Comparative toxicities of chlorinated biphenyls on sea urchin egg fertilisation and embryogenesis     

G. Pagano  M. Cipollaro  G. Corsale  A. Esposito  E. Ragucci  G.G. Giordano  N.M. Trieff   《Marine environmental research》1985,17(2-4)

The aim of this study was to compare the biological actions of selected chlorinated biphenyls (CB's) (with different degrees of chlorine substitution), using the sea urchin test system, for their ability to affect fertilisation, embryogenesis and mitotic activity. Sea urchin embryos were exposed to the test agents throughout larval development or for shorter intervals (e.g. before or after hatching). Sperm or eggs were exposed before fertilisation, thereafter the contaminants were removed from embryo cultures. The results showed maximal activities from medium-chlorinated (tetra- and hexachloro-substituted) compounds, as compared to low- and high-chlorinated biphenyls (mono-, di- and octachloro-substituted), at nominal levels ranging from 10⁻⁶m to 10⁻⁵m. This held true for the different parameters and treatment schedules. Developmental defects were only detected following post hatching exposure of embryos to CB's, whereas pre-hatching exposure failed to reveal any embryological or cytogenetic abnormalities.  相似文献   

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Polycyclic aromatic hydrocarbons in water, seston and copepods in a harbour area in the Western Mediterranean (Ligurian Sea)   总被引：2，自引：0，他引：2  

Luigi Pane  Simona Boccardo  Fabio Bonfiglioli  Gian Luigi Mariottini  Francesca Priano  & Osvaldo Conio 《Marine Ecology》2005,26(2):89-99

Polycyclic aromatic hydrocarbons (PAHs) are widespread carcinogenic, mutagenic and teratogenic pollutants that can significantly affect ecosystems and exert an influence on marine planktonic and benthic communities as well as on higher organisms such as fish and mammals, including humans. In this paper the concentrations of six PAHs in water, seston and copepods were examined in a harbour zone of the Genova Gulf (Ligurian Sea) from November 1997 to June 1998. The importance of freshwater inputs coming from a polluted stream and the influence of seasonal and atmospheric factors were also considered. Highest PAH values were recorded during spring. Fluoranthene, Benzo[b]fluoranthene and Benzo[k]fluoranthene were the most abundant PAHs either in water, seston or copepods. The concentration of total PAHs in seawater was often lower than the detection limit: recorded values ranged between 0.001 and 0.06 ppb. In seston, total PAHs ranged between 0.033 and 6.26 ng·g^?1 and in copepods between 0.01 and 2.34 ng·g^?1. With this study, PAH contamination in the harbour zone of Genova Sestri‐Ponente and the importance of inputs of organic pollutants coming from land and freshwaters have been emphasized.  相似文献   

Plio-Quaternary continental deposits of the Castellamonte area, between Orco and Dora Baltea Basins (Torino Province, Italy)     

Maria Gabriella Forno  Simona Ferrando 《Quaternary International》2008,190(1):103

New geologic mapping of the area near Castellamonte (Torino Province) describes a wide Plio-Quaternary continental succession, up to about 100-m thick. This sequence is distinguished according to allostratigraphic criteria. Altitude, degree of pedogenesis and sediment supply from different basins are also used. Within a single alloformation, several lithofacies are described.Fine-grained fan-delta sediments (Vespia Alloformation) are described in the lower part of the continental succession. The fossil plant remains indicate an Early–Middle Pliocene age. These sediments show an evident deformation and fill ancient lacustrine basins, likely associated with the Canavese Line trend. Their wide distribution and considerable thickness suggest subsidence.Coarse-grained glacial, outwash and fluvial sediments (Colleretto Castelnuovo, S. Defendente, Case Musso, Pagliero, Serra and Piverone Alloformations) are described in the upper part of the continental succession. They consist of undeformed sediments deposited during Middle to Late Pleistocene. These sediments indicate the presence of small local glaciers along the current valley trends. The terracing ratios among the different units and their small thicknesses indicate subsequent differential uplift.  相似文献   

Hydraulic characterisation of some arenaceous rocks of Molise (Southern Italy) through outcropping measurements and Lugeon tests     

M. Nappi  L. Esposito  V. Piscopo  G. Rega 《Engineering Geology》2005,81(1):54-64

  相似文献   

The influence of physical and biological processes on the ichthyoplankton communities in the Gulf of Sirte (Southern Mediterranean Sea)          下载免费PDF全文

Angela Cuttitta  Sergio Bonomo  Salem Zgozi  Angelo Bonanno  Bernardo Patti  Enza Maria Quinci  Marco Torri  Mohamed Hamza  Abdul Fatah  Daw Haddoud  Akram El Turki  Abdul Bari Ramadan  Simona Genovese  Salvatore Mazzola 《Marine Ecology》2016,37(4):831-844

This paper reports on the first study on the ichthyoplankton community structure in the Gulf of Sirte and its spatial distribution in relation to environmental conditions and zooplankton abundance. The results make an important contribution to the future management of fisheries in this unexplored, but much exploited, area. Biological samples were collected in July 2008 using a Bongo40 net. In total, 1914 larvae were found and 1652 of these were identified. In particular, bathypelagic taxa were the most abundant, followed by demersal, mesopelagic, pelagic and epipelagic taxa. The ichthyoplankton community had a patchwork distribution influenced by oligotrophic conditions, the bottom depth and oceanographical features. The results suggest that environmental forcings were able to transport the ichthyoplankton to productive areas. Indeed, maximum fish egg densities were found in coastal stations in correspondence with the Atlantic Tunisian Current inflow, whereas larvae were mainly concentrated in the east side of the Gulf, probably as a result of advection by the anticyclonic circulation. Additionally, the distribution patterns of the total larvae density and the different assemblages were well matched with the abundance of the zooplankton, probably determining final larval survival, growth and recruitment.  相似文献   

