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1.
Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7,Fs, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9,9 loci for each primer, This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.321 2-0.476 7, and the maximum was between F7 and Fs (0.476 7). Identity analysis showed that F6 generation was more close to the female parent (0.659 3), and F7 generation was more close to the male parent (0.5788). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230-2 800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.223 9),female parent (0.107 2), F6 (0.216 4), F7 (0.228 6), F8 (0.229 6) and F9 (0.317 2). The nearest genetic distance was 0.083 5 (Fs, F9). Total heterozygosity (HT) ofF6, F7, F8 and F9 generations was 0.318 6, the average heterozygosity (Hs) for F6,F7, F8 and F9 generations was 0.248 0, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selecting Laminaria. 相似文献
2.
卡德藻自养、异养与兼养培养的比较研究 总被引：2，自引：0，他引：2
本文对卡德藻在自养、异养和兼养培养条件下的比生长速率、细胞密度、细胞色素组成及脂肪酸组成等方面进行了比较研究。兼养卡德藻的细胞密度大于自养条件和异养条件下细胞密度之和。兼养培养的卡德藻比生长速率是自养的 2倍 ,异养的 1.3倍。兼养和异养生长的对数期较自养的长。光合自养培养卡德藻最适光强为 10 0 0 0 lx,而兼养培养最适光强范围为 10 0 0～ 2 0 0 0 lx。在自养和兼养培养时亚油酸的含量很高 ,自养和兼养状态下分别为 16 .6 %和 17.4 9%。 EPA合成的量较低。异养藻的多不饱和脂肪酸的产量较自养藻和兼养藻要低。兼养藻细胞光合色素组成和含量与自养藻细胞的基本一致 ,但在异养条件下藻细胞色素组成发生明显改变。 相似文献
3.
遗传标记经历了形态标记、细胞学标记、蛋白质标记及DNA标记的四个重要发展历程。纵观遗传学的发展历史,遗传学的发展推动了新型遗传标记的发展,每一种新型遗传标记的发现,均推进了遗传理论与技术的发展。19世纪后期,孟德尔以豌豆为材料,利用7对形态学标记对杂种后代的不同个体依据性状表现进行归类分析,发现了著名的遗传分离规律和独立分配规律。细胞学标记是指能明确显示遗传多态性的细胞学特征,如染色体形态、数目和结构在不同物种中的差异等,可以作为一种遗传标记来进行基因定位。20世纪50年代,人们发现同工酶是一种可用于物种起源与进化研究、种质鉴定、分类等诸多领域的分子标记技术(Markert et al.,1959)20世纪后期以来,DNA分子标记技术不断涌现,相继建立了RFLP(restriction fragment lengthen polymorphisis)、DNA指纹 (DNA fingerprint)，RAPD (random anplified polymophism DNA), AFLP(amplified fragnent lengthen polynorphisn)、微卫星 DNA (microsate llite DNA).SNP(single nucleotide polymorphisn)等专门的技术，在生物研究中得以广泛应用。分子标记技术应用于藻类学研究较晚,主要用于藻类的系统发生、地理分布、种群遗传、分类、亲本鉴定、杂种优势的鉴别及种质鉴定等领域。 相似文献
4.
在普查影响中国降水量和气温的大气环流系统及其指数的基础上,选取高相关、独立性强的大气环流系统指数作为预测因子,采用过滤式的筛选因子方法,动态建立线性回归方法,构建区域月气候预测模型(Regional Monthly Climate Forecast Model,RMCFM),自主开发操作系统,设置人机交互界面,实现人机交流操作方式,并通过解释应用方法做出中国160个台站的月降水量和月气温预测。实践证明,RMCFM具备天气气候专家系统概念,具有运算速度快,结构清晰易操作等特点,预测准确率较高,应用RMCFM可提高区域气候预测能力。 相似文献
5.
小旋转椭球粒子群的微波散射特性 总被引：4，自引：1，他引：3
文中从不同方向线性偏振的入射波对小旋转椭球状降水粒子极化产生的散射出发 ,推导出散射能流密度函数 ,以及降水粒子群旋转轴处于不同状态下的散射截面 ,得出散射截面随降水粒子相态和入射波波长变化的一些曲线 ,其结果可供遥感反演计算使用。 相似文献
6.
