Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae     

GUO Minrui  CHEN Guogang  CHEN Jiluan  ZHENG Minggang 《中国海洋大学学报(英文版)》2019,(5)

The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain fatty acid elongase, named Ns FAE, was cloned from Nannochloropsis sp.. The open reading frame of Ns FAE(GenBank accession no. MF680548) consisted of 1068 bp and encoded a predicted protein of 355 amino acids with molecular mass 38.8 k Da. The deduced polypeptide showed 43%–44% identity to fatty acyl elongases from other algae. RT-PCR experiments indicated that the Ns FAE gene exhibited the highest expression in Nannochloropsis sp. at 72 h(i.e., during the third growth stage) and the expression was significantly lower in the other four growth stages. Plasmid p Ns FAE-CRISPR and a recombinant DNA fragment(ADH1p-Ns FAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using the CRISPR-Cas system. Yeast transformants containing Ns FAE produced three fatty acids not normally present in wild-type BY4742-linoleic acid, linolenic acid and eicosadienoic acid-indicating that Ns FAE encodes a functional elongase enzyme.  相似文献   

香港世界地质公园晚侏罗世荔枝庄组岩石组合及其成岩地质过程解析     

姜杨  杨家明  杨祝良  沈加林  马雪  陈羽岚  钱迈平  邢光福  余明刚  段政 《地质论评》2016,62(3):759-769

中国东南大陆晚侏罗世地层普遍缺失,仅零星见于个别地区,香港新界东北的荔枝庄组即为其一。荔枝庄组出露于香港世界地质公园沉积岩园区的荔枝庄地区,自下往上由火山岩—沉积岩—火山岩组合而成,沉积岩中发育大型包卷层理和滑塌构造等典型沉积构造,是香港地区最具代表性的晚侏罗世火山—沉积岩系。通过实测地层剖面研究,确定其成岩过程大体上可划分为早期普林尼式火山爆发、中期破火山口湖相沉积和晚期普林尼式火山爆发三个阶段,以湖相沉积作用为主、火山喷发作用为辅;受晚期火山岩浆活动的影响,沉积岩层普遍发生硅化或炭化。荔枝庄组独特的岩石组合与形成的古地理环境,为探讨中国东南大陆中生代火山活动—沉积作用方式与成岩过程,提供了难得的研究实例。  相似文献   

吉林白山刘家堡子狼洞沟金银矿床地球化学特征及成因探讨          下载免费PDF全文

吴明刚  王胜炬  王贤孝 《山东国土资源》2016,32(4):17-21

通过对刘家堡子-狼洞沟金银矿床地质、成矿元素和同位素地球化学特征的分析和研究,发现矿区内近EW向和NE向断裂构造是主要容矿构造;最佳找矿元素前缘晕组合为As-Sb-Zn,矿上晕元素组合为Au-Ag-Pb-Hg,尾晕元素组合为Ni-Cu-Co;成矿作用中,古老基底中元古宙老岭群变质岩系为成矿提供了丰富的物质来源,而燕山期构造岩浆活动在提供成矿物质的同时还提供了热动力。刘家堡子-狼洞沟金银矿床应划属为与燕山晚期超浅成中酸性岩浆岩有关的中低温热液构造裂隙充填型金银矿床。  相似文献   

华南地区7-10月两类区域性极端降水事件特征及环流异常对比   总被引：2，自引：0，他引：2  

孙婧超  管兆勇  李明刚  于亚鑫 《气象学报》2019,77(1):43-57

利用1981—2016年7—10月中国753站逐日降水资料、气象信息综合分析处理系统（MICAPS）逐日站点降水资料、日本东京台风中心西北太平洋热带气旋（TC）最佳路径资料和NCEP/NCAR再分析资料集,分析了华南地区区域性日降水极端事件（RDPE事件）的统计特征及环流异常。根据华南地区RDPE事件的发生是否受热带气旋影响将其分为TCfree-RDPE和TCaff-RDPE两类事件,其中TCaff-RDPE事件占42%且集中发生在8月4—5候;TCfree-RDPE事件以7月发生频数最多,占其总频次的1/2以上。TCfree-RDPE事件发生时,华南地区受异常气旋性环流控制,来自西太平洋和中国南海的暖湿气流与北方冷气团在此汇合并形成一条狭长的水汽辐合带,低层辐合、高层辐散,显著强烈的上升运动为TCfree-RDPE事件的发生与维持提供了有利条件;与此同时,波扰动能量由高原东北侧及河西走廊地区向华南一带传播并在华南显著辐合,有利于华南上空扰动的发展和维持。TCaff-RDPE事件发生时,华南上空由低层到高层的斜压环流结构更为明显,异常上升运动更加强烈,热带气旋在其运动过程中携带了大量源自孟加拉湾、中国南海和西太平洋地区的水汽并输送至华南地区,水汽辐合气流更为强盛。同时,波扰动能量由高纬度地区沿河西走廊向下游传播,但在华南地区辐合不甚明显。两类极端事件发生时,加热场上的差异亦明显。华南及邻近地区上空的大气净加热及其南侧大范围区域的净冷却所形成的加热场梯度对TCfree-RDPE事件的发生有利。而TCaff-RDPE事件发生时,〈Q₁〉和〈Q₂〉在经向上由18°N以南、华南及其邻近地区、32°N以北呈负—正—负的异常分布型,正距平值更高,加热场梯度更大,有利于TCaff-RDPE事件的维持。这些结果有利于人们认识和预测华南区域性日降水极端事件的发生。   相似文献   

