EFFECT OF NITRATE AND PHOSPHATE ON ACCUMULATION OF β-CAROTENE ISOMERS IN DUNALIELLA SALINA     

刘建国  吴超元  陈念洪  王玉君  俞立东 《中国海洋湖沼学报》1996,(2)

DunaIiella salina, a halotolerent unicellular green alga, can accurmulate a Iarge amount of β-caroteneunder environmental conditions. The isorners of β-carotene extIacted from D. salina culturedin medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-Talumins column. At least six isomers were found in different proportions depending on the culture me-dia's nitrate and/or phosphate concentrations. Nitrate and/or phosphae defidency was conducive tothe accumulation of totaI cis isomers but not of al1 trans isomer. lt is sUggeSted that 1 mmol/L KNO_3and 0.1 mmol/L KH_2, PO_4 are favourable for accumulation of total cis β-carotene.  相似文献   

胁迫条件下盐藻β-胡萝卜素及其异构体累积的研究──盐度的影响   总被引：17，自引：0，他引：17  

刘建国  赵学武  王玉君  王作芸  陈念洪  俞立东  吴以平  吴超元 《海洋与湖沼》1994,25(1):71-76

于１９８９－１９９１年，在青岛室外对盐藻为实验材料，利用Ｊｏｈｎｓｏｎ改良配方制作增养液，进行盐度（９０－２４０）实验，结果表明，细胞生长的适宜盐度为１２０；当盐度超过１２０－２４０，盐度越高对细胞生长越不利，但越有利于细胞内β－胡罗卜素的累积；适宜于养殖盐藻生产β－胡萝卜素中至少含６种异构体，盐度增加不利于顺式异构体的累积。为生产防癌和延缓衰老等作用更强的顺式异构体，建议生产性养殖时适当降低盐度  相似文献