Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes     

Kim IC  Kim YJ  Yoon YD  Kawamura S  Lee YS  Lee JS 《Marine environmental research》2004,58(2-5):125-129

To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.  相似文献   

Changing anthropogenic influence on the Santa Monica Bay watershed     

Dojiri M  Yamaguchi M  Weisberg SB  Lee HJ 《Marine environmental research》2003,56(1-2):1-14

Santa Monica Bay is an open coastal embayment located directly seaward of Los Angeles, California. The Bay provides vital economic value through its water-dependent activities, such as swimming, diving, boating, and fishing. An increase from 100,000 residents in 1900 to 10 million in 2000 has imposed numerous environmental stressors on the Bay, including urbanization of the watershed. Pollutant discharges into the Bay increased throughout the early part of the century, but declined following passage of the Clean Water Act in 1972. Since that time, the predominant source of pollutant inputs has changed from point sources to non-point urban runoff. To assess how present-day and historical pollution interact to affect the environmental quality of Santa Monica Bay, three organizations collaborated on a multi-disciplinary study in 1997, towards which this volume is focused. This paper details the temporal patterns of anthropogenic influence on Santa Monica Bay to provide context for the papers that follow.  相似文献   

Origin of the hydrocarbon component of carbonaceous chondrites: the star-meteorite connection     

Lee W  Wdowiak TJ 《The Astrophysical journal》1993,417(1):L49-L51

We report the results of an experiment that produced a residue which closely matches the hydrocarbon component of the Murchison carbonaceous chondrite. This experiment suggests that the parent material of the meteoritic component originated as polycyclic aromatic hydrocarbon species in carbon stars during their later stages of evolution. The experiments also indicate that the pathway from those formation sites to eventual incorporation into the meteorite parent body involved hydrogenation in a plasma in the solar nebula or in H II regions prior to the solar nebula. This model is consistent with what is known about the meteoritic hydrocarbon component including deuterium abundance, the observation of cosmic infrared emission bands best attributed to polycyclic aromatic hydrocarbon molecules, and the inherent stability of these molecules that allows their formation in stars and subsequent survival in the interstellar medium.  相似文献   

Plasma processing of interstellar PAHs into solar system kerogen     

Wdowiak TJ  Lee W  Cronin J  Beegle LW  Robinson MS 《Planetary and Space Science》1995,43(10-11):1175-1182

Processes resulting in the formation of hydrocarbons of carbonaceous chondrites and the identity of the interstellar molecular precursors involved are an objective of investigations into the origin of the solar system and perhaps even life on earth. We have combined the resources and experience of an astronomer and physicists doing laboratory simulations with those of a chemical expert in the analysis of meteoritic hydrocarbons, in a project that investigated the conversion of polycyclic aromatic hydrocarbons (PAHs) formed in stellar atmospheres into alkanes found in meteorites. Plasma hydrogenation has been found in the University of Alabama at Birmingham Astrophysics Laboratory to produce from the precursor PAH naphthalene, a new material having an IR absorption spectrum (Lee, W. and Wdowiak, T.J., Astrophys. J. 417, L49-L51, 1993) remarkably similar to that obtained at Arizona State University of the benzene-methanol extract of the Murchison meteorite (Cronin, J.R. and Pizzarello, S., Geochim. Cosmochim. Acta 54, 2859-2868, 1990). There are astrophysical and meteoritic arguments for PAH species from extra-solar sources being incorporated into the solar nebula, where plasma hydrogenation is highly plausible. Conversion of PAHs into alkanes could also have occurred in the interstellar medium. The synthesis of laboratory analogs of meteoritic hydrocarbons through plasma hydrogenation of PAH species is underway, as is chemical analysis of those analogs. The objective is to clarify this heretofore uninvestigated process and to understand its role during the origin of the solar system as a mechanism of production of hydrocarbon species now found in meteorites. Results have been obtained in the form of time-of-flight spectroscopy and chemical analysis of the lab analog prepared from naphthalene.  相似文献   

Mitigation of municipal solid waste leachate contamination at Payatas, Metro Manila, Philippines： A technical proposal     

Marvin Joseph Montefrio Kai Scene Ng Lee Yih Koh Erwin Torio Mark Hansley Chua Louernie de Sales-Papa Jeffrey Philip Obbard 《中国地球化学学报》2006,25(B08):122-122