浙东南英安质火山岩早侏罗世锆石SHRIMP年龄的首获及其地质意义   总被引：12，自引：0，他引：12       下载免费PDF全文

陈荣邢光福  杨祝良周宇章余明刚  李龙明 《地质论评》2007,53(1):31-35

浙江中生代晚期火山岩极为发育，而有无中生代早期火山岩，则向无定论。本文在确认浙东南松阳毛弄煤矿一带煤系沉积下部火山岩属中生界毛弄组的基础上，测得其SHRIMP锆石U-Pb年龄为180±4Ma，首次证明浙江存在早一中侏罗世火山岩，同时表明浙东南尚没有确切的中侏罗世晚期至晚侏罗世火山岩活动记录，而且也缺失晚侏罗世和大部分中侏罗世地层。  相似文献   

浙江晚中生代雁荡山地貌成因及其与火山构造协同性演化规律     

段政  张翔  卢琴飞  陈荣  余明刚  洪文涛  褚平利 《地质学报》2022,96(6):2021-2038

浙江雁荡山是东南沿海一座典型的晚中生代破火山，以发育丰富的白垩纪流纹质火山岩地貌为特色，主要包括火山岩相剖面和火山岩地貌两种类型地质遗迹。本次通过详细的野外地质调查、岩石学研究及遥感图像分析，进一步将其中火山岩相剖面划分为溢流相、基底涌流相、火山碎屑流相、空落相剖面地质遗迹四小类；火山岩地貌分为叠嶂、锐峰和岩洞地貌地质遗迹三小类，并综合分析了其分布规律与成因演化过程。研究表明这些地质遗迹的分布和演化明显受到火山构造与火山岩相的控制。另外，研究区显著发育3级夷平面，反映其至少经历过3期隆升- 剥蚀事件，是太平洋板块、菲律宾海板块与欧亚大陆板块相互作用的动力学过程及印度板块碰撞远程效应的综合影响结果，同时其也是研究流纹质火山岩地貌成因与演化过程的理想基地。  相似文献   

空气动力学阻抗的测量研究          下载免费PDF全文

刘绍民 卢俐毛德发  胡明罡 《应用气象学报》2007,18(2):247-250

利用涡旋相关方法和蒸发皿方法分别测量了裸地和玉米田的空气动力学阻抗, 分析了空气动力学阻抗的日变化特征, 同时对两种测量方法进行了比较。结果表明:实测空气动力学阻抗的日变化比较明显, 但变化幅度不大 (0~200 s/m)。两种方法得到的空气动力学阻抗具有可比性, 但蒸发皿方法测量的空气动力学阻抗普遍小于涡旋相关方法的测量值。  相似文献   

全球海洋人为碳储量估算及时空分布研究进展     

Huang Peng  Chen Liqi  Cai Minggang 《地球科学进展》2015,30(8):952-959

海洋是全球最大的碳汇,确定海洋中人为碳含量及其时空变化是一件具有挑战性的工作。就当前海洋人为碳含量确定方法、人为碳储量及其随时间变化进行综述总结。研究表明海洋仍然可以吸收并储藏大量的人为碳,但未来对于海洋吸收储藏人为碳的研究仍具有诸多不确定性。已有证据表明稳态海洋的概念不完全正确,需要在非稳态条件下重新定义人为碳并开展研究。同时需要积累更多的观测数据,采用包括观测和模式模拟等手段来研究不同时间尺度的人为碳的吸收和储藏过程。  相似文献   

Two Periods of Skarn Mineralization in the Baizhangyan W–Mo Deposit,Southern Anhui Province,Southeast China: Evidence from Zircon U–Pb and Molybdenite Re–Os and Sulfur Isotope Data          下载免费PDF全文

Chunhai Li  Yaohui Jiang  Guangfu Xing  Kunyi Guo  Chao Li  Minggang Yu  Peng Zhao  Zheng Wang 《Resource Geology》2015,65(3):193-209

