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排序方式: 共有328条查询结果,搜索用时 15 毫秒
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现代腕足动物壳体的δ~(18)O和δ~(13)C值J。C。Scott等在过去的34年里,许多科技工作者利用古代腕足动物壳体的δ18值作为古海水δ18O值或温度的长期变化的指示物。最近,腕足动物壳体的δ18O值被用来计算古海水的温度、密度和盐度,以了解古... 相似文献
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Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 g/ml, but dropped off very sharply at higher protein concentrations. The apparent Km for 3-OH-BaP was 1.71 +/- 0.04 microM, and Vmax 1.26 +/- 0.16 nmol/min/mg protein (mean +/- SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4'-OH-CB72) and a model hexachloro-OH-PCB (4'-OH-CB159) were assayed with [14-C]-UDPGA and separation of the [14-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [14-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4'-OH-CB72 (Km 7.3 microM; Vmax 1.55 nmol/min/mg) than 4'-OH-CB159 (Km 16.1 microM; Vmax 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver. 相似文献
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The purpose of this research was to demonstrate a concentration effect of zinc exposure at organismal, cellular and sub-cellular levels in the eastern oyster and to find associated protein expression signatures (PES) for each concentration of zinc. Oysters were exposed to six concentrations of zinc for 48 h in a controlled environment. At the organismal level, fecal material was observed as a measure of physiological health during metal exposures. At the cellular level, lysosomal destabilization was measured using hemolymph. This cellular response was significant only at the highest concentration, when the fecal index was lowest. Protein responses were monitored in the oyster following exposure to zinc. Gill tissue was excised and homogenized, and then analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and digital image analysis. Protein expression signatures were found to be specific to each concentration. The protein responses were linked to the other biological parameters measured, each of which followed a concentration gradient of zinc. 相似文献
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利用雷电定位系统进行电网雷击故障快速查询(英文) 总被引:1,自引:0,他引:1
At present, lightning is one of the 10 natural disasters, and it is also the top environmental factor of power interruption. It often causes huge losses to the electric system. The Wuhan High Voltage Institute of the State Grid Corporation of China and Huazhong University of Science and Technology have been researching and developing lightning location systems (LLSs) since the late 1980s. In the mid-1990s, a lightning detection network was created in 29 provinces and cities in China. It is primarily applied to rapidly find lightning accidents, which greatly reduces power interruption. Also, it ensures high efficiency and safe operation of the electricity system. Remarkable benefit is achieved. China's LLS went through an "orientation positioning - time difference positioning - integrated positioning" development process. The positioning precision, detection efficiency, degree of automation, practicability and applied range are improved. Also, a lightning information system plan of the national network has been implemented, which services the whole society. 相似文献
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