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长囊水云(Ectocarpus siliculosus)是一种重要的海洋褐藻,是褐藻研究的模式生物.以其叶绿体全基因组序列为基础,对密码子使用特征进行分析.结果显示,叶绿体基因组的GC尤其是GC3值较低,分别为31.8％和17.32％,偏向使用以A或T碱基结尾的密码子;全基因组具有较小的有效密码子数(ENC)值和较高的密码子适应指数(CAI)值,表明其具有较高的同义密码子使用偏性;密码子使用特征受到基因表达水平上的自然选择以及基因碱基组成中性突变的双重影响,其中选择的作用对高表达基因密码子使用偏性的影响比较突出,而突变是低表达基因密码子使用偏性的主要影响因素.另外,首次确定UUU,GUU,UCA等12个密码子为长囊水云叶绿体基因组的最优密码子. 相似文献
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Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments,and consequently may have highly effi cient ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)activity for carbon assimilation.In our study,we cloned the full-length Rubisco gene from S.japonica(SJ-rbc).It contained an open reading frame for a large subunit gene(SJ-rbcL)of 1 467 bp,a small subunit gene(SJ-rbcS)of 420 bp,and a SJ-rbcL /S intergenic spacer of 269 bp.The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa,5.81 and 15.84 kDa,4.71,respectively.After induction with 1 mmol/L isopropyl-β-Dthiogalactopyranoside for 5 h and purifi cation by Ni 2+ affi nity chromatography,electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein.Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night.This observation implied that Rubisco played a role in normal gametophytic growth and development.In juvenile sporophytes,mRNA levels of SJ-rbcL,carbonic anhydrase,Calvin-BensonBassham cycle-related enzyme,and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance.Similarly,expression of these genes was up-regulated under blue light irradiance at 350 μmol/(m 2·s).Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements. 相似文献
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