The recently discovered Baizhangyan skarn‐porphyry type W–Mo deposit in southern Anhui Province in SE China occurs near the Middle–Lower Yangtze Valley polymetallic metallogenic belt. The deposit is closely temporally‐spatially associated with the Mesozoic Qingyang granitic complex composed of g ranodiorite, monzonitic g ranite, and alkaline g ranite. Orebodies of the deposit occur as horizons, veins, and lenses within the limestones of Sinian Lantian Formation contacting with buried fine‐grained granite, and diorite dykes. There are two types of W mineralization: major skarn W–Mo mineralization and minor granite‐hosted disseminated Mo mineralization. Among skarn mineralization, mineral assemblages and cross‐cutting relationships within both skarn ores and intrusions reveal two distinct periods of mineralization, i.e. the first W–Au period related to the intrusion of diorite dykes, and the subsequent W–Mo period related to the intrusion of the fine‐grained granite. In this paper, we report new zircon U–Pb and molybdenite Re–Os ages with the aim of constraining the relationships among the monzonitic granite, fine‐grained granite, diorite dykes, and W mineralization. Zircons of the monzonitic granite, the fine‐grained granite, and diorite dykes yield weighted mean U–Pb ages of 129.0 ± 1.2 Ma, 135.34 ± 0.92 Ma and 145.3 ± 1.7 Ma, respectively. Ten molybdenite Re–Os age determinations yield an isochron age of 136.9 ± 4.5 Ma and a weighted mean age of 135.0 ± 1.2 Ma. The molybdenites have δ³⁴S values of 3.6‰–6.6‰ and their Re contents ranging from 7.23 ppm to 15.23 ppm. A second group of two molybdenite samples yield ages of 143.8 ± 2.1 and 146.3 ± 2.0 Ma, containing Re concentrations of 50.5–50.9 ppm, and with δ³⁴S values of 1.6‰–4.8‰. The molybdenites from these two distinct groups of samples contain moderate concentrations of Re (7.23–50.48 ppm), suggesting that metals within the deposit have a mixed crust–mantle provenance. Field observation and new age and isotope data obtained in this study indicate that the first diorite dyke‐related skarn W–Au mineralization took place in the Early Cretaceous peaking at 143.0–146.3 Ma, and was associated with a mixed crust–mantle system. The second fine‐grained granite‐related skarn W–Mo mineralization took place a little later at 135.0–136.9 Ma, and was crust‐dominated. The fine‐grained granite was not formed by fractionation of the Qingyang monzonitic granite. This finding suggests that the first period of skarn W–Au mineralization in the Baizhangyan deposit resulted from interaction between basaltic magmas derived from the upper lithospheric mantle and crustal material at 143.0–146.3 and the subsequent period of W–Mo mineralization derived from the crust at 135.0–136.9 Ma.  相似文献   

Carbon and Nitrogen Isotopic,and Mineral Inclusion Studies on the Diamonds from the Pozanti‐Karsanti Chromitite,Turkey     

DUAN Zheng  CHU Pingli  YU Minggang  ZHANG Xiang 《《地质学报》英文版》2019,93(Z2):16-17

He Pozanti‐Karsanti ophiolite (PKO) is one of the largest oceanic remnants in the Tauride belt, Turkey. Micro‐diamonds were recovered from the podiform chromitites, and these were investigated based on morphology, color, cathodoluminescence, nitrogen content, carbon and nitrogen isotopes, internal structure and inclusions. The diamonds recovered from the PKO are mainly mixed‐habit diamonds with sectors of different brightness under the cathodoluminescence images. The total δ¹³C range of the PKO diamonds ranges between ?18.8 ‰ and ?28.4 ‰, with a principle δ¹³C mode at ?25 ‰. Nitrogen contents of the diamonds range from 7 to 541 μg/g with a mean value of 171 μg/g, and the δ¹⁵N values range from ?19.1 ‰ to 16.6 ‰, with a δ¹⁵N mode of ?9 ‰. Stacking faults and partial dislocations are commonly observed in the Transmission Electron Microscopy foils whereas inclusions are rather rare. Combinations of (Ca_0.81Mn_0.19)SiO₃, NiMnCo‐alloy and nano‐size, quenched fluid phases were observed as inclusions in the PKO diamonds, confirming a natural origin of these diamonds. We believe that the δ¹³C‐depleted carbon signature of the PKO diamonds is a remnant of previously subducted crustal matter. These diamonds may have crystallized in metal‐rich melts in the asthenospheric mantle at depth below 250 km which were subsequently carried rapidly upward by asthenospheric melts/fliuds. We concluded that diamond‐bearing asthenospheric melts were likely involved in the formation of the Pozanti‐Karsanti podiform chromitite.  相似文献