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1.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
HAN Jian LI Guoxiang Kubota Shin Ou Qiang Toshino Sho Wang Xing Yang Xiaoguang Uesugi Kentaro Masato Hoshino Sasaki Osamu Kano Harumas Sato Tomohiko Komiya Tsuyoshi 《《地质学报》英文版》2016,90(1):38-65
The early Cambrian pentamerous microfossil Olivooides/Punctatus in South China, which is characterized by a diagnostic stellate tubular apex, has been well-known for its almost complete development sequence that can be confidently traced from embryos and hatched juveniles, to conical adults. However, its zoological affinity remains highly controversial. Here we describe the internal microanatomic structures of the soft-body inside the peridermal theca of Olivooides multisulcatus Qian, 1977, including interradial pairs of tentacles, adradial and perradial frenula, perradial oral marginal lappets and twins of perradial gastric saccule-like humps as well as a circular velarium with striated coronal muscles. Particularly, one specimen shows bifurcated velarial canals along the bell aperture. Both the components of the soft-body and the external theca (or cyst) are arranged in perfect pentaradial symmetry. These characteristics are more compatible with those of living cubomedusans and co-occurring Cambrian athecate embryonic cubozoans. Concerning the presence of peridermal theca, Olivooides most likely represents an extinct thecate stem-group cubomedusae but devoid of both perradial eyes and specialized pedalia. The well-grown soft body inside the peridermal tube displays a set of mixed features of both polyp and medusa. 相似文献
3.
Naoki Niwa Takuji Kobori Motoichi Takahashi Tomohiko Hatada Haruhiko Kurino Jun Tagami 《地震工程与结构动力学》1995,24(5):655-671
This paper describes a feasibility study on a high-damping device (HiDAM) installed in a building structure by way of a bracing mechanism. A seismic response analysis with respect to a high-rise building approximately 100 m high is reported. Proper adjustment of the damping coefficient of the HiDAM (oil damper) provided an over all damping factor of about 10–20 per cent to the building structure, and reductions in the response, deformation and shear forces were verified analytically. This paper reports also the performance confirmation tests conducted on a model device of the HiDAM which satisfied the specifications determined from the analytical results. The test results demonstrate the feasibility of the HiDAM and the possibility of utilizing it in high-rise buildings. 相似文献
4.
The R/V Mirai conducted hydrographic surveys in the western Arctic Ocean during summer 2004 (Mirai04) over wide east-west
ranges from Alaska to eastern Siberia, where sea-ice cover has been greatly reduced in recent summers. The obtained data reveal
differences in silicate profiles between shelf slope areas east and west of the Chukchi Plateau, the ridge that divides the
Canada Basin into the Alaskan and east Siberian sides. East of the plateau, a single silicate maximum was found in a layer
of Pacific-origin winter water, as examined in many previous studies. In contrast, west of the plateau, we found vertical
double silicate maxima, which are reported for the first time in this study. The shallower silicate maximum corresponded to
an N** minimum, signaling denitrification at the shelf bottom. This suggests that the shallower silicate maximum was caused
by the spreading of shelf water. In contrast, the deeper silicate maximum corresponded to an oxygen minimum and a maximum
silicate/phosphate ratio (Si/P), suggesting that this deeper maximum resulted from the decomposition of opal-shelled organisms.
We also compared a silicate profile from Mirai04 to aprofile from the Arctic Ocean Section 94 (AOS94) expedition of 1994,
a heavy ice year. The results suggest that sea-ice loss has enhanced biological activities, likely resulting in the appearance
of the deeper silicate maximum. 相似文献
5.
Yoshida A Nomura H Toyoda K Nishino T Seo Y Yamada M Nishimura M Wada M Okamoto K Shibata A Takada H Kogure K Ohwada K 《Marine pollution bulletin》2006,52(1):89-95
Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups. 相似文献
6.
