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1.
Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 g/ml, but dropped off very sharply at higher protein concentrations. The apparent Km for 3-OH-BaP was 1.71 +/- 0.04 microM, and Vmax 1.26 +/- 0.16 nmol/min/mg protein (mean +/- SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4'-OH-CB72) and a model hexachloro-OH-PCB (4'-OH-CB159) were assayed with [14-C]-UDPGA and separation of the [14-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [14-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4'-OH-CB72 (Km 7.3 microM; Vmax 1.55 nmol/min/mg) than 4'-OH-CB159 (Km 16.1 microM; Vmax 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver. 相似文献
2.
ABSTRACTOil spill forecast modelling is typically used immediately after a spill to predict oil dispersal and promote mobilisation of more effective response operations. The aim of this work was to map oil dispersal after the grounding of the MV Rena on Astrolabe Reef and to verify the results against observations. Model predictions were broadly consistent with observed distribution of oil contamination. However, some hot spots of oil accumulation, likely due to surf-zone and rip current circulation, were not well represented. Additionally, the model was run with 81 differing wind conditions to show that the events occurring during the grounding represented the typical likely behaviour of an oil spill on Astrolabe Reef. Oil dispersal was highly dependent on prevailing wind patterns; more accurate prediction would require better observations of local wind patterns. However, comparison of predictions with observations indicated that the GNOME model was an effective low-cost approach. 相似文献
3.
In recent years, artificial establishment of Spartina alterniflora marshes has become a common method for mitigating impacts to salt marsh systems. The vegetative component of artificially established salt marshes has been examined in several studies, but relatively little is known about the other aspects of these systems. This study was undertaken to investigate the infaunal community of artificially established salt marshes. Infauna were sampled from pairs of artificially established (AE) salt marshes and nearby natural marshes at six sites along the North Carolina coast. The AE marshes ranged in age from 1 yr to 17 yr. Total infaunal density, density of dominant taxa, and community trophic structure (proportions of subsurface-deposit feeders, surface-deposit and suspension feeders, and carnivores) were compared between the two types of marsh to assess infaunal community development in AE marshes. Overall, the two marsh types had similar component organisms and proportions of trophic groups, but total density and densities within trophic groupings were lower in the AE marshes. Soil organic matter content of the natural marshes was nearly twice that of the AE marshes, and is a possible cause for the higher infaunal densities observed in the natural marshes, Using the same three criteria, comparisons of the natural and AE marshes at each of the six locations revealed varying degrees of similarity. Similarity of each AE marsh to its natural marsh control appeared to be influenced by differences in environmental factors between locations more than by AE marsh age. Functional infaunal habitat convergence of an AE marsh with a natural marsh somewhere within its biogeographical region is probable, but success in duplicating the infaunal community of a particular natural marsh is contingent upon the developmental age of the natural marsh and the presence and interaction, of site-specific factors. 相似文献
4.
Recently, detection of discrete features in the X-ray afterglow spectra of GRB 970508 and GRB 970828 was reported. The most natural interpretation of these features is that they are redshifted Fe K emission complexes. The identification of the line emission mechanism has drastic implications for the inferred mass of radiating material and hence the nature of the burst site. X-ray spectroscopy provides a direct observational constraint on these properties of gamma-ray bursters. We briefly discuss how these constraints arise in the context of an application to the spectrum of GRB 970508. 相似文献
5.
Various waterborne anthropogenic contaminants disrupt the endocrine systems of wildlife and humans, targeting reproductive pathways, among others. Very little is known, however, regarding the occurrence of endocrine disruptive activity in South African freshwater ecosystems, and coastal ecosystems have not been studied in this regard. In a first attempt to investigate endocrine disruptive activity in South African coastal waters, surface water samples collected from harbours, river mouths and estuaries in three metropolitan municipalities, eThekwini (which includes Durban), Nelson Mandela (specifically Port Elizabeth Harbour) and City of Cape Town, were screened for (anti) oestrogenicity and (anti)androgenicity using recombinant yeast bioassays. Moreover, levels of the female hormone 17β-(o)estradiol (E2) were determined in all samples, as well as a selection of hydrocarbons in the eThekwini samples. A high proportion of samples collected from eThekwini were oestrogenic, whereas none from Port Elizabeth Harbour and only a single river mouth sampled in the City of Cape Town were oestrogenic. E2 was detected in all the samples tested, but at higher concentrations at the eThekwini and City of Cape Town localities than Port Elizabeth Harbour. In addition, the recombinant yeast assays revealed that anti-androgenicity was widespread, being detected in the majority of samples screened apart from those representing Port Elizabeth Harbour. Conversely, no anti-oestrogenic or androgenic activity was detected. Anti-androgenicity did not associate with hydrocarbon loads, providing evidence that other anti-androgens were responsible for the observed activity. The present data suggest potential reproductive disruption in marine and estuarine fauna inhabiting the eThekwini and City of Cape Town regions. 相似文献
6.
