Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

  相似文献   

Microwave Type III Burst Group in the 1993 October 2 Flare     

Qijun Fu  Zhihai Qin  Yuying Liu  Guangli Huang 《Astrophysics and Space Science》1997,250(2):189-204

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.  相似文献   

富氏谱分析法模拟地壳垂直运动的地学依据及其策应力模型     

张勤  范一中 《大地测量与地球动力学》2001,21(4):76-80

应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型  相似文献   

The Statistical Analysis of Migration of Strong Earthquakes-Taking the North China Region as an Example     

Qin Chengzhi 《中国地震研究》2004,18(2):178-187

The migration of strong earthquakes is an important research topic because the migration phenomena reflect partly the seismic mechanism and involve the prediction of tendency of seismic activity. Research on migration of strong earthquakes has mostly focused on finding the phenomena. Some attempts on getting regularity were comparatively subjective. This paper suggests that there should be indices of migration in earthquake dataset and the indexes should have statistical meaning if there is regularity in the migration of strong earthquakes. In this study, three derivative attributes of migration, i.e., migration orientation, migration distance and migration time interval, were statistically analyzed. Results in the North China region show that the migration of strong earthquakes has statistical meaning. There is a dominant migration orientation (W by S to E by N), a dominant distance (≤100km and on the confines of 300～700km), and a dominant time interval (≤1a and on the confines of 3～4a). The results also show that the migration will differ slightly with different magnitude range or earthquake activity phase.  相似文献   

星际A型CH_3CH－H_2含超精细能级的碰撞跃迁速率系数的理论计算     

裴春传  曾琴 《天文学报》1995,(1)

本文作者利用ＩＯＳ近似模型，计算了星际分子云条件下Ａ型ＣＨ３ＣＮ－Ｈ２含超精细能级的碰撞跃迁速率系数。温度范围是２０Ｋ－１４０Ｋ。为研究分子云与恒星形成区的物理、化学性质提供了有用的基础分子数据。  相似文献   

Automated Separation of Stars and Normal Galaxies Based on Statistical Mixture Modeling with RBF Neural Networks     

Dong-Mei Qin  Ping Guo  Zhan-Yi Hu  Yong-Heng ZhaoNational Laboratory of Pattern Recognition Laboratory  Institute of Automation  Chinese Academy of Sciences  Beijing  dmqin@nlpr.ia.ac.cnDepartment of Computer Sciences  Beijing Normal University  Beijing National Astronomical Observatories  Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2003,3(3)

For LAMOST, the largest sky survey program in China, the solution of the problem of automatic discrimination of stars from galaxies by spectra has shown that the results of the PSF test can be significantly refined. However, the problem is made worse when the redshifts of galaxies are not available. We present a new automatic method of star/(normal) galaxy separation, which is based on Statistical Mixture Modeling with Radial Basis Function Neural Networks (SMM-RBFNN). This work is a continuation of our previous one, where active and non-active celestial objects were successfully segregated. By combining the method in this paper and the previous one, stars can now be effectively separated from galaxies and AGNs by their spectra-a major goal of LAMOST, and an indispensable step in any automatic spectrum classification system. In our work, the training set includes standard stellar spectra from Jacoby's spectrum library and simulated galaxy spectra of EO, SO, Sa, Sb types with redshift ranging from 0 to 1  相似文献   

BTOPMC模型与新安江模型在史河上游的应用比较研究   总被引：1，自引：0，他引：1  

许钦  任立良  杨邦  刘晓帆 《水文》2008,28(2):23-25

介绍了一个由TOPMODEL发展而来的基于栅格的分布式水文模型--BTOPMC模型.选取史河流域黄泥庄水文站以上集水面积为研究区域,在空间分辨率为30秒的数字高程模型数据基础上构建数字流域水系,基于IGBP全球陆面土地覆被数据以及FAO全球数字土壤数据运用BTOPMC模型和新安江模型分别对黄泥庄站1982年到1987年的日径流过程进行模拟.采用模拟与实测洪量比和确定性系数对两个模型的模拟结果进行比较分析.结果表明两个模型都能很好地模拟日径流过程,新安江模型有更好的模拟效果.  相似文献   

Centrifugal test of a road embankment built after new dredger fill on thick marine clay     

Fayun Liang  Mingyi Zhao  Chengrui Qin  Yajie Jia  Zhongwei Wang  Guiping Yue 《Marine Georesources & Geotechnology》2020,38(1):114-121

Abstract

The construction of a 100-km road network is planned on a land reclamation area in the Oujiang Estuary in China. The embankment had a height of 4 m and a base width of 60 m. The reclamation area is newly filled by a 3-m dredger fill on a 48-m thick layer of marine clay. Estimation of the settlement of the future road network is difficult. To guide the construction of the road network, a 1/100-scale centrifuge model test was performed with a marine clay sample from the construction site to simulate the layered settlements and evaluate the drainage effect of prefabricated vertical drains in the dredger fill in the following 10 years. The results of the centrifuge modeling test are verified by 10-month in situ monitoring, which shows agreement between the centrifuge modeling test results and the in situ results. The test results indicate that additional time is needed to reinforce the newly added dredger fill by the surcharge preloading method to uplift the elevation of the reclamation area with dredger fill.  相似文献