Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)     

Blum JL  Knoebl I  Larkin P  Kroll KJ  Denslow ND 《Marine environmental research》2004,58(2-5):565-569

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.  相似文献   

Polycyclic aromatic hydrocarbons (PAHs) in Antarctic Martian meteorites, carbonaceous chondrites, and polar ice     

Becker L  Glavin DP  Bada JL 《Geochimica et cosmochimica acta》1997,61(2):475-481

Recent analyses of the carbonate globules present in the Martian meteorite ALH84001 have detected polycyclic aromatic hydrocarbons (PAHs) at the ppm level (McKay et al., 1996). The distribution of PAHs observed in ALH84001 was interpreted as being inconsistent with a terrestrial origin and were claimed to be indigenous to the meteorite, perhaps derived from an ancient martian biota. We have examined PAHs in the Antarctic shergottite EETA79001, which is also considered to be from Mars, as well as several Antarctic carbonaceous chondrites. We have found that many of the same PAHs detected in the ALH84001 carbonate globules are present in Antarctic carbonaceous chondrites and in both the matrix and carbonate (druse) component of EETA79001. We also investigated PAHs in polar ice and found that carbonate is an effective scavenger of PAHs in ice meltwater. Moreover, the distribution of PAHs in the carbonate extract of Antarctic Allan Hills ice is remarkably similar to that found in both EETA79001 and ALH84001. The reported presence of L-amino acids of apparent terrestrial origin in the EETA79001 druse material (McDonald and Bada, 1995) suggests that this meteorite is contaminated with terrestrial organics probably derived from Antarctic ice meltwater that had percolated through the meteorite. Our data suggests that the PAHs observed in both ALH84001 and EETA79001 are derived from either the exogenous delivery of organics to Mars or extraterrestrial and terrestrial PAHs present in the ice meltwater or, more likely, from a mixture of these sources. It would appear that PAHs are not useful biomarkers in the search for extinct or extant life on Mars.  相似文献   

A search for endogenous amino acids in the Martian meteorite EETA79001     

McDonald GD  Bada JL 《Geochimica et cosmochimica acta》1995,59(6):1179-1184

The Antarctic shergottite EETA79001 is believed to be an impact-ejected fragment of the planet Mars. Samples of the carbonate (white druse) and the basaltic (lithology A) components from this meteorite have been found to contain amino acids at a level of approximately 1 ppm and 0.4 ppm, respectively. The detected amino acids consist almost exclusively of the L-enantiomers of the amino acids commonly found in proteins, and are thus terrestrial contaminants. There is no indication of the presence of alpha-aminoisobutyric acid, one of the most abundant amino acids in several carbonaceous chondrites. The relative abundances of amino acids in the druse material resemble those in Antarctic ice, suggesting that the source of the amino acids may be ice meltwater. The level of amino acids in EETA79001 druse is not by itself sufficient to account for the 600-700 ppm of volatile C reported in druse samples and suggested to be from endogenous martian organic material. However, estimates of total terrestrial organic C present in the druse material based on our amino acid analyses and the organic C content of polar ice can account for most of the reported putative organic C in EETA79001 druse.  相似文献   

碧口群火山岩的时代——SHRIMP锆石U—Pb测年结果   总被引：12，自引：0，他引：12  

闫全人  王宗起闫臻 AndrewD.Hanson PeterA.Druschke刘敦一  宋彪简平 王涛 《地质通报》2003,22(6):456-458

碧口群火山岩系是从原碧口系(叶连俊等,1944,后称碧口群)中解体出来的一个岩石单元(陶洪祥等,1993)。虽经过近60年的研究,但对某些关键问题,特别是碧口群火山岩系的时代,仍存在不同认识。在早期研究阶段(20世纪40～50年代)和60～70年代的区域地质调查工作中,碧口群和横丹群被视做一个整体,并通过地层层序关系探讨其时代。如,叶连俊等(1944)提出碧口系形成于志留—泥盆纪,黄振辉(1959)则认为碧口群属寒武—志留系。80年代以来对碧口群火山岩系进行的同位素年代学和地古生物学研究给出了从新元古代(赵祥生等,1990;胡正东,1990;李耀敏,1991;秦…  相似文献   

