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The aim of this study was to investigate the potential application of the beta-d-glucuronidase (GLUase) activity measurement for the routine detection and quantification of E. coli in marine bathing waters. GLUase activity was measured as the rate of hydrolysis of 4-methylumbelliferyl-beta-d-glucuronide. Culturable E. coli were quantified by the most probable number (MPN) microplate method. Both methods were applied to a large set of seawater samples. Significant correlation was found between the log of GLUase activity and the log of culturable E. coli. The mean coefficient of variation (CV) of the GLUase activity was less than 15% at concentrations around the current standards of International regulations whereas the CV of the microplate method was around 30%. When samples were stored at 4 degrees C and 20 degrees C, the mean CV of the GLUase activity remained below 15% up to 6 hours after sample collection whereas the range of variation of the microplate method varied between 10 and 50%. We concluded that the GLUase activity is an operational, reproducible, simple, very rapid and low cost method for the real-time enumeration of E. coli in bathing waters and should be preferred to the microplate method. The GLUase activity method should be routinely applied to the rapid enumeration of E. coli in recreational waters and recommendations for its application were suggested to water quality managers. 相似文献
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If increased sediment supply to a river channel exceeds its transport capacity, deposition necessarily occurs as the bed adjusts to accommodate the increased supply. Both the mean and spatial patterns in bed elevation and grain size may change and an ability to understand their relative importance is needed to predict bed response. We report on an experiment in a field‐scale flume in which sediment supply is increased to a gravel bed with alternate bars. Sediment was recirculated in the experiments, but augmented in two steps, after which the bed was allowed to reach a new steady state. The transport rate at the end of the experiment was three times larger than at the start. High‐resolution sediment flux and topographic measurements, grain size derived from photographs, and hydrodynamic modeling allow us to document the topographic and textural response of the bed to increased sediment supply. The spatial patterns of bed topography and texture were forced by the flume setup and the initial and final steady states included long stationary alternate bars with associated grain size sorting. The transient bed contained several scales of shorter wavelength migrating bedforms superimposed on, and temporarily replacing the stationary alternate bars. Bed topography and textural patterns adjusted to increased sediment supply over different timescales. Bed slope and mean stress increased directly with sediment supply rate to produce a new transport steady state in a time about 2.5 times the minimum needed to deposit the required sediment wedge, indicating a trap efficiency of about 40% for the aggrading wedge. Adjustments in local topography and sorting, primarily in the form of smaller, migrating bars, continued for a period approximately equal to that required to initially reach transport steady state. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
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C. Martayan A. Alvarez P. Bristow R. Castillo H. Dekker S. D'Odorico V. Mainieri E. Mason A. Modigliani S. Moehler P. Moller F. Patat E. Pena C. Reinero J. Vernet J. Vinther M. Vuckovic all the engineers who work a lot on X‐shooter 《Astronomische Nachrichten》2011,332(3):222-223
We report on the current performances and measurements done with X‐shooter during its first year of operations at Paranal. We present the current instrumental problems, recent and future improvements, and give advice for the observations (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
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M. Pararó J. Pena R. Peniche C. Ibanoglu Z. Tunca S. Evren 《Astrophysics and Space Science》1993,210(1-2):185-187
Using unpublished data obtained in 1982 at Mexico and Turkey combined with Hungarian observations the process of amplitude variation of the frequency at 18.5 c/d is shown. The time scale of the amplitude increase is less than 100 days. 相似文献
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The stratigraphy of the western Portugal on-shore Cretaceous record (western Iberian margin, Lusitanian Basin) is described, including formal units and a selection of informal units prevailing in the geological literature. This paper is a synthesis based on a review of previous works, but with an innovative emphasis on the interpretation of eustatic and tectonic controls. The sedimentary record is dominated by siliciclastics and comprises fluvial and deltaic coastal marine siliciclastic systems, as well as extensive deposits of shallow marine carbonate platforms, both open and rimmed. Several regional unconformities and transgressive/regressive cycles are identified and the allogenic controls interpreted, namely the geodynamic events along the boundaries of the Iberian plate. Above the Berriasian deposits belonging to the Upper Jurassic cycle, the five main unconformity-bounded units are: (1) upper Berriasian–lower Barremian, (2) upper Barremian–lower Aptian, (3) upper Aptian–uppermost Cenomanian, (4) mid lower Turonian–lower Campanian and (5) middle Campanian–Maastrichtian. These units show transgressive peaks in the lower Hauterivian, lower Aptian, base of the upper Cenomanian and mid lower Turonian. The general trend of the Lower Cretaceous reflects the transition from late rifting to passive margin, with the last break-up unconformity dated as late Aptian. The Lusitanian Basin achieved full infill by the Cenomanian, when a large carbonate platform extended far inland. The later deposits were preserved only in the northern sector and the accompanying unconformities reflect transpressive intraplate stresses generated in boundaries of the plate with Africa and Eurasia. With very low accommodation being created throughout the Late Cretaceous, fluvial deposits were dominant, including a few marine levels related with eustatic rises in the early Turonian, the Coniacian, the early Campanian and the Maastrichtian. 相似文献
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L.D. Pena G. Francés P. Diz M. Esparza J.O. Grimalt M.A. Nombela I. Alejo 《Continental Shelf Research》2010
High resolution benthic foraminiferal stable isotopes (δ18O, δ13C) and molecular biomarkers in the sediments are used here to infer rapid climatic changes for the last 8200 years in the Ría de Muros (NW Iberian Margin). Benthic foraminiferal δ18O and δ13C potentially register migrations in the position of the hydrographic front formed between two different intermediate water masses: Eastern North Atlantic Central Water of subpolar origin (ENACWsp) and subtropical origin (ENACWst). The molecular biomarkers in the sediment show a strong coupling between continental organic matter inputs and negative δ13C values in benthic foraminifera. The rapid centennial and millennial events registered in these records have been compared with two well known North Atlantic Holocene records from the subtropical Atlantic sea surface temperatures (SST) anomalies off Cape Blanc, NW Africa and the subpolar Atlantic (Hematite Stained Grains percentage, subpolar North Atlantic). Comparison supports a strong link between high- and low-latitude climatic perturbations at centennial–millennial time scales during the Holocene. Spectral analyses also points to a pole-to-equator propagation of the so-called 1500 yr cycles. Our results demonstrate that during the Holocene, the NW Iberian Margin has undergone a series of rapid events which are likely triggered at high latitudes in the North Atlantic and are rapidly propagated towards lower latitudes. Conceivably, the propagation of these rapid climatic changes involves a shift in atmospheric and oceanic circulatory systems. 相似文献
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