only valid for symmetrical end conditions, limiting application to the single diagonal configuration. In the chevron configuration, the out‐of‐plane stiffness in the two ends differs because of the rotation of the attached beam. In this study, the equation set is extended to BRBs with asymmetric end conditions, such as the chevron configuration. Cyclic loading tests of the chevron configuration with initial out‐of‐plane drifts are conducted, and the results are compared with the proposed equation set, which is formulated as a function of the normalized stiffness of the attached beam. © 2016 The Authors. Earthquake Engineering & Structural Dynamics published by John Wiley & Sons Ltd. © 2016 The Authors. Earthquake Engineering & Structural Dynamics published by John Wiley & Sons Ltd.  相似文献   
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