排序方式： 共有2条查询结果，搜索用时 15 毫秒
1
1.
以一种分离自北极冰川融水的淡水微藻（Chlorella sp.）为实验材料,设置两个不同强度UV-B（45μw/cm2、76μw/cm2）照射处理、每4小时取样检测,对其生化组分进行分析,了解微藻对UV-B辐射的响应。研究结果显示小球藻经辐射处理后,其生化成分发生变化：（1）在较低强度的UV-B（45μw/cm2）辐射下,叶绿素a（Chla）和类胡萝卜素（Car）含量先升后降,且在4h时有最大值;高强度UV-B（76μw/cm2）辐射下,Chla和Car含量随辐射时间延长基本呈下降趋势。（2）超氧阴离子（O2?ˉ）和丙二醛（MDA）含量基本随辐照时间的延长和辐照强度的增大提高,过氧化氢（H2O2）含量变化虽随辐照时间的延长呈上升趋势,但直到12h是才表现为明显的提高。（3）超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性在低UV-B处理下,随辐照时间的延长呈升高趋势;在高UV-B辐射下,SOD活性先升后降,CAT活性上下波动。(4)UV-B辐射使小球藻脂肪酸组成发生了变化。这些结果表明：UV-B,尤其是高强度或较高剂量的UV-B会对北极冰川微藻带来不利的影响。 相似文献
2.
Standard FISH protocols using fluorochrome-labeled oligonucteotide probes have been successfully applied for in situ detection.However,optimized protocols of FISH for specific eukaryotes in marine environments are often not developed.This study optimized the conditions of fluorescence in situ hybridization (FISH) by using two polar isolated microalgae.The modified conditions were as follows: (1) 10 mg·mL-1 lysozyme solution pretreatment at 37℃ for 30 min;(2) the hybridization buffer including 20% formamide;(3) the hybridization condition was 47℃ for 6 h. The cells enumerated by FISH were compared with those enumerated by flow eytometry (FCM) and DAPI to confirm the cell loss and hybridization efficiency.The optimized protocol was also successfully applied to Arctic Ocean samples,which were found to be dominated by Micromonas sp.The modified protocol showed a high relative efficiency and could be successfully applied for the detection of specific microbial eukaryotes in environmental samples. 相似文献
1