Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels   总被引：5，自引：0，他引：5  

Scholz S  Kordes C  Hamann J  Gutzeit HO 《Marine environmental research》2004,57(3):235-244

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.  相似文献   

Metal levels in seston and marine fish flesh near industrial and metropolitan centres in South Australia.     

J W Edwards  K S Edyvane  V A Boxall  M Hamann  K L Soole 《Marine pollution bulletin》2001,42(5):389-396

Port Pirie is the site of the largest lead smelter in the world, depositing 250 t of zinc, and 100 t of lead annually into Spencer Gulf. Barker Inlet is adjacent to metropolitan Adelaide, and receives unknown quantities of urban and industrial discharges. Both areas are sites of major commercial and recreational fisheries, contained within delicately balanced marine wetland ecosystems, comprising large areas of mangrove and seagrass habitats. Aldrichetta forsteri and Sillago schomburgkii are major species within these fisheries and as estuarine-dependent species were chosen for this study as indicator species for the detection and monitoring of pollutant impacts in the nearshore marine ecosystems of South Australia. Seston sediment collectors were deployed at each site and analysed seasonally for the presence of cadmium, lead and copper. Flesh samples from A. forsteri and S. schomburgkii were examined seasonally for the presence of cadmium, lead and copper and the results correlated with levels found in the seston sediment at each site. Metal concentrations were also correlated with a biomarker of genotoxicity measured in the same animals (micronuclei in erythrocytes) that were reported previously. Seston levels of cadmium, lead and copper were highest at Port Pirie, followed by Barker Inlet and were lowest at Wills Creek, with cadmium undetectable at the latter site. Metals in seston varied considerably with season, with generally higher levels in winter samples. In fish flesh, metal levels followed broadly similar trends as for seston. Spearman rank correlations between metals in seston and in flesh were strongly positive. There was also a significant correlation between flesh concentrations of each metal and the frequency of micronuclei in erythrocytes. This study has shown that seston concentration of pollutant metals are high in areas of industrial activity, and that these levels are also reflected in metal content of fish flesh. Mean flesh levels of cadmium and copper did not exceed Australian health based maximum permitted levels of fish for human consumption, whereas flesh levels of lead in fish from Port Pirie and Barker Inlet exceeded these standards in each of the seasons monitored. This may represent a significant dietary source of lead in humans, especially at Port Pirie where human lead exposure from terrestrial sources is important. There may also be the potential for accumulation of metals in residents of metropolitan Adelaide whose diets are high in fish (and/or crustaceans), particularly estuarine-dependent species, such as A. forsteri and S. schomburgkii. The study also showed that a non-specific biomarker of genotoxicity (micronuclei in erythrocytes) is potentially useful as a monitoring technique in fish species to evaluate their exposure and genotoxic responses to pollutants in South Australian waters. These data represent a snapshot of the current situation in this area and may act as background levels against which future improvements or decrements in water quality may be compared.  相似文献   

The dynamics of phytoplankton, bacteria and heterotrophic flagellates at two Banks near Magdeburg in the River Elbe (Germany)     

Bernhard Karrasch  Maren Mehrens  Yvonne Rosenlcher  Karla Peters 《Limnologica》2001,31(2)

The main objectives of this study were to describe the seasonal standing stock dynamics of phytoplankton, bacterioplankton and heterotrophic flagellates in the highly eutrophic River Elbe (Germany), and to compare the seasonal patterns observed with other streams. Emphasis was placed on examining and assessing abiotic and biotic controlling factors influencing the structure and dynamics of the riverine plankton. All the physico-chemical and biological parameters determined were within the range or somewhat higher (in the case of phytoplankton abundance and biomass) than reported for other large streams. The underwater light conditions resulting from atypically short phytoplankton growth periods of about 6 months per year and the low phytoplankton carbon to chl a ratio of 23 were identified as a major limiting factor for phytoplankton development in the River Elbe. The seasonal distribution pattern of bacterioplankton indicated probable tight trophodynamical coupling both with phytoplankton and with heterotrophic flagellates, whereas heterotrophic flagellates showed a more trophic link with bacterial densities. Although approximately constant DOC and DON levels throughout the year sustained bacterial growth rates, during the phytoplankton growing season an increase of bacterial standing stocks was observed. Although the left-bank sampling site of the Elbe is strongly influenced by the tributaries Mulde and Saale containing higher concentrations of chloride, nitrogen nutrients, heavy metals and organic pollutants, no clear differences were observed between the two sides of the river concerning the biological parameters measured. Possible reasons and the slightly higher phytoplankton abundance and diversity at the right bank are discussed.  相似文献   

International Association of Geomorphologists Newsletter No. 18 (1/2001)     