Pier Luigi Sacco Maria Tartari Guido Ferilli Giorgio Tavano Blessi 《Urban geography》2019,40(4):529-554
In this paper, we critically examine the role of artistic locational choices and practices in the context of gentrification processes in urban renewal contexts. We characterize gentrification as a form of domestication of space, and explore the extent to which artistic choices and practices relate to such process with reference to the deontological dilemma of legitimization within the art system vs. responsible empowerment of vulnerable local constituencies. We illustrate our argument with an analysis of the High Line Art project, and show how this can be considered as a textbook example of art-driven space domestication leading to brutal forms of gentrification. We comment on the threat that this provides to the social credibility of artistic practices as an agency of responsible social change. 相似文献
7.
Rita Cannas Flavio Sacco Maria Cristina Follesa Andrea Sabatini Marco Arculeo Sabrina Lo Brutto Teresa Maggio Anna Maria Deiana Angelo Cau 《Marine Ecology》2012,33(3):350-363
Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (FST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex-biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource. 相似文献
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Predicting the fate of the injected CO2 is crucial for the safety of carbon storage operations in deep saline aquifers: especially the evolution of the position, the spreading and the quantity of the mobile CO2 plume during and after the injection has to be understood to prevent any loss of containment. Fluid flow modelling is challenging not only given the uncertainties on subsurface formation intrinsic properties (parameter uncertainty) but also on the modelling choices/assumptions for representing and numerically implementing the processes occurring when CO2 displaces the native brine (model uncertainty). Sensitivity analysis is needed to identify the group of factors which contributes the most to the uncertainties in the predictions. In this paper, we present an approach for assessing the importance of model and parameter uncertainties regarding post-injection trapping of mobile CO2. This approach includes the representation of input parameters, the choice of relevant simulation outputs, the assessment of the mobile plume evolution with a flow simulator and the importance ranking for input parameters. A variance-based sensitivity analysis is proposed, associated with the ACOSSO-like meta-modelling technique to tackle the issues linked with the computational burden posed by the use of long-running simulations and with the different types of uncertainties to be accounted for (model and parameter). The approach is tested on a potential site for CO2 storage in the Paris basin (France) representative of a project in preliminary stage of development. The approach provides physically sound outcomes despite the challenging context of the case study. In addition, these outcomes appear very helpful for prioritizing the future characterisation efforts and monitoring requirements, and for simplifying the modelling exercise. 相似文献
10.
McDonnell JA Alexander WM Burton WM Bussoletti E Evans GC Evans ST Firth JG Grard RJ Green SF Grun E Hanner MS Hughes DW Igenbergs E Kissel J Kuczera H Lindblad BA Langevin Y Mandeville JC Nappo S Pankiewicz GS Perry CH Schwehm GH Sekanina Z Stevenson TJ Zarnecki JC et AL 《Astronomy and Astrophysics》1987,187(1-2):719-741
Analysis of the data from Giotto's Dust Impact Detection System experiment (DIDSY) is presented. These data represent measurement of the size of dust grains incident on the Giotto dust shield along its trajectory through the coma of comet P/Halley on 1986 March 13/14. First detection occurred at some 287000 km distance from the nucleus on the inbound leg; the majority of the DIDSY subsystems remained operational after closest approach (604 km) yielding the last detection at about 202000 km from the nucleus. In order to improve the data coverage (and especially for the smallest grains, to approximately 10(-19) kg particle mass), data from the PIA instrument has been combined with DIDSY data. Flux profiles are presented for the various mass channels showing, to a first approximation, a 1/R2 flux dependence, where R is the distance of the detection point from the cometary nucleus, although significant differences are noted. Deviations from this dependence are observed, particularly close to the nucleus. From the flux profiles, mass and geometrical area distributions for the dust grains are derived for the trajectory through the coma. Groundbased CCD imaging of the dust continuum in the inner coma at the time of encounter is also used to derive the area of grains intercepted by Giotto. The results are consistent with the area functions derived by Giotto data and the low albedo of the grains deduced from infrared emission. For the close encounter period (-5 min to +5 min), the cumulative mass distribution function has been investigated, initially in 20 second periods; there is strong evidence from the data for a steepening of the index of the mass distribution for masses greater than 10(-13) kg during passage through dust jets which is not within the error limits of statistical uncertainty. The fluences for dust grains along the entire trajectory is calculated; it is found that extrapolation of the spectrum determined at intermediate masses (cumulative mass index alpha = 0.85) is not able to account for the spacecraft deceleration as observed by the Giotto Radio Science Experiment and by ESOC tracking operations. Data at large masses (>10(-8) kg) recently analysed from the DIDSY data set show clear evidence of a decrease in the mass distribution index at these masses within the coma, and it is shown that such a value of the mass index can provide sufficient mass for consistency with the observed deceleration. The total particulate mass output from the nucleus of comet P/Halley at the time of encounter would be dependent on the maximum mass emitted if this change in slope observed in the coma were also applicable to the emission from the nucleus; this matter is discussed in the text. The flux time profiles have been converted through a simple approach to modeling of the particle trajectories to yield an indication of nucleus surface activity. There is indication of an enhancement in flux at t approximately -29 s corresponding to crossing of the dawn terminator, but the flux detected prior to crossing of the dawn terminator is shown to be higher than predicted by simple modelling. Further enhancements corresponding to jet activity are detected around +190 s and +270 s. 相似文献