Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

LHRH-A和hCG对南方鲶血清促性腺激素水平的影响     

邓思平  王德寿  张耀光  吴天利 《广东海洋大学学报》2003,23(1):13-16

运用放射免疫分析法测定了促黄体释放激素类似物 (LHRH A)和人绒毛膜促性腺激素(hCG)对南方鲶血清促性腺激素 (GTH)水平的影响 ,结果表明 :LHRH A和hCG都能提高南方鲶一龄幼鱼血清GTH水平 ,hCG与LHRH A结合注射后血清GTH水平显著高于单独注射hCG或LHRH A后的血清GTH水平。LHRH A和hCG结合注射可诱导性成熟南方鲶排卵 ,同时排卵过程中伴随着血清GTH水平的急剧上升 ,在排卵后迅速下降 ;注射同样剂量的药物而未排卵的血清GTH水平虽有升高 ,但升高的幅度不及排卵鱼。这种血清GTH水平的急剧增高对诱导南方鲶卵母细胞最后成熟和排卵具有重要作用。  相似文献   

Optical flashes and very early afterglows in wind environments     

X. F. Wu  Z. G. Dai  Y. F. Huang  T. Lu 《Monthly notices of the Royal Astronomical Society》2003,342(4):1131-1138

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观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

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An approximate method for evaluating the shear band thickness in saturated soils     

X. B. Lu  Wang Shuyun  Wang Yihua  Peng Cui 《国际地质力学数值与分析法杂志》2004,28(15):1533-1541

A formula for the thickness of a shear band formed in saturated soils under a simple shear or a combined stress state has been proposed. It is shown that the shear band thickness is dependent on the pore pressure properties of the material and the dilatancy rate, but is independent of the details of the combined stress state. This is in accordance with some separate experimental observations. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

一种从经纬度网格资料获得正方形网格资料的插值方案     

邓爱军  卢敬华 《成都信息工程学院学报》1990,(Z1)

本文提出了一种把经纬度网格点上的气象要素值转化到正方形网格点上的多项式插值方案。对插值方案作了稳定性讨论和定量及定性分析。结果表明,这种插值方案方便可行,而且有广泛适用性。  相似文献   

An explicit representation of steady state response of a beam on an elastic foundation to moving harmonic line loads     

Lu Sun 《国际地质力学数值与分析法杂志》2003,27(1):69-84

A closed‐form deflection response of a beam rest is presented in this paper using the integral transform method. The theory of linear partial differential equations is used to represent the deflection of beam subjected to a moving harmonic line load in integration form. The solution is finally carried out using the inverse Fourier transform. To evaluate the integration analytically, poles of the integrand are identified with the help of algebraic equation theory. Residue theorem is then utilized to represent the integration as a contour integral in the complex plane. Closed‐form deflections and numerical results are provided for different combinations of load frequency and velocity. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

Approach to Mountain Hazards in Tibet, China   总被引：1，自引：1，他引：0  

MADongtao TUJianjun CUIPeng LURuren 《山地科学学报》2004,1(2):143-154

Tibet is located at the southwest boundary of China. It is the main body of the Qinghai-Tibet Plateau, the highest and the youngest plateau in the world. Owing to complicated geology, Neo-tectonic movements, geomorphology, climate and plateau environment, various mountain hazards, such as debris flow, flash flood, landslide, collapse, snow avalanche and snow drifts, are widely distributed along the Jinsha River (the upper reaches of the Yangtze River), the Nu River and the Lancang River in the east, and the Yarlungzangbo River, the Pumqu River and the Poiqu River in the south and southeast of Tibet. The distribution area of mountain hazards in Tibet is about 589,000 km^2, 49.3% of its total territory. In comparison to other mountain regions in China, mountain hazards in Tibet break out unexpectedly with tremendously large scale and endanger the traffic lines, cities and towns, farmland, grassland, mountain environment, and make more dangers to the neighboring countries, such as Nepal, India, Myanmar and Bhutan. To mitigate mountain hazards, some suggestions are proposed in this paper, such as strengthening scientific research, enhancing joint studies, hazards mitigation planning, hazards warning and forecasting, controlling the most disastrous hazards and forbidding unreasonable human exploring activities in mountain areas.  相似文献   

一次西南低涡过程的地面场中尺度分析     

曾俊  卢敬华  潘鄂芬 《成都信息工程学院学报》1990,(Z1)

本文运用带通滤波分析方法,对四川省1981年7月由西南低涡等天气系统引起的大暴雨过程作了地面气象要素场的中尺度诊断分析。结果表明,带通滤波后的场具有明显的中尺度特征,其中一些物理量场的适当配置与6小时后的暴雨中心有较好的对应关系。说明了此带通滤波方法在实际业务预报中具有一定的使用价值。  相似文献