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The aim of this study was to investigate the potential application of the beta-d-glucuronidase (GLUase) activity measurement for the routine detection and quantification of E. coli in marine bathing waters. GLUase activity was measured as the rate of hydrolysis of 4-methylumbelliferyl-beta-d-glucuronide. Culturable E. coli were quantified by the most probable number (MPN) microplate method. Both methods were applied to a large set of seawater samples. Significant correlation was found between the log of GLUase activity and the log of culturable E. coli. The mean coefficient of variation (CV) of the GLUase activity was less than 15% at concentrations around the current standards of International regulations whereas the CV of the microplate method was around 30%. When samples were stored at 4 degrees C and 20 degrees C, the mean CV of the GLUase activity remained below 15% up to 6 hours after sample collection whereas the range of variation of the microplate method varied between 10 and 50%. We concluded that the GLUase activity is an operational, reproducible, simple, very rapid and low cost method for the real-time enumeration of E. coli in bathing waters and should be preferred to the microplate method. The GLUase activity method should be routinely applied to the rapid enumeration of E. coli in recreational waters and recommendations for its application were suggested to water quality managers. 相似文献
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Santos RG Martins AS Farias Jda N Horta PA Pinheiro HT Torezani E Baptistotte C Seminoff JA Balazs GH Work TM 《Marine pollution bulletin》2011,62(6):1297-1302
To show the influence of coastal habitat degradation on the availability of food for green turtles (Chelonia mydas), we assessed the dietary preferences and macroalgae community at a feeding area in a highly urbanized region. The area showed low species richness and was classified as degraded. We examined stomach contents of 15 dead stranded turtles (CCL = 44.0 cm (SD 6.7 cm)). The diet was composed primarily of green algae Ulva spp. (83.6%). In contrast, the macroalgae community was dominated by the green alga Caulerpa mexicana. We found a selection for red algae, seagrass and Ulva spp., and avoidance for C. mexicana and brown alga Dictyopteris delicatula. The low diversity of available food items, possibly a result of environmental degradation, likely contributed to the low dietary diversity. The nutritional implications of this restricted diet are unclear. 相似文献
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This note describes the use of Microsoft Excel macros (programs written in Excel's internal language, Visual Basic for Applications) to create simple onscreen animations of transient ground water data within Excel. Compared to many specialized visualization software packages, the use of Excel macros is much cheaper, much simpler, and can rapidly be learned. The Excel macro can also be used to create individual GIF files for each animation frame. This series of frames can then be used to create an AVI video file using any of a number of graphics packages, such as Corel PhotoPaint. The technique is demonstrated through a macro that animates changes in the elevation of a water table along a transect over several years. 相似文献