C. Embleton‐Hamann 《地球表面变化过程与地形》2001,26(7):803-806

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Sediment rejection by recent scleractinian corals: a key to palaeo-environmental reconstruction     

Julia A. E. B. Hubbard  Yvonne P. Pocock 《International Journal of Earth Sciences》1972,61(2):598-626

26 of the 59 living species of scleractinian corals found in Western Atlantic and Caribbean waters, less than 68 metres deep, and two fungiid genera from the Pacific are analysed in terms of their behaviour, sediment rejecting properties, functional morphology and distribution patterns.The contrasting behaviour and skeletal structure of the two solitary fungiids provides evidence for the construction of a theoretical mechanical model which is applied to the subsequent interpretation of the more complex colonial reef corals from the Atlantic province.Regional distribution patterns, growth forms and calical orientations of the Atlantic species and their associated sediment types were noted in dives across transects in extreme environments in the Caribbean. Representative samples were then selected from the five functional ecological niches in the Florida keys, and subjected to inundations of sized and painted carbonate sand particles in the laboratory. The coral was filmed before the sedimentary influx, on impact and during rejection. The skeletons were then extracted and dissected to construct a mechanical model compatible with their behavioural functions.There are four means of sediment rejection: distension by the stomodeal up-take of water, tentacular action, ciliary beat, and mucus entanglement. Differences in sediment rejection are ascribed to two factors (1) variations in the polyps' distensional capacity, and (2) the geometry of the calice.A theoretical model of characteristic scleractinian distribution patterns on an Atlantic patch reef mirrors regional distribution patterns. The sediment rejecting experiments are used as additional evidence for interpreting other behavioural activities of greater consequence e.g. food gathering and the removal of excrement.Theoretical models are described for the relationship of calical form to polypal function and calcical orientation to distribution patterns on the corallum; and also variations in growth form. Ecological distributions, in local patch reef and regional occurrences, are outlined in terms of the recent scleractinian corals of the Atlantic province. These models are tested against some rugose and tabulate coral distributions in the Irish Carboniferous.

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Zusammenfassung 26 der 59 Korallenarten (Scleractinia), die im westlichen Atlantik und der Karibischen See in weniger als 68 m Wassertiefe leben, und 2 Fungia-Gattungen des Pazifiks werden auf Verhalten, Fähigkeit Sediment abzuschütteln und funktioneller Morphologie untersucht. Aufgrund der Gegensätze in Verhalten und Skelettstruktur der beiden Fungia-Einzelkorallen wird ein theoretisches Modell erarbeitet, das dann auf die komplizierteren koloniebildenden Korallen angewandt wird.Regionale Verteilung, Wuchsformen und Kelchorientierung der atlantischen Korallen und die dazugehörigen Sedimente wurden in Tauchgängen in extremen Bereichen der Karibischen See erfaßt. Repräsentative Korallen-Exemplare wurden dann von fünf ökologisch definierten Bereichen der Florida Keys gesammelt und im Labor mit sortierten und gefärbten Karbonatsandkörnern überschüttet. Die Korallen wurden vor dem Experiment, während des Schüttens und des Abschüttelns gefilmt. Danach wurden die Skelette herauspräpariert und ein mechanisches Modell entwickelt, das die verhaltensbedingten Funktionen berücksichtigt.Das Sediment wird mit Hilfe von vier Mechanismen abgeschütteln: Ausdehnung der Weichteile durch Aufnahme von Wasser, Tentakelbewegungen, Ziliarbewegungen und Schleimumwicklung. Unterschiede in der Fähigkeit, Sediment abzuschütteln, wurden auf 1. Unterschiede in der Fähigkeit des Polypen, sich auszudehnen, und 2. Unterschiede in der Kelchform zurückgeführt.Ein theoretisches Modell der Korallenverteilung auf einem isolierten Riff spiegelt die Grundzüge regionaler Verteilung wider. Die Sedimentabschüttel-Experimente ergeben zusätzliche Anhaltspunkte für die Interpretation anderer Verrichtungen von größerer Bedeutung, z. B. Nahrungsaufnahme und Entfernung von Exkrementen. Theoretische Modelle für das Verhältnis von Kelchform zu Polypenfunktion und von Kelchorientierung zur Position auf dem Korallenstock und zu Wuchformunterschieden werden beschrieben.Ökologische Verteilungen, lokal und regional, werden anhand der rezenten Korallen des Atlantiks aufgezeigt. Diese Modelle werden dann am Beispiel der Verteilung rugosen und tabulaten Korallen im Karbon Irlands getestet.