The gross environmental condition of the Payatas Disposal Facility in Metro Manila, Philippines, implores the need for sustainable technologies to control and abate toxic municipal solid waste leachate and protect public health. A team of environmental engineers recognized this need by selecting the site as a design project for the Mondialogo Worldwide Engineering Award, sponsored by the United Nations and Daimler Chrysler. The project culminated in a technical proposal that highlighted the following design features： an impermeable dumpsite cap to control leachate generation; vertical impermeable barriers for containment and isolation of leachate; a drainage system to divert clean, surface water from the cap and prevent its contamination prior to discharge; a leachate recirculation system to enhance stabilisation of the waste deposit; and a facultative lagoon and constructed wetland system to treat excess leachate.  相似文献   

Analysis of the effect of geology, soil properties, and land use on groundwater quality using multivariate statistical and GIS methods     

Jin-Soo Lee Kyung-Seok Ko Tong-Kwon Kim Jae Gon Kim Seong-Hyun Cho In-Suk Oh 《中国地球化学学报》2006,25(B08):152-152

The regional survey of groundwater used as a small water supply system was performed to know the effect of geology, soil properties and land use on groundwater quality at Nonsan City, Korea. A total of 126 groundwater samples were collected and analyzed at the study area. The multivariate statistical methods, principal components analysis and discriminant analysis, and GIS technique were used for the quantitative interpretation of groundwater quality. The study area is mainly composed of Precambrian gneiss, Jurassic granite, and Cretaceous volcanics, and metasedimentary rocks of the Ogcheon zone. The land use was grouped as paddy, upland, grassland, resident, point source, industrial area, and water system. The soil properties were classified as 4 major groups, Entisols, Alfisols, Inceptisols, and Ultisols, by the degree of development, and reclassified as 11 subgroups. The modified and simplified geologic map, soil map, and land use map were made by using ARCGIS soft-ware. The area of geology, soil property, and land use affecting the groundwater quality for each well were also calculated by ARCGIS soft-ware to acquire the quantitative parameters for multivariate statistical analysis. The monitoring results of groundwater in the study area showed that 13%-21% of the groundwater samples exceeded the portable water guideline and the main causes were turbidity, bacteria, arsenic and nitrate-N. The spatial distribution of each component showed the close relationship between groundwater quality and geology reflecting the topography, land use.  相似文献   

地面地震层析技术在金属矿勘查中的试验研究   总被引：6，自引：0，他引：6  

徐明才  高景华  荣立新  柴铭涛  刘建勋  吕庆田  史大年 《物探与化探》2005,29(4):299-303

由于金属矿区地质构造的复杂性和复杂的地表条件, 适合于探测层状介质的地震反射方法技术在金属矿勘查中的应用受到了限制。为提高地震记录的横向分辨率, 探测复杂的地质构造及隐伏岩体等, 需研究相应的地震方法技术, 地面地震层析技术在金属矿勘查中的试验研究就是为解决上述问题而开展的。本文就非线性地面地震层析技术的基本原理进行了阐述, 并就该方法在金属矿勘查中的试验效果进行了讨论。  相似文献   

地下圆形衬砌隧道对沿线地震动的影响(II):数值结果   总被引：5，自引：3，他引：5  

梁建文  纪晓东  Vincent W Lee 《岩土力学》2005,26(5):687-692

利用地下圆形衬砌隧道对入射平面P波和SV波散射级数解答，定量分析了入射波长、入射角度、隧道直径、衬砌刚度等因素对沿线地表位移放大作用的影响。计算结果表明，当入射频率较低时，地下隧道的存在对地表位移幅值影响不大；而当入射频率较高时，地表位移幅值可以达到无隧道情况的4．5倍以上；衬砌情况下地表位移幅值可以高出非衬砌情况的87．8％。建议在地铁的规划设计中考虑地下隧道的建设对隧道沿线设计地震动的影响。  相似文献   

第四系沉积地层内剪切波分裂：新马德里地震带中部的新构造含意     

Harr.  JB 徐明才 《物探化探译丛》1997,(2):26-35

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任意圆弧形凸起地形中隧洞对入射平面SH波的影响   总被引：10，自引：2，他引：10       下载免费PDF全文

梁建文  罗昊  Vincent W.Lee 《地震学报》2004,26(5):495-508

借助辅助函数的思想， 采用波函数展开法给出了圆弧形凸起地形中隧洞对入射平面SH波影响问题的一个解析解．数值结果表明，凸起地形中隧洞的存在以及隧洞大小，对凸起地形表面运动，以及凸起地形中的隧洞的动应力集中均具有显著影响．   相似文献