Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene     

Telli-Karakoç F  Ruddock PJ  Bird DJ  Hewer A  Van Schanke A  Phillips DH  Peters LD 《Marine environmental research》2002,54(3-5):511-515

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.  相似文献   

加拿大地质勘探航磁梯度仪计划     

Hood  PJ 阎永立 《物探化探译丛》1991,(2):18-23

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金伯利岩和某些低二氧化硅高碱性岩浆的成因     

Wyll.  PJ 许德焕 《国外矿床地质》1991,(4):45-59

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海洋化石暗示出南极圈     

朱德玉 《地质科技情报》1983,(4)

在Transantarctic山脉发现的岩石中见有海洋微体化石。这标志着覆盖南极洲东部的巨大冰盾已有改变,并说明在过去的65亿年时间里,冰盾已有过几次后退。这个发现也提出了这样的可能性,即40亿年前或更早,巨大的航道穿过大陆,连接了  相似文献   

地球科学与社会     

怀利  PJ 冯明伸 《陕西地质科技情报》1995,20(4):52-54

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A Model for Structure Formation Seeded by Gravitationally Produced Matter     

Hu W  Peebles PJ 《The Astrophysical journal》2000,530(2):L61-L64

The Arecibo H i Strip Survey probed the halos of approximately 300 cataloged galaxies and the environments of approximately 14 groups with sensitivity to neutral hydrogen masses >/=107 M middle dot in circle. The survey detected no objects with properties resembling the high-velocity clouds (HVCs) associated with the Milky Way or Local Group. If the HVCs were typically MHi=107.5 M middle dot in circle objects distributed throughout groups and galaxy halos at distances of approximately 1 Mpc, the survey should have made approximately 70 HVC detections in groups and approximately 250 detections around galaxies. The null detection implies that HVCs are deployed at typical distances of 相似文献   

21世纪的原油生产与替代能源预测     

Edwa.  JD 朱德玉 《地质科学译丛》1998,15(2):19-25

为了保证随着世界人口增长和经济膨胀所需的能源供给量,必须预测原油的生产率和最终开采量,以明智地部署和采取适宜的行动。原油能够满足日益增长的需求,直到达到世界最高产量。由于常规油产量的下降而造成的能源不足将通过扩大大煤、天然气、非常规油（从焦油砂、重油与油磁岩中提炼）、核动力与水力发电、可再生能源（太阳、风和地热）的生产来填补。根据目前的估计,预测石油产量呈下降趋势,美国常规原油的最终开采量为３２９  相似文献   

A closed form slug test theory for high permeability aquifers   总被引：2，自引：0，他引：2  

Ostendorf DW  DeGroot DJ  Dunaj PJ  Jakubowski J 《Ground water》2005,43(1):87-101

We incorporate a linear estimate of casing friction into the analytical slug test theory of Springer and Gelhar (1991) for high permeability aquifers. The modified theory elucidates the influence of inertia and casing friction on consistent, closed form equations for the free surface, pressure, and velocity fluctuations for overdamped and underdamped conditions. A consistent, but small, correction for kinetic energy is included as well. A characteristic velocity linearizes the turbulent casing shear stress so that an analytical solution for attenuated, phase shifted pressure fluctuations fits a single parameter (damping frequency) to transducer data from any depth in the casing. Underdamped slug tests of 0.3, 0.6, and 1 m amplitudes at five transducer depths in a 5.1 cm diameter PVC well 21 m deep in the Plymouth-Carver Aquifer yield a consistent hydraulic conductivity of 1.5 x 10(-3) m/s. The Springer and Gelhar (1991) model underestimates the hydraulic conductivity for these tests by as much as 25% by improperly ascribing smooth turbulent casing friction to the aquifer. The match point normalization of Butler (1998) agrees with our fitted hydraulic conductivity, however, when friction is included in the damping frequency. Zurbuchen et al. (2002) use a numerical model to establish a similar sensitivity of hydraulic conductivity to nonlinear casing friction.  相似文献   

Diagnostic monitoring of a changing environment: an alternative UK perspective     

Hardman-Mountford NJ  Allen JI  Frost MT  Hawkins SJ  Kendall MA  Mieszkowska N  Richardson KA  Somerfield PJ 《Marine pollution bulletin》2005,50(12):1463-1471

Adaptive management of the marine environment requires an understanding of the complex interactions within it. Establishing levels of natural variability within and between marine ecosystems is a necessary prerequisite to this process and requires a monitoring programme which takes account of the issues of time, space and scale. In this paper, we argue that an ecosystem approach to managing the marine environment should take direct account of climate change indicators at a regional level if it is to cope with the unprecedented change expected as a result of human impacts on the earth climate system. We discuss the purpose of environmental monitoring and the importance of maintaining long-term time series. Recommendations are made on the use of these data in conjunction with modern extrapolation and integration tools (e.g. ecosystem models, remote sensing) to provide a diagnostic approach to the management of marine ecosystems, based on adaptive indicators and dynamic baselines.  相似文献