蛇绿岩及蛇绿岩中浅色岩的SHRIMP U-Pb测年   总被引：101，自引：2，他引：101  

简平  刘敦一  张旗  张福勤  石玉若  施光海  张履桥  陶华 《地学前缘》2003,10(4):439-456

文中简要评述了蛇绿岩的层状辉长岩,斜长岩和斜长花岗岩,以橄榄岩为主岩的花岗岩和蛇绿岩中的埃达克岩的锆石SHRIMP U-Pb年龄的地质意义。层状辉长岩(或堆晶层状辉长岩)通常起源于洋脊下的岩浆房,因而它的形成年龄代表洋壳形成的时代。斜长岩与层状辉长岩的时代相近或略晚。斜长花岗岩年龄的解释极其依赖锆石组成和地球化学证据。橄榄岩为主岩的花岗岩,可能记录蛇绿岩的侵位时代。蛇绿岩中的埃达克岩是消减洋壳在深部的部分熔融的产物。文中发表了新疆扎河坝蛇绿岩SHRIMP定年的中间成果,并简略地介绍了滇川西部金沙江和内蒙古图林凯等地的研究实例。根据层状辉长岩的测定结果,扎河坝蛇绿岩形成于(489±4)Ma,金沙江蛇绿岩形成于(328±8)Ma。内蒙古图林凯蛇绿岩中埃达克岩形成于(467±13)Ma～(429±7)Ma。块状辉长岩、斜长花岗岩和橄榄岩为主岩的花岗质岩石记录了蛇绿岩的复杂演化。新疆扎河坝蛇绿岩中的块状辉长岩中存在多组锆石年龄值。较老的一组为468～511 Ma,与层状辉长岩和斜长岩相似,记录了蛇绿岩或洋壳的形成时代,但是,岩石中的大部分锆石年龄为396～419 Ma,加权平均年龄为(406±4)Ma,可能反映了一次部分熔融事件。滇川西部金沙江蛇绿岩中的斜长花岗岩的形成年龄为约300～285Ma,晚于层状辉长岩和?  相似文献   

滇川西部金沙江石炭纪蛇绿岩SHRIMP测年:古特提斯洋壳演化的同位素年代学制约   总被引：88，自引：10，他引：88       下载免费PDF全文

简平  刘敦一  孙晓猛 《地质学报》2003,77(2):217-228

本文应用SHRIMP方法精确测定了金沙江蛇绿岩带中蛇绿岩的辉长岩和斜长岩、呈脉状产于辉长岩和变质橄橄岩中的斜长花岗岩、以及呈岩株状侵入蛇绿岩中的花岗闪长岩的锆石U-Pb年龄,提供了古特提斯洋壳演化的年代学制约。滇西之用层状角闪辉长岩的年龄为328±8Ma,书松斜长岩为329±7Ma,白马雪山辉长岩为282～285Ma,它们可能反映了海底扩张不同阶段的时代。研究还表明,在这些辉长岩和斜长岩中,部分锆石记录了375～352Ma的略老的年龄,暗示蛇绿岩浆活动可能始于晚泥盆世。在金沙江蛇绿岩中,滇西娘九丁斜长花岗岩和川西雪堆斜长花岗岩,具有高硅低钾的成分特征。但是,这些岩石的REE总量高,LREE富集;Sr初始值高,达0.7058～0.7070;在其锆石组成中,存在继承锆石。这些证据表明斜长花岗岩中存在陆壳物质的混染,可能与洋壳俯冲消减有关。娘九丁斜长花岗岩的年龄为285±6Ma,雪堆斜长花岗岩为300±5Ma,记录了古特提斯洋壳俯冲消减事件。滇西吉义独花岗闪长岩,呈岩株状侵入蛇绿岩中,年龄为263±6Ma,限定了蛇绿混杂岩的年代上限。  相似文献   

构造年代学——当今构造研究的一个新学科   总被引：10，自引：1，他引：10  

杨巍然  简平 《地质科技情报》1996,15(4):39-43

以大别山高压超高压变质带构造年代学研究为例阐述了构造年代学。它是90年代以来构造地质学的一个新的分支学科。构造年代学研究内容和方法为：进行区域构造分析和典型地区构造解析；根据具体地质构造情况和研究目标的要求选择同位素年代测试方法和进行同位素年代的取样、选样和测试；通过综合分析和对比建立构造年代学时空格局。  相似文献   

