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The measurement of enzymic activity in plankton communities is useful in the study of marine ecosystems. Such measurements can lead to a clearer understanding of the biological transformations in plankton communities at a particular time. However, the assays are somewhat time-consuming. To facilitate analysis of large numbers of samples, we have developed a method of quick-freezing of whole cells and of cell-free extracts of the diatom, Skeletonema costatum, followed by storage at ?60°C for up to one week. No loss in either the electron transport system (ETS) or the glutamate dehydrogenase (GDH) activity occurs. Similar conditions of storage can be used for the preservation of ETS activity in the marine copepod Calanus pacificus. Also, no measurable loss in either the GDH or the ETS activity is detectable after the quick-frozen whole cells of S. costatum have been kept frozen for over a year.  相似文献   
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Kenner  Robert  Gischig  Valentin  Gojcic  Zan  Qu&#;au  Yvain  Kienholz  Christian  Figi  Daniel  Th&#;ny  Reto  Bonanomi  Yves 《Landslides》2022,19(6):1357-1377
Landslides - Lidar measurements and UAV photogrammetry provide high-resolution point clouds well suited for the investigation of slope deformations. Today, however, the information contained in...  相似文献   
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Snow cover influences the thermal regime and stability of frozen rock walls. In this study, we investigate and model the impact of the spatially variable snow cover on the thermal regime of steep permafrost rock walls. This is necessary for a more detailed understanding of the thermal and mechanical processes causing changes in rock temperature and in the ice and water contents of frozen rock, which possibly lead to rock wall instability. To assess the temporal and spatial evolution and influence of the snow, detailed measurements have been carried out at two selected points in steep north‐ and southfacing rock walls since 2012. In parallel, the one‐dimensional energy balance model SNOWPACK is used to simulate the effects of snow cover on the thermal regime of the rock walls. For this, a multi‐method approach with high temporal resolution is applied, combining meteorological, borehole rock temperature and terrain parameter measurements. To validate the results obtained for the ground thermal regime and the seasonally varying snowpack, the model output is compared with near‐surface rock temperature measurements and remote snow cover observations. No decrease of snow depth at slope angles up to 70° was observed in rough terrain due to micro‐topographic structures. Strong contrasts in rock temperatures between north‐ and south‐facing slopes are due to differences in solar radiation, slope angle and the timing and depth of the snow cover. SNOWPACK proved to be useful for modelling snow cover–rock interactions in smooth, homogenous rock slopes.  相似文献   
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In February 2014, a rock pillar with a volume of around 150 000  m3 collapsed at Piz Kesch in the Eastern Swiss Alps. A reconstruction of the conditions prior to the event and of the event itself is presented on the basis of different sources of data. The methods applied include photogrammetry, terrestrial laser scanning, structural geological analysis, examination of meteorological data, carbon‐14 (14C) dating of organic material in permafrost ice from a tension crack and numerical modelling of likely modes of failure. Despite a complete lack of in situ measurements in the rock wall prior to the event and of direct observations during the event, the available data allow the determination of the approximate timing of the event as well as the structural predisposition, the probable mode of failure and the timescale of several millennia involved in the triggering of the failure of the rock pillar. The interdisciplinary analysis of this event contributes towards understanding the complex interaction of processes involved in large rock slope failures currently occurring in warming mountain permafrost regions. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   
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Geological studies show evidence for temporal clustering of large earthquakes on individual fault systems. Since post-seismic deformation due to the inelastic rheology of the lithosphere may result in a variable loading rate on a fault throughout the interseismic period, it is reasonable to expect that the rheology of the non-seismogenic lower crust and mantle lithosphere may play a role in controlling earthquake recurrence times. We study this phenomenon using a 2-D, finite element method continuum model of the lithosphere containing a single strike-slip fault. This model builds on a previous study using a 1-D spring-dashpot-slider analogue of a single fault system to study the role of Maxwell viscoelastic relaxation in producing non-periodic earthquakes. In our 2-D model, the seismogenic portion of the fault slips when a predetermined yield stress is exceeded; stress accumulated on the seismogenic fault is shed to the viscoelastic layers below and recycled back to the seismogenic fault through viscoelastic relaxation. We find that random variation of the fault yield stress from one earthquake to the next can cause the earthquake sequence to be clustered; the amount of clustering depends on a non-dimensional number, W , called the Wallace number defined as the standard deviation of the randomly varied fault yield stress divided by the effective viscosity of the system times the tectonic loading rate. A new clustering metric based on the bimodal distribution of interseismic intervals allows us to investigate clustering behaviour of systems over a wide range of model parameters and those with multiple viscoelastic layers. For models with   W ≥ 1  clustering increases with increasing W , while those with   W ≤ 1  are unclustered, or quasi-periodic.  相似文献   
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