Influence of Ultrasonic Treatment on the Allergenic Properties of Shrimp （Penaeus vannamei ） Allergen     

LI Zhenxing LIN Hong CAO Limin 《中国海洋大学学报(英文版)》2006,5(2):115-118

The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0 ℃ or 50 ℃ for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA （Ci-ELISA） to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0℃. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50 ℃. In the determination of allergenicity with CiELISA, a reduction in IgE binding was also observed.  相似文献   

都思兔河中段河水化学成分的形成机制分析     

钱会  宋秀玲  窦妍  候广才  赵振宏 《水文地质工程地质》2008,35(6):103-106

在分析研究区地质及水文地质条件的基础上,根据对各类水样资料的研究,发现在都思兔河中段,对于相对惰性的组分,河水的化学成分由湖水与地下水混合形成;但对于易发生化学反应的组分,湖水在向下游运动的过程中,其化学成分明显受到了化学反应的影响。论文使用Cl-质量平衡方法,计算出该段河水中地下水的补给比例在50%以上;并且根据研究区的具体条件及矿物饱和指数的计算结果,确定石膏的溶解、方解石和白云石的沉淀以及CO2逸出是河水化学成分形成的主要化学作用,通过对化学反应量的计算,恢复了湖水与地下水仅发生了简单混合作用的混合水的成分。  相似文献   

Numerical simulation of three-dimensional tidal current in the Bobal Sea     

Den Zhenxing  Yang Lianwu  Jose Ozer 《海洋学报(英文版)》1994,13(2):155-172

Ｎｕｍｅｒｉｃａｌｓｉｍｕｌａｔｉｏｎｏｆｔｈｒｅｅ－ｄｉｍｅｎｓｉｏｎａｌｔｉｄａｌｃｕｒｒｅｎｔｉｎｔｈｅＢｏｂａｌＳｅａ￥ＤｅｎＺｈｅｎｘｉｎｇ;ＹａｎｇＬｉａｎｗｕ;ａｎｄＪｏｓｅＯｚｅｒ（ＲｅｃｅｉｖｅｄＭａｙ１,１９９３;ａｃｃｅｐｔｅｄ...  相似文献   

3种鲍16S rRNA基因片段序列的初步研究   总被引：13，自引：0，他引：13  

杨建敏  郑小东  王如才  宋志乐  孙振兴 《中国海洋大学学报(自然科学版)》2003,33(1):36-40

本文对引自日本的盘鲍 (H aliotisdiscusdiscus)、皱纹盘鲍 (H.discushannai)和大鲍 (H.gi-gantiea) 3个自然群体的线粒体 DNA 1 6S r RNA基因片段进行了扩增和测序 ,分析了 52 8bp的碱基序列 ,结果显示 :3种鲍的基因序列中 A+T含量为 56.74%～ 57.1 2 %。 3个自然群体间碱基片段序列差异不显著 ,盘鲍与皱纹盘鲍、大鲍彼此均仅有 1处核苷酸检测到变异 ,皆为碱基转换 ,同源性为99.81 % ;皱纹盘鲍与大鲍有 2处碱基转换 ,同源性为 99.61 %。 1 6Sr RNA基因在鲍属内表现出很高的保守性。  相似文献   

Feeding habit and seasonal variation of stomach contents of sand dabs, Pseudopleuronectes yokohamae, in the South Bohai Sea     

Dou Shuozeng  Yang Jiming 《海洋学报(英文版)》1993,12(4):629-635

Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.  相似文献   

鱼类摄食生态研究的理论及方法   总被引：21，自引：1，他引：21  

窦硕增 《海洋与湖沼》1996,27(5):556-561

综合分析了鱼类摄食生态研究的理论，方法及其应用中的诸多问题：胃含物分析法，营养生境及食物重叠，食物链与食物网，营养结构与营养级，摄食器官的形态特征与摄食行为的关系，食物选择以及生理生太因子对鱼类摄食的影响；提出了食物排序的概念、优势度指数Ｄ及其判定公式，并以此比较客观地建立了摄食生态相似的鱼类群落主次食物的划分标准。  相似文献   