大别山西部熊店加里东期榴辉岩——同位素地质年代学的证据   总被引：19，自引：6，他引：19       下载免费PDF全文

简平  杨巍然  李志昌  周慧芳 《地质学报》1997,71(2)

以UP-Pb单颗粒锆石同位素稀释法、角闪石~(40)Ar—~(39)Ar坪年龄和Sm-Nd全岩及矿物等时线相结合的研究方法,系统地研究了大别造山带西部熊店榴辉岩的峰变质年龄,可能的原岩年龄和退变质年龄,建立了这一榴辉岩的构造-变质时间序列,提出了大别地区存在加里东期榴辉岩的确凿证据。  相似文献   

湖北蕲春花岗岩类锆石SHRIMP年龄：大别山造山带内弱变质—未变质晋宁期花岗岩类的发现   总被引：2，自引：0，他引：2       下载免费PDF全文

薛怀民  刘敦一  董树文  简平  刘晓春 《地质学报》2004,78(1):81-88

蕲春花岗质杂岩体包括斑状二长花岗岩和花岗岩两部分,它们之间在化学性质上存在着很大的差异,前者表现为高Al 2O3(15.73%)、相对高CaO(2.46%)、Na2O含量明显高于K2O(Na2O/K2O=1.27),尤以强烈亏损重稀土元素和极强的轻、重稀土元素分馏程度[(La/Yb)N=46.8]为特征而类似于太古宙高Al2O3的TTG岩石.而后者则以较低的Al2O3含量(14.05%)、贫CaO(0.82%)、K2O含量明显高于Na2O(Na2O/K2O=0.81)为特征,轻、重稀土元素的分馏程度[(La/Yb)N=10.89]也较片麻状二长花岗岩中弱得多.两类岩石中锆石的U-PbSHRIMP年龄分别为824.6±17.6 Ma和784±20 Ma,该时代与大别山造山带内花岗片麻岩的原岩形成年龄类似.大别山造山带内弱变质-未变质晋宁期花岗岩的出现表明扬子板块印支期向北俯冲时,该花岗质杂岩处于俯冲板片的后缘,可代表造山带内扬子基底的原地露头.而岩体周围的高压变质杂岩应是折返上来的无根构造岩片,大别山造山带内高压超高压变质杂岩的出露不是整体性抬升剥蚀的结果.  相似文献   

湖北新春花岗岩类锆石SHRIMP年龄:大别山造山带内弱变质—未变质晋宁期花岗岩类的发现     

薛怀民  刘敦一  董树文  简平  刘晓春 《地质学报》2004,78(1)

蕲春花岗质杂岩体包括斑状二长花岗岩和花岗岩两部分,它们之间在化学性质上存在着很大的差异,前者表现为高Al_2O_3(15.73％)、相对高CaO(2.46％)、Na_2O含量明显高于K_2O(Na_2O/K_2O=1.27),尤以强烈亏损重稀土元素和极强的轻、重稀土元素分馏程度[(La/Yb)_N=46.8]为特征而类似于太古宙高Al_2O_3的TTG岩石。而后者则以较低的Al_2O_3含量(14.05％)、贫CaO(0.82％)、K_2O含量明显高于Na_2O(Na_2O/K_2O=0.81)为特征,轻、重稀土元素的分馏程度[(La/Yb)_N=10.89]也较片麻状二长花岗岩中弱得多。两类岩石中锆石的U-PbSHRIMP年龄分别为824.6±17.6 Ma和784±20 Ma,该时代与大别山造山带内花岗片麻岩的原岩形成年龄类似。大别山造山带内弱变质-未变质晋宁期花岗岩的出现表明扬子板块印支期向北俯冲时,该花岗质杂岩处于俯冲板片的后缘,可代表造山带内扬子基底的原地露头。而岩体周围的高压变质杂岩应是折返上来的无根构造岩片,大别山造山带内高压-超高压变质杂岩的出露不是整体性抬升剥蚀的结果。  相似文献