Climate and human land-use as a driver of Lake Narlay (Eastern France,Jura Mountains) evolution over the last 1200 years: implication for methane cycle     

Simon Belle  Valérie Verneaux  Laurent Millet  David Etienne  Andrea Lami  Simona Musazzi  Jean-Louis Reyss  Michel Magny 《Journal of Paleolimnology》2016,55(1):83-96

  相似文献   

Impact of drilling mud on chemistry and microbiology of an Upper Triassic groundwater after drilling and testing an exploration well for aquifer thermal energy storage in Berlin (Germany)     

Simona Regenspurg  Mashal Alawi  Guido Blöcher  Maria Börger  Stefan Kranz  Ben Norden  Ali Saadat  Traugott Scheytt  Lioba Virchow  Andrea Vieth-Hillebrand 《Environmental Earth Sciences》2018,77(13):516

After completion of an exploration well, sandstones of the Exter Formation were hydraulically tested to determine the hydraulic properties and to evaluate chemical and microbial processes caused by drilling and water production. The aim was to determine the suitability of the formation as a reservoir for aquifer thermal energy storage. The tests revealed a hydraulic conductivity of 1–2 E-5 m/s of the reservoir, resulting in a productivity index of 0.6–1 m³/h/bar. A hydraulic connection of the Exter Formation to the overlaying, artesian “Rupelbasissand” cannot be excluded. Water samples were collected for chemical and microbiological analyses. The water was similarly composed as sea water with a maximum salinity of 24.9 g/L, dominated by NaCl (15.6 g/L Cl and 7.8 g/L Na). Until the end of the tests, the water was affected by drilling mud as indicated by the high pH (8.9) and high bicarbonate concentration (359 mg/L) that both resulted from the impact of sodium carbonate (Na₂CO₃) additives. The high amount of dissolved organic matter (>?58 mg/L) and its molecular-weight distribution pattern indicated that residues of cellulose, an ingredient of the drilling mud, were still present at the end of the tests. Clear evidence of this contamination gave the measured uranine that was added as a tracer into the drilling mud. During fluid production, the microbial community structure and abundance changed and correlated with the content of drilling mud. Eight taxa of sulfate-reducing bacteria, key organisms in processes like bio-corrosion and bio-clogging, were identified. It can be assumed that their activity will be affected during usage of the reservoir.  相似文献   

The chronostratigraphy of a quaternary sequence at the distal part of the nile littoral cell,Haifa Bay,Israel     

Simona Avnaim‐Katav  Ahuva Almogi‐Labin  Amir Sandler  Dorit Sivan  Naomi Porat  Ari Matmon 《第四纪科学杂志》2012,27(7):675-686

The current detailed chronostratigraphic framework of the last 1 Ma of an eastern Mediterranean sequence (Haifa Bay, Israel) aims to examine the relative roles of sea‐level changes, climate and tectonics. Seven continuous marine cores, up to ～120 m long, were recovered from shallow water depths. The cores were dated by optically stimulated luminescence, ¹⁴C, magnetostratigraphy, ²³⁰Th/²³⁴U, ²⁶Al/¹⁰Be, occurrence of index fossils and correlated to the global sea‐level curve and Marine Isotope Stages (MIS). The sedimentary sequence accumulated during the last ca. 1.0 Ma consists of 21 transgression–regression units with hiatuses between them. Five marine/terrestrial cycles, which occur in the lower part of the sequence, are attributed to the Jaramillo subchron and the Brunhes–Matuyama boundary, and correspond to MIS 29–21. The top ～50 m includes three sedimentary cycles deposited in the last ca. 400 ka. The regressive phases during this interval correspond to Glacial MIS 8, 6 and 2, while the transgressions correspond to Interglacial MIS 11, 7, 5 and 1. Thus, for the first time, this study documents the longest Quaternary succession dated so far in a key area of the Levant, sensitive to global history of sea‐level changes and glacial/interglacial fluctuations. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

Hydrodynamic and isotopic investigations for evaluating the mechanisms and amount of groundwater seepage through a rockslide dam     

Marco Petitta  Gabriele Scarascia Mugnozza  Maurizio Barbieri  Gianluca Bianchi Fasani  Carlo Esposito 《水文研究》2010,24(24):3510-3520

This study addresses the influence of landslide dams on surface water drainage and groundwater flow. In the study area of Scanno Lake and Sagittario River (Central Italy), a limestone rockslide‐avalanche formed a lake, which has an outlet that is occasionally active, showing infiltration into the rockslide dam. Several springs are present at the lake's base and are partly fed by seepage through the rockslide debris. Piezometric surveys, discharge measurements, pumping tests and chemical analyses are tools used to build a conceptual model of the groundwater flow and to evaluate the flow through the rockslide debris. Seasonal water isotopic signatures validate the assumed model, showing a mixing of infiltration recharge and groundwater seepage throughout the rockslide debris. Various recharge areas have been found for springs, pointing out those directly fed by the rockslide debris aquifer. Hypotheses about seasonal groundwater mixing between the regional carbonate aquifer and the rockslide debris aquifer are supported by isotope results. Seasonal changes in groundwater table level due to recharge and surface losses from seasonal outlet have been correlated with isotopic groundwater composition from the rockslide debris aquifer and the downstream springs; this relationship highlights the role of the rockslide dam body on the hydrodynamics of the studied area. Relationships between surface waters and groundwater in the area have been completely understood on the basis of water isotopic fingerprinting, finally obtaining a complete evaluation of groundwater renewable resources and its regimen. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献