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Résumé 26 espèces vivantes de coraux scléractiniens sur 59, furent découvertes dans l'Atlantique-Ouest et les eaux des Caraïbes, à moins de 68 m de fond; 2 genera fungiid du Pacifique sont analysés en fonction de leur comportement, des propriétés de rejet des sédiments dont ils font preuve, de leur morphologie fonctionelle et de leurs reséaux de distribution.Le contraste dans le compartement et dans la structure squelettique des deux fungiids solitaires, justifie la construction d'un modèle théorique mécanique qui est appliqué à l'interprétation suivante des coraux de récif coloniaux et plus complexes que l'on trouve dans la région atlantique.Les réseaux de distribution régionale, les formes de croissances d'orientations calicales de l'espèce atlantique et les types de sédiments qui leur sont associés, ont été remarqués lors de plongées aux environs extrêmes de la mer Caraïbe. Des échantillons représentatifs ont ensuite été sélectionnés dans les cinq niches écologiques fonctionelles des cayes de Floride. Puis ont été soumis, en laboratoire à des inondations de particules de sable carbonaté taillées et teintes. Le corail a été filmé avant l'entrée des sédiments, pendant l'opération et pendant le rejet. Les squelettes, ont ensuite été extraits et disséqués pour construire un modèle mécanique compatible avec leurs fonctions de compartement.Il existe quatre modes de rejet des sédiments: dilatation par absorbsion stomodéal d'eau, action tentaculaire, action ciliaire et enveloppement de mucus.Les différences dans le rejet de sédiments sont imputables a deux facteurs (1) les variations de la capacité dimensionelle du polype et (2) la géométrie du calice. Un modèle théorique des réseaux le distribution scléractiniènne, charactéristique sur un petit récif atlantique, réflecte des sébeaux de distribution régionale. Les expériences sur le rejet de sédiments, sont utilisées comme preuve supplémentaire à l'interprétation d'autres activités de comportement, comme par exemple, la recherche de nourriture ou l'émission d'éxcréments.Les modèles théoriques sont décrits pour permettre le rapport entre la forme calicale et la fonction polypale, plus entre l'orientation calicale et les résaux de distribution sur le corallum, ainsi que pour étudier les variations dans les formes de croissance. L'étude des coraux scléractiniens récents de la province atlantique, permet d'établir une ébauche de leur répartition écologique, aussi bien sur les petits récifs locaux qu'au niveau des incidences régionales. Les modèles sont également testés grace à des répartitions de coraux rugueux et tabulates dans le Carbonifière Irlandais.

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Structural characteristics of the halite fabric type ‘Kristallbrocken’ from the Zechstein Basin with regard to its development     

Yvonne Küster  Bernd Leiss  Michael Schramm 《International Journal of Earth Sciences》2010,99(3):505-526

The Kristallbrocken are a characteristic centimetre- to decimetre-sized, laminated halite fabric type occurring in the Stassfurt Formation in the Zechstein Basin. Up to now, the nature of the Kristallbrocken, i.e. if they are relics of fine-grained, polycrystalline halite beds or clasts of ‘single crystal-layers’, as well as the deformation mechanisms of this halite type, were not clear from the literature. Drill core material from the salt deposit Teutschenthal at the southern rim of the Zechstein Basin now allowed investigating less intensely deformed samples for the first time. The deformational behaviour of these Kristallbrocken ranges from brittle to ductile, which is evidenced by fractured Kristallbrocken on the one hand and weakly bent or even folded Kristallbrocken on the other hand. Local X-ray texture measurements demonstrated that the Kristallbrocken are definitely single crystals and that they can be regarded as relics of formerly larger ‘single crystal-layers’ of up to several dm² in size. The folded Kristallbrocken clearly display by their single grain texture characteristics that their crystal lattice is bent, which was most likely enabled by a kind of flexural-shear folding, and did not develop after deformation from a fine-grained aggregate by recrystallisation. Due to their monocrystallinity, their originally large size, and the solid inclusions forming the internal lamination, the Kristallbrocken have clearly stronger rheological properties than the surrounding fine- to coarse-grained polycrystalline rock salt, and thus also deform by fracturing.  相似文献   

Patterns of Irrigated Agricultural Land Conversion in a Western U.S. Watershed: Implications for Landscape-Level Water Management and Land-Use Planning     

J. Mark Baker  Yvonne Everett  Lora Liegel  Rob Van Kirk 《社会与自然资源》2014,27(11):1145-1160

In irrigated agricultural landscapes, land-use conversion may have landscape-level social, hydrological, and ecological effects. We used geographic information systems (GIS) and interviews to analyze development effects on irrigation in the Henry's Fork Watershed, Idaho. Farmers developed irrigation there in the 19th century, and incidental recharge from canal seepage and flood irrigation raised groundwater levels and expanded wetlands. Cohesive culture in agricultural communities sustained irrigation systems until amenity-driven demographic shifts beginning in the 1970s led to development approved by local governments with land-use planning but no water-management authority. Although only 5% of irrigated land has been converted, development has fragmented irrigated landscapes and made canal-system operation more difficult, potentially reducing groundwater levels, wetland extent, and return flows critical to downstream irrigators and fish and wildlife. We discuss future scenarios, highlighting the need for increased communication among local and state governments regarding land use and water management in irrigated landscapes across the West.  相似文献   

