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东海原甲藻的分子鉴定 总被引：4，自引：2，他引：4
采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准. 相似文献
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SO2 is very rapidly hydrated to sulfurous acid in water solution at pH value above 6.0,whereby sulfite is yielded from the disassociation of protons.We aimed to improve the sulfite transformation efficiency and provide a basis for the direct utilization of SO2 from flue gas by a microalgal suspension.Chlorella sp.XQ-20044 was cultured in a medium with 20 mmol/L sodium sulfite under different physicochemical conditions.Under light conditions,sulfite concentration in the algal suspension reduced linearly over time,and was completely converted into sulfate within 8 h.The highest sulfite transformation rate(3.25 mmol/(L·h)) was obtained under the following conditions:35°C,light intensity of 300μmol/(m2·s),NaHCO3 concentration of 6 g/L,initial cell density(OD540) of 0.8 and pH of 9-10.There was a positive correlation between sulfite transformation rate and the growth of Chlorella,with the conditions favorable to algal growth giving better sulfite transformation.Although oxygen in the air plays a role in the transformation of SO2-3 to SO2-4,the transformation is mainly dependent on the metabolic activity of algal cells.Chlorella sp.XQ-20044 is capable of tolerating high sulfite concentration,and can utilize sulfite as the sole sulfur source for maintaining healthy growth.We found that sulfite 20 mmol/L had no obvious effect on the total lipid content and fatty acid profiles of the algae.Thus,the results suggest it is feasible to use flue gas for the mass production of feedstock for biodiesel using Chlorella sp.XQ-20044,without preliminary removal of SO2,assuming there is adequate control of the pH. 相似文献
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