Karlodinium elegans sp. nov. (Gymnodiniales,Dinophyceae), a novel species isolated from the East China Sea in a dinoflagellate bloom     

Cen  Jingyi  Wang  Jianyan  Huang  Lifen  Lin  Yarou  Ding  Guangmao  Qi  Yuzao  Lü  Songhui 《中国海洋湖沼学报》2021,39(1):242-258

Journal of Oceanology and Limnology - On May 24–29, 2019, a harmful algal bloom occurred in Pingtan coastal areas, Fujian, southeast China, and caused mass mortality of cage-cultured fish....  相似文献   

Karlodinium elegans sp. nov. (Gymnodiniales,Dinophyceae), a novel species isolated from the East China Sea in a dinofl agellate bloom     

Jingyi CEN  Jianyan WANG  Lifen HUANG  Yarou LIN  Guangmao DING  Yuzao QI  Songhui LÜ 《海洋湖沼学报（英文）》2021,(1):242-258

Abstract On May 24–29,2019,a harmful algal bloom occurred in Pingtan coastal areas,Fujian,southeast China,and caused mass mortality of cage-cultured fi sh.Two clonal cultures of an unknown naked dinofl agellate were set up from seawater samples taken during the bloom.The cultures were examined for morphological features,ultrastructure characters,photosynthetic pigments,the large subunit(LSU)of the rRNA gene,and the internal transcribed spacer(ITS)sequences,as well as acute toxicity analysis.The cell was unarmored,small-sized,and ovoid,and was characterized by elaborate striations on the epicone and hypocone.The nucleus was large,ellipsoid to oval or kidney-shaped,and centrally located in the cell.A long linear apical groove originated above the sulcus in the ventral epicone and extended to the dorsal side.An elongate,slit-like“ventral pore”was located on the left of the epicone,well away from the apical groove.The chloroplasts were yellowish brown,numerous,band like,and irregularly distributed in the cell periphery.Fucoxanthin was the main accessory pigment composition.Phylogeny topology reconstructed on partial LSU rDNA showed that the unknown dinofl agellate branched as a sister species to Karlodinium sp.(strain IFR981&IFR797,from France)and Karlodinium corrugatum(strain KDGSO08,from Australia),with genetic divergences of 0.6%and 3.3%,respectively.Based on the morphological and molecular phylogenetic analysis,we describe the novel dinofl agellate as Karlodinium elegans sp.nov.A toxicity assay revealed that the clonal culture of K.elegans(strain PTB601)had no adverse eff ect on brine shrimp(Artemia salina)and marine medaka(Oryzias melastigma),indicating it may not be a toxic species.  相似文献