International Association of Geomorphologists Newsletter No. 17 (3/2000)     

C. Embleton‐Hamann 《地球表面变化过程与地形》2000,25(13):1479-1483

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The fusion crust of the Winchcombe meteorite: A preserved record of atmospheric entry processes     

Matthew J. Genge  Luke Alesbrook  Natasha V. Almeida  Helena C. Bates  Phil A. Bland  Mark R. Boyd  Mark J. Burchell  Gareth S. Collins  Luke T. Cornwell  Luke Daly  Hadrien A. R. Devillepoix  Matthias van Ginneken  Ansgar Greshake  Daniel Hallatt  Christopher Hamann  Lutz Hecht  Laura E. Jenkins  Diane Johnson  Rosie Jones  Ashley J. King  Haithem Mansour  Sarah McMullan  Jennifer T. Mitchell  Gavyn Rollinson  Sara S. Russell  Christian Schröder  Natasha R. Stephen  Martin D. Suttle  Jon D. Tandy  Patrick Trimby  Eleanor K. Sansom  Vassilia Spathis  Francesca M. Willcocks  Penelope J. Wozniakiewicz 《Meteoritics & planetary science》2024,59(5):948-972

Fusion crusts form during the atmospheric entry heating of meteorites and preserve a record of the conditions that occurred during deceleration in the atmosphere. The fusion crust of the Winchcombe meteorite closely resembles that of other stony meteorites, and in particular CM2 chondrites, since it is dominated by olivine phenocrysts set in a glassy mesostasis with magnetite, and is highly vesicular. Dehydration cracks are unusually abundant in Winchcombe. Failure of this weak layer is an additional ablation mechanism to produce large numbers of particles during deceleration, consistent with the observation of pulses of plasma in videos of the Winchcombe fireball. Calving events might provide an observable phenomenon related to meteorites that are particularly susceptible to dehydration. Oscillatory zoning is observed within olivine phenocrysts in the fusion crust, in contrast to other meteorites, perhaps owing to temperature fluctuations resulting from calving events. Magnetite monolayers are found in the crust, and have also not been previously reported, and form discontinuous strata. These features grade into magnetite rims formed on the external surface of the crust and suggest the trapping of surface magnetite by collapse of melt. Magnetite monolayers may be a feature of meteorites that undergo significant degassing. Silicate warts with dendritic textures were observed and are suggested to be droplets ablated from another stone in the shower. They, therefore, represent the first evidence for intershower transfer of ablation materials and are consistent with the other evidence in the Winchcombe meteorite for unusually intense gas loss and ablation, despite its low entry velocity.  相似文献   

Tide-induced variability in beach surface moisture: Observations and modelling     

Laura B. Brakenhoff  Yvonne Smit  Jasper J. A. Donker  Gerben Ruessink 《地球表面变化过程与地形》2019,44(1):317-330

The moisture content w_s of a beach surface strongly controls the availability of sand for aeolian transport. Our predictive capability of the spatiotemporal variability in w_s, which depends to a large extent on water table depth, is, however, limited. Here we show that water table fluctuations and surface moisture content observed during a 10-day period on a medium-grained (365μm) planar (1:30) beach can be predicted well with the nonlinear Boussinesq equation extended to include run-up infiltration and a soil–water retention curve under the assumption of hydrostatic equilibrium. On the intertidal part of the beach the water table is observed and predicted to continuously fall from the moment the beach surface emerges from the falling tide to just before it is submerged by the incoming tide. We find that on the lower 30% of the intertidal beach the water table remains within 0.1–0.2 m from the surface and that the sand is always saturated (w_s≈20%, by mass). Higher up on the intertidal beach, the surface can dry to about 5% when the water table has fallen to 0.4–0.5 m beneath the surface. Above the high-tide level the water table is always too deep (>0.5 m) to affect surface moisture and, without precipitation, the sand is dry (w_s < 5 − 8%). Because the water table depth on the emerged part of the intertidal beach increases with time irrespective of whether the (ocean) tide falls or rises, we find no need to include hysteresis (wetting and drying) effects in the surface-moisture modelling. Model simulations suggest that at the present planar beach only the part well above mean sea level can dry sufficiently (w_s < 10%) for sand to become available for aeolian transport. ©2018 The Authors. Earth Surface Processes and Landforms published by John Wiley & Sons Ltd.  相似文献