东海大陆架异养细菌的生态分布   总被引：4，自引：0，他引：4  

陈騳  钱振儒  王之珉  沈世泽  杨肇惠 《海洋科学集刊》1982,(19):1-10

从海洋物质循环的观点来看,异养细菌在分解有机物质和无机化过程中起着极重要的作用。从海洋食物链的角度来看,分解各种类型有机物而得以增殖的异养细菌自身也是海洋原生动物、浮游动物及底栖动物的营养源。异养细菌在海洋生态系中占有极重要的位置。对海洋中某些细菌的代谢活动与其他海洋生物之间的相互关系的研究日益受到普遍的重视。 海洋异养细菌的生态分布资料已有不少报道。但至今尚未有人发表过东海大陆架海域海洋微生物生态调查的报告。东海大陆架海域江河交汇人海,大陆对海洋的影响极为突出,水文和底质情况复杂,水产资源丰富,因而特别富有多样化的有机物来源。调查和了解这一海域中异养细薗生态分布规律,并研究其参与物质转化过程的特异性,不仅有助于深入研究海洋生态学问题,而且对于阐述东海大陆架的特点也极有价值。本文着重讨论异养细菌在东海大陆架的生态分布特征及菌群组成的特点。  相似文献   

Influence of large-diameter pipe pile driving on surrounding marine soils and adjacent submarine pipelines     

Jinzhong Dou  Zhong-Jie Zhang 《Marine Georesources & Geotechnology》2021,39(1):15-33

Abstract

With the large-scale development and utilization of ocean resources and space, it is inevitable to encounter existing submarine facilities in pile driving areas, which necessitates a safety assessment. In this article, by referring to a wharf renovation project as a reference, the surrounding soil response and buried pipe deformation during pile driving in a near-shore submarine environment are investigated by three-dimensional (3D) numerical models that consider the pore water effect. Numerical studies are carried out in two different series: one is a case of a single pile focusing on the effect of the minimum plane distance of the pile–pipe, and the other is a case of double piles focusing on the effect of the pile spacing.  相似文献   

皱纹盘鲍三倍体生长的初步研究   总被引：13，自引：0，他引：13  

孙振兴  宋志乐 《海洋湖沼通报》1992,(4):70-75

将用低温休克法和细胞松弛素B诱导法,获得的皱纹盘鲍(Haliotis discus hannai Ino)三倍体诱导群与二倍体的个体,在室内同样条件下饲育,对其生长情况进行了比较,结果表明:(1)三倍体与二倍体的当年稚鲍在壳长和体重的增长上无明显差异。(2)三倍体与二倍体的2龄鲍,在第二年前期生长无明显差异,但从第二年后期开始,三倍体的壳长、体重增长逐渐优于二倍体。(3)统计学分析表明,三倍体与二倍体的3龄鲍在体重、软体部和足肌的增长方面,存在着明显差异。(4)在室内饲育19个月的鲍,三倍体诱导群的壳长比二倍体大10.2％,体重比二倍体大20.1％,足肌湿重比二倍体大17.6％。  相似文献   

Relationship between hydrocarbons and bacterial activity in mediterranean sediments: Part 2—hydrocarbon degrading activity of bacteria from sediments     

E. Azoulay  M. Colin  J. Dubreuil  H. Dou  G. Mille  G. Giusti 《Marine environmental research》1983,9(1):19-36

In marine sediments from the Mediterranean coast polluted by oil spills (Etang de Berre) high bacterial activity is responsible for self-purification, samples collected from these areas showing a bacterial density several orders of magnitude higher than samples from non-polluted areas (Isle des Embiez). Between 60 and 80 % of the heterotrophic bacteria in the polluted areas are hydrocarbon-degrading, compared with 0.01 % in the non-polluted area.Several strains of hydrocarbon utilisers were isolated, a mixture of strains from each biotope was taken to represent a ‘biotope population’ and their activity towards different types of hydrocarbons (n-alkanes, iso-alkanes, cycloalkanes, aromatics) was determined.With ammonia as the nitrogen source the ‘biotope population’ from the polluted area degraded hydrocarbons much more readily than the ‘biotope population’ from the non-polluted area. With nitrate as the nitrogen source the degradation was much reduced, and, for some hydrocarbons, ceased in both ‘populations’.Individual hydrocarbons encouraged the growth of individual strains in the ‘biotope population’. In natural mixtures of hydrocarbons all strains grew well and degraded aliphatics. There was a good correlation between the respiratory activity of the ‘biotope population’ and the ability of the ‘population’ to utilise hydrocarbons for growth.  相似文献