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The present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (Gr), and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol (PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could significantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod. 相似文献
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研究了不同浓度的抗冻剂二甲基亚砜（DMSO）和不同保存时间对真鲷（Pagrus major）冻精质量的影响。试验结果表明,DMSO体积分数和保存时间对冻精的受精率、孵化率影响显著。15％二甲基亚砜作为抗冻剂保存10～60d的冻精受精率、孵化率与鲜精无差异,而保存360d的冻精受精率、孵化率开始出现下降。12％,18％,21％DMSO保存60d的冻精受精率差异不显著,均高于90％,而对于保存360d的冻精受精率、孵化率均显著地降低。 相似文献
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