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Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   
3.
Research was conducted to assess the impact of oiling on fresh-marsh plant communities and to test the efficacy of techniques that may be used to enhance the bioremediation of crude oil spills in these environments while minimizing secondary anthropogenic impacts. To emulate field conditions, a mesocosm facility was used that houses 120 mesocosm vessels, each of 200-1 capacity. A five-way factorial treatment arrangement was used that included two substrates (inorganic, organic), two nutrient regimes (fertilized, not fertilized), two aeration levels (substrate aeration, no aeration), three oiling concentrations (0-, 5-, 10-1 m(-2) of South Louisiana Sweet Crude oil), and four vascular plant species (Alternanthera philoxeroides, Panicum hemitomon, Phragmites australis, Sagittaria lancifolia, and an unplanted control). Under the 5- and 10-1 m(-2) oiling concentrations, S. lancifolia displayed a short-term response of increased productivity, whereas P. hemitomon had the highest biomass production and photosynthetic rates at the end of the 18-month experiment. Overall plant growth and productivity, as well as oil degradation, were significantly higher in the inorganic substrate, indicating that biodegradation of oil spills in organic substrates may require a longer time period. Time-released fertilizer also stimulated plant productivity and resulted in higher soil respiratory quotients, suggestive of greater microbial activity, particularly in aerated mesocosms. The amount of oil remaining after 18 months was lowest in aerated and fertilized mesocosms containing either P. hemitomon or S. lancifolia and a substrate of low organic matter content.  相似文献   
4.
秦岭造山带历经新元古代陆块汇聚与裂解、古生代沿商丹带俯冲增生与碰撞,以及中生代沿勉略带南北两大陆块最终碰撞造山,成为一多期次构造演化而成的复合型大陆造山带.其中,最为显著的是中生代早期伴随最终碰撞造山秦岭发生强烈构造岩浆事件,在陕西商州市以西的东、西秦岭地区乃至扬子地块西北缘形成了巨量的花岗岩体,构成秦岭巨大花岗岩带.  相似文献   
5.
Perennially ice-covered lakes are found in the McMurdo Dry Valleys of southern Victoria Land, Antarctica. In contrast to temperate lakes that have diurnal photic periods, antarctic (and arctic) lakes have a yearly photic period. An unusual feature of the antarctic lakes is the occurrence of O2 at supersaturated levels in certain portions of the water column. Here we report the first sediment O2 profiles obtained using a microelectrode from a perennially ice-covered antarctic lake. Sediment cores collected in January and October 1987 from Lake Hoare in Taylor Valley show oxygenation down to 15, and in some cases, 25 cm. The oxygenation of sediments several centimeters below the sediment-water interface is atypical for lake sediments and may be characteristic of perennially ice-covered lakes. There is a significant difference between the observed January and October sediment O2 profiles. Several explanations may account for the difference, including seasonality. A time-dependent model is presented which tests the feasibility of a seasonal cycle resulting from the long photoperiod and benthic primary production in sediments overlain by a highly oxygenated water column.  相似文献   
6.
"This paper establishes and discusses briefly regional patterns and trends in crude birth, death, and natural increase rates in Russia and the USSR from the turn of the century to 1989. The study is based on a comparable regional framework and placed in the context of the demographic transition theory. It discusses sources, collection, and quality of data, as well as necessary estimating procedures. An attempt also is made to examine briefly regional age-standardized rates."  相似文献   
7.
北秦岭中生代沙河湾岩体环斑结构特征及有关问题的讨论   总被引:12,自引:0,他引:12  
该岩体的环斑结构主要发育于边部含巨斑状黑云角闪石英二长岩中 ,环斑长石粒径一般 2cm× 4cm。形态多呈自形、半自形 ,有些为卵形。环斑长石由核部钾长石和多层或单层斜长石外壳两部分构成。钾长石内核呈肉红色 ,一般是由单颗粒组成 ,具卡氏双晶 ,普遍发育规则的条纹结构 ;中心部分钾长石分子含量Or为 95 ,边部为 84。外壳斜长石牌号一般为An2 0± ,为奥长石。内核和外壳中均发育石英、斜长石、黑云母、角闪石等矿物的包裹体 ;包裹体在其边部较多 ,中部较少 ,钾长石斑晶中的石英包裹体呈不规则的凹面状和水滴状。岩石中主要矿物具有 2个世代。这些特征显示 ,沙河湾岩体中的环斑结构与典型的环斑结构是相同的。亦表明典型的环斑结构可以出现于不同的时代和构造环境。由于其形成时代和产出背景不同于典型环斑花岗岩 ,该岩体属典型环斑花岗岩还是一种新的似环斑花岗岩还有待于进一步研究。  相似文献   
8.
洪大卫  王涛  童英  王晓霞 《地学前缘》2003,10(3):231-256
近年来的研究证实 ,华北地台和大别—苏鲁造山带的中生代花岗岩与同时代的镁铁质超镁铁质岩有类似的Sr、Nd同位素特点 ,许多花岗岩和火山岩还具有类似埃达克岩的地球化学性质。在此基础上 ,根据现已积累的大量Sr、Nd同位素资料 ,从整个华北地台岩石圈的角度论证了中生代岩石圈地幔富集的性质、富集地幔发生的时代及其形成机制 ,进而探讨了岩浆活动的动力学机制 ,指出本区岩石圈富集地幔的形成是在Pangea超大陆裂解时岩石圈大规模拆沉减薄 ,被拆沉的太古宙古老地壳重循环进入地幔改变了地幔成分所致 ,说明超大陆裂解、岩石圈大规模拆沉减薄和富集地幔形成之间有密切的成因联系 ,超大陆裂解伴随着大陆地壳生长和消亡 (重循环 )的大体平衡。结合全球地震层析资料 ,进一步探讨了由俯冲大洋残片转化的下地壳同古老克拉通地壳物质在花岗岩源区中的重要意义。  相似文献   
9.
从1999年以来朝阳气售持续异常、连续4a遭受严重旱灾的实际出发,以2002年为例,分析了朝阳地区干旱的成因和特点。借以为决策服务提供科学依据。  相似文献   
10.
从 1999年以来朝阳气候持续异常、连续 4a遭受严重旱灾的实际出发 ,以 2 0 0 2年为例 ,分析了朝阳地区干旱的成因和特点 ,借以为决策服务提供科学依据  相似文献   
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