排序方式： 共有2条查询结果，搜索用时 62 毫秒
1
1.
Development of a SCAR marker for male gametophyte of Gracilariopsis lemaneiformis based on AFLP technique 总被引：1，自引：0，他引：1
The red alga Gracilariopsis lemaneiformis （Bory） is an economically valuable macroalgae. As a means to identify the sex of immature Gracilariopsis lemaneiformis, the amplified fragment length polymorphism （AFLP） technique was used to search for possible sex- or phase-related markers in male gametophytes, female gametophytes, and tetrasporophytes, respectively. Seven AFLP selective amplification primers were used in this study. The primer combination E-TG/M-CCA detected a specific band linked to male gametophytes. The DNA fragment was recovered and a 402-bp fragment was sequenced. However, no DNA sequence match was found in public databases. Sequence characterized amplified region （SCAR） primers were designed from the sequence to test the repeatability of the relationship to the sex, using 69 male gametophytes, 139 female gametophytes, and 47 tetrasporophytes. The test results demonstrate a good linkage and repeatability of the SCAR marker to sex. The SCAR primers developed in this study could reduce the time required for sex identification of Gracilariopsis lemaneiformis by four to six months. This can reduce both the time investment and number of specimens required in breeding experiments. 相似文献
2.
以青岛湛山湾的单倍体和二倍体龙须菜以及青岛台湾路的单倍体和二倍体龙须菜为实验材料,采用AFLP技术,分析了不同地理群体内单倍体和二倍体种群以及不同世代种群间的遗传变异参数.湛山湾单倍体种群的Na(1.169 9)、Ne(1.095 0)、H(0.058 2)、I(0.088 4)与二倍体种群的Na(1.333 3)、Ne(1.227 0)、H(0.129 7)、I(0.190 8),以及台湾路单倍体种群的Na(1.137 3)、Ne(1.089 9)、H(0.050 4)、I(0.074 6)与二倍体种群的Na(1.209 2)、Ne(1.138 6)、H(0.082 5)、I(0.121 9)相比较,可以看出不同世代倍性的龙须菜的遗传多样性是有差别的,同一地理群体的二倍体在遗传变异上高于单倍体;而且湛山湾-台湾路单倍体种群间的Ht(0.058 1)、Gst(0.066 9)、Nm(6.968 3)和二倍体种群间Ht(0.179 9)、Gst(0.327 1)、Nm(1.028 5)的数值也有很大差别,单倍体和二倍体是处于不同分化层次的2个种群,根据Wright对Nm的研究可知,单倍体种群是中度分化的种群,二倍体种群是高度分化的种群.通过分析可知,龙须菜的遗传多样性水平较低,这为育种策略的选择提供了依据. 相似文献
1