A numerical method has been developed for the dynamic analysis of a tall building structure with viscous dampers. Viscous dampers are installed between the top of an inverted V‐shaped brace and the upper beam on each storey to reduce vibrations during strong disturbances like earthquakes. Analytically, it is modelled as a multi‐degree‐of freedom (MDOF) system with the Maxwell models. First, the computational method is formulated in the time domain by introducing a finite element of the Maxwell model into the equation of motion in the discrete‐time system, which is based on the direct numerical integration. Next, analyses for numerical stability and accuracy of the proposed method are discussed. The results show its numerical stability. Finally, the proposed method is applied to the numerical analysis of a realistic building structure to demonstrate its practical validity. 相似文献
7.
Shin Takahashi Tomoko Oshihoi Tomohiko Isobe Tsunemi Kubodera 《Marine pollution bulletin》2010,60(2):187-196
Twelve species of deep-sea fishes collected in 2005 from the western North Pacific, off-Tohoku, Japan were analyzed for organohalogen compounds. Among the compounds analyzed, concentrations of DDTs and PCBs (up to 23,000 and 12,400 ng/g lipid wt, respectively) were the highest. The present study is the foremost to report the occurrence of brominated flame retardants such as PBDEs and HBCDs in deep-sea organisms from the North Pacific region. Significant positive correlations found between δ15N (‰) and PCBs, DDTs and PBDEs suggest the high biomagnification potential of these contaminants in food web. The large variation in δ13C (‰) values observed between the species indicate multiple sources of carbon in the food web and specific accumulation of hydrophobic organohalogen compounds in benthic dwelling carnivore species like snubnosed eel. The results obtained in this study highlight the usefulness of deep-sea fishes as sentinel species to monitor the deep-sea environment. 相似文献
8.
Malarvannan G Takahashi S Isobe T Kunisue T Sudaryanto A Miyagi T Nakamura M Yasumura S Tanabe S 《Marine pollution bulletin》2011,63(5-12):172-178
Three species of sea turtles (green, hawksbill and loggerhead turtles) stranded along the coasts or caught (by-catch) around Ishigaki Island and Kochi, Japan were collected between 1998 and 2006 and analyzed for six organohalogen compounds viz., PBDEs, PCBs, DDTs, CHLs, HCHs and HCB. The present study is the first and foremost to report the occurrence of organohalogen compounds in the sea turtles from Japan. Among the compounds analyzed, concentrations of PCBs, DDTs and CHLs were the highest in all the turtle samples. PBDEs were ubiquitously present in all the turtle species. Comparing with the other two species, concentrations of organohalogens in green turtle were relatively low and decreasing trend in the concentrations were noted with increasing carapace length. Concentrations of OCs in sea turtles from the coasts of Ishigaki Island and Kochi were relatively low as compared to those from other locations in the world. 相似文献
9.
10.
GIS-based weights-of-evidence modelling of rainfall-induced landslides in small catchments for landslide susceptibility mapping 总被引：10，自引：0，他引：10
Ranjan Kumar Dahal Shuichi Hasegawa Atsuko Nonomura Minoru Yamanaka Takuro Masuda Katsuhiro Nishino 《Environmental Geology》2008,54(2):311-324
Landslide susceptibility mapping is a vital tool for disaster management and planning development activities in mountainous terrains of tropical and subtropical environments. In this paper, the weights-of-evidence modelling was applied, within a geographical information system (GIS), to derive landslide susceptibility map of two small catchments of Shikoku, Japan. The objective of this paper is to evaluate the importance of weights-of-evidence modelling in the generation of landslide susceptibility maps in relatively small catchments having an area less than 4 sq km. For the study area in Moriyuki and Monnyu catchments, northeast Shikoku Island in west Japan, a data set was generated at scale 1:5,000. Relevant thematic maps representing various factors (e.g. slope, aspect, relief, flow accumulation, soil depth, soil type, land use and distance to road) that are related to landslide activity were generated using field data and GIS techniques. Both catchments have homogeneous geology and only consist of Cretaceous granitic rock. Thus, bedrock geology was not considered in data layering during GIS analysis. Success rates were also estimated to evaluate the accuracy of landslide susceptibility maps and the weights-of-evidence modelling was found useful in landslide susceptibility mapping of small catchments. 相似文献