Costaria costata (C. Agardh) Saunders is one of common kelps distributed in many coastal areas worldwide; however, in China, no reports have
been made on cultivation of the genus. To investigate potential cultivation of the species in the northern part of China,
trials on isolation and preservation of the gametophytes were conducted using C. costata from Korea; growth and development of the gametophytes were observed. We showed that at 10±1°C, 60 μmol m−2s−1 and 12:12 h (L:D), freshly released zoospores settled down within 1 hour, and then developed into the primary cell during
the following 2 days. After a vegetative growth phase lasting 6–8 days, female gametophytes became 3–4 times larger in diameter
than that of the primary cell, but still remained at a unicellular stage, while male gametophytes divided into 4–10 cells
with only a slight change in size. Fertilization occurred within 10 days after the zoospores were released from the sporangia,
and the apical and basal tissues of the juvenile sporophyte divided and differentiated into the blade and stipe. Temperature
and irradiance influenced gametophytic vegetative growth and developmental patterns. Generally, low irradiance (15 μmol m−2s−1 and 30 μmol m−2s−1) was unfavorable to the induction of fertility, but it enhanced female gametophyte division. The optimal conditions for vegetative
growth were 15°C and 30 μmol m−2s−1. After transplantation of the juvenile seedlings and after eight months cultivation, the harvested mature blade reached 194
cm in length and 32.7 cm in width. Our study proves that it is feasible to implement propagation and large scale cultivation
of C. costata in northern China. 相似文献
7.
XUAN Jinsong FENG Yanbin WENG Manli ZHAO Ge SHI Jinfeng YAO Jianting WANG Xiuliang GUO Baotai QIAO Lixian DUAN Delin WANG Bin 《海洋学报(英文版)》2012,31(6):139-148
A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2×10 5 pfu/ml.The average insert size of the cDNA library is about 1.6 kb.From the cDNA library,591 cDNA clones were randomly selected and sequenced.As a result,574 EST(expressed sequence tag) sequences were generated.All of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to CX943198.The cDNA library was screened with a α-32 p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra yezoensis.Four positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of L.japonica TPS cDNA sequence.After PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named LjTPS.LjTPS encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 Daltons.The LjTPS gene was successfully expressed in E.coli and rice.The LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance. 相似文献
8.
Since the 1980s, Sargassum fusiforme has been cultivated in Zhejiang, South China, and nowadays it becomes one of the important commercial seaweeds in China. With traditions of eating habits in the East Asian countries, this brown alga is used as food, because it contained functional oligo/polysaccharides and chemical components, and was regarded playing roles in antioxidant activities and regulating immunology. Through over 15 years' selection, breeding and cultivation, we obtained three strains with good traits and testified their characters during the production, which included the cultivars with high yield and other two good characters, either all the selected strains were applied in the Sargassum production. To avoid confusion during the selection and nursery, it was preferred to establish one fingerprint for distinguishing the Sargassum cultivars from different strains. Random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR) methods were adopted to analyze the genetic diversities of the selected S. fusiforme strains. With that, one fingerprint with RAPD markers was constructed, and one sequence characterized amplified region(SCAR) marker to S.fusiforme was obtained. It is indicated that the applied fingerprint could be valid in S. fusiforme genetic and germplasm justification, and will be positive to molecular marker assistance in its selection and cultivation. 相似文献
9.
陕北油田烃类微渗漏遥感化探特征 总被引：2，自引：0，他引：2
陕北油田油(气) 藏埋藏浅,易发生油气微渗漏,已发现烃类微渗漏与遥感、化探异常及温度场异常相关的实例。文中介绍了烃类微渗漏在遥感图像上的几种表现形式和近年来物化探、钻探验证结果,结果表明,遥感和物化探综合解译可以提高油气藏勘探成功率。 相似文献
10.
双线偏振雷达测雨公式的对比分析 总被引：4，自引：1，他引：3
以滴谱理论为基础,用模拟雨滴谱分布的方法,讨论了雨滴主普变化以及降水强度变化对双线偏振雷达测雨式的影响,分析了双线偏振雷达测雨式优于普通雷达的原因,进而对回归所得双线偏振雷达各测雨式的测雨精度进行了对比分析,提出子双线偏振雷达各测雨式中的最佳测雨式。 相似文献