排序方式： 共有38条查询结果，搜索用时 156 毫秒
1.
该文分别选用不同浓度的海螺酶 ;葡萄糖、蔗糖、甘露醇、山梨醇、氯化钠 5种渗透剂 ;维生素 C和甘露醇 2种抗氧化剂 ;对条斑紫菜酶解单细胞的成活率进行了研究。实验结果表明 :用浓度为 10 %的海螺酶酶解条斑紫菜 ,成活率最高 ;选用 2 mol/ L葡萄糖作渗透剂 ,效果最好 ,成活率达85.2 % ;加入抗氧化剂对细胞成活率没有影响 相似文献
2.
羊栖菜茎部愈伤组织再生苗的解剖学研究 总被引：1，自引：0，他引：1
利用石蜡切片法对羊栖菜纵裂主枝上愈伤组织再生苗的发生过程进行了形态解剖学观察。发现此种成苗方式是在主枝 (茎 )或假根处由外皮层分裂形成愈伤组织 ,再由愈伤组织分化成苗的一种无性繁殖方式。愈伤组织形成再生苗有望成为羊栖菜人工养殖种苗的来源之一。 相似文献
3.
Filamentous Bangia, which are distributed extensively throughout the world, have simple and similar morphological characteristics. Scientists can classify these organisms using molecular markers in combination with morphology. We successfully sequenced the complete nuclear ribosomal DNA, approximately 13 kb in length, from a marine Bangia population. We further analyzed the small subunit ribosomal DNA gene (nrSSU) and the internal transcribed spacer (ITS) sequence regions along with nine other marine, and two freshwater Bangia samples from China. Pairwise distances of the nrSSU and 5.8S ribosomal DNA gene sequences show the marine samples grouping together with low divergences (00.003; 0–0.006, respectively) from each other, but high divergences (0.123–0.126; 0.198, respectively) from freshwater samples. An exception is the marine sample collected from Weihai, which shows high divergence from both other marine samples (0.063–0.065; 0.129, respectively) and the freshwater samples (0.097; 0.120, respectively). A maximum likelihood phylogenetic tree based on a combined SSU-ITS dataset with maximum likelihood method shows the samples divided into three clades, with the two marine sample clades containing Bangia spp. from North America, Europe, Asia, and Australia; and one freshwater clade, containing Bangia atropurpurea from North America and China. 相似文献
4.
Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa
沈颂东 《中国海洋湖沼学报》2008,26(4)
In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis. 相似文献
5.
Natural Hazards - The paper presented a smoothed particle hydrodynamics (SPH) method to study the three-dimensional (3D) tidal bore scenarios. The SPH method is a mesh-free particle modeling... 相似文献
6.
浒苔(Enteromorpha prolifera)生长及孢子释放的生态因子研究 总被引：26，自引：0，他引：26
通过考察浒苔属浒苔藻体生长及孢子释放的生态因子条件,确定其各单因子的适应范围及最适范围.结果表明:浒苔藻体生长对盐度、温度、光照强度和酸碱度的适应范围分别为 16～40、10 ℃～30 ℃、＞9 μmol/m2s、6～10,最适范围分别为 24～28、20 ℃～25 ℃、＞18 μmol/m2s、8～9,在盐度、温度、光照强度和酸碱度分别为24、25 ℃、72 μmol/m2s、8 的条件下达到最大生长量;浒苔释放孢子对盐度、温度、光照强度和酸碱度的适应范围分别为 12～40、15 ℃～35 ℃、＞9 μmol/m2s、6～10,最适范围分别为 28～40、20 ℃～35 ℃、＞36 μmol/m2s、8～9,在盐度、温度、光照强度和酸碱度分别为32、35 ℃、144 μmol/m2s、9 的条件下达到最大释放量.浒苔藻体生长及孢子释放的各单因子适应范围基本相同,最适范围区别明显. 相似文献
7.
The paper presents an Incompressible Smoothed Particle Hydrodynamics (ISPH) method to simulate wave interactions with a porous medium. The SPH method is a mesh free particle modeling approach that is capable of tracking the large deformation of free surfaces in an easy and accurate manner. The ISPH method employs a strict incompressible hydrodynamic formulation to solve the fluid pressure and the numerical solution is obtained by using a two-step semi-implicit scheme. The ISPH flow model solves the unsteady 2D Navier–Stokes (NS) equations for the flows outside the porous media and the NS type model equations for the flows inside the porous media. The presence of porous media is considered by including additional friction forces into the equations. The developed ISPH model is first validated by the solitary and regular waves damping over a porous bed and the solitary wave interacting with a submerged porous breakwater. The convergence of the method and the sensitivity of relevant model parameters are discussed. Then the model is applied to the breaking wave interacting with a breakwater covered with a layer of porous materials. The computational results demonstrate that the ISPH flow model could provide a promising simulation tool in coastal hydrodynamic applications. 相似文献
8.
１ＩＮＴＲＯＤＵＣＴＩＯＮＢａｓｅｄｏｎｔｈｅｍｏｕｎｔａｉｎｓｔｒｅａｍｃｌａｓｉｆｉｃａｔｉｏｎａｎｄｈａｚａｒｄｚｏｎｅｍａｐｐｉｎｇ（Ｗａｎｇｅｔａｌ,１９９６;ａｎｄＷａｎｇｅｔａｌ,１９９８）,ａｓｗｅｌａｓｔｈｅｉｎｖｅｓｔｉｇａｔｉｏ... 相似文献
9.
I~ODWrIONExtraction of DNA from Porphyra po~nsis is very difficult due tO its high content ofpolysaccharides. TO avoid the interference Of POlysaccharides, HOng et al. (1992) used LiCI tosoften Porphyra thalli cells to release DNA directly without lysing the thalli, hoWever, the procedure is problematic in the isolation of high-quality DNA. Kitade et al. (1996) established a complicated procedure which includes the grindiflg of material in liquid nitrogen, lysing of material inextra… 相似文献
10.
条斑紫菜单细胞固体培养的初步研究 总被引：1，自引：0，他引：1
成熟条斑紫菜叶状体酶解后获得单细胞,分别培养于添加了不同浓度N,P营养盐的0．5％和10％的琼胶培养基上,观察细胞的分化发育情况。结果显示：(1)营养细胞大部分先发育为细胞团,细胞团放散孢子,再由孢子萌发形成小苗。其余营养细胞极性分裂形成正常细胞苗或带假根的形状不规则的片状体。孢子茁和营养细胞直接形成的幼苗生长到一定大小均可放散单孢子,单孢予能够正常萌发形成单孢子苗。(2)在0．5％的琼胶培养基上或较高浓度的营养盐中,细胞发育快。添加5mg／L NO3-N和0．5mg／L H2PO4—P营养盐不能满足细胞生长发育的需要,培育20d绝大部分仍为单细胞,不能形成正常幼苗。 相似文献