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Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil 总被引：1，自引：0，他引：1
Mahalingam Brinda Lakshmi Venkataramagounder Perumal Anandaraj Manickam Velan 《洁净——土壤、空气、水》2013,41(1):86-93
The effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil–slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50–200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X‐100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X‐100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X‐100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites. 相似文献
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Climate change in the Mojave Desert (USA) may result in a greater intensity of summer (monsoon) rain events and greater atmospheric N deposition. Patches of the dominant biological crust moss Syntrichia caninervis were exposed to field treatments of low and high nitrogen supplementation, added summer rain, and combinations thereof, for a period of five years. In the lab, shoots were subjected to a moderate (single rapid-dry treatment) and a severe (double rapid-dry treatment) desiccation stress. Shoots were then hydrated and allowed to regenerate for 6 weeks on sterile sand. Shoots exhibited lower aboveground biomass from the +monsoon treatment, indicating carbon balance deficits under conditions of recurring summer rainfall. The low N treatment and the severe desiccation stress promoted shoot burning (chlorosis) and negatively affected regeneration responses. High N treatment, however, only adversely affected the resumption of apical meristematic growth. Summer irrigation and N application interacted to mitigate the effects of later protonemal emergence time, lower protonemal cover, and lower shoot numbers. Increased monsoonal frequency and higher N deposition as forecast for the northern Mojave Desert may result in declines in shoot biomass and reduced regenerational vigor in S. caninervis. 相似文献
3.
M. Beth Schlemper Brinda Athreya Kevin Czajkowski Victoria C. Stewart Sujata Shetty 《The Journal of geography》2019,118(1):21-34
Our project introduced students in grades 7 through 12 to spatial thinking and geospatial technologies in the context of challenges in their community. We used a mix of levels of inquiry to advance learning from teacher- to student-guided through a citizen mapping group activity. Student-suggested problem-based topics included parks and community gardens, crime, housing, and youth employment opportunities. Qualitative methods were used to evaluate students’ knowledge of spatial thinking and geospatial technologies, including map interpretation, a case study, daily exit slips, and interviews. Overall, the students’ awareness of their community, spatial thinking, and geospatial technologies increased as a result of participation. 相似文献
4.
We present and discuss in this paper the rest frame radio spectra (1–25 GHz) of a sample of fourteen radio galaxies atz >2 from the newly defined MRC/1Jy complete sample of 558 radio sources. These galaxies are among the most powerful radio sources
known and range in luminosity from 1028-1028·8 watt Hz-1 at 1 GHz. We find that the median rest frame spectral index of this
sample of galaxies atz >2 is significantly steeper than that of a matched luminosity sample of 3CRR galaxies which are at a much lower redshift (0.85
<z < 1.7). This indicates that spectral index correlates primarily with redshift, at least in the luminosity range considered
here. The difference between the distributions of rest frame spectral curvatures for the two samples does not appear to be
statistically significant.
We suggest a new explanation for the steeper spectra of radio galaxies at high redshift involving steeper electron energy
spectra at injection. Electron energy spectra are expected to steepen in a first-order Fermi acceleration process, at both
non-relativistic and relativistic shock fronts, as the upstream fluid velocity decreases. This may well be the case at high
redshifts: the hotter and denser circum-galactic medium at high redshifts could result in slower speeds for the hotspot and
the jet material behind it. The smaller sizes of radio sources at higher redshifts provide support to this scenario.
Since deceased. 相似文献
5.
This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed. 相似文献
6.
In the present work, experiments have been carried out with a focus to reduce the volume requirement of solvent by mixing with imidazolium based ionic liquids (ILs) for the solvent extraction of phenol, p‐chlorophenol, 2,4‐dichlorophenol, 2,4,6‐trichlorophenol, and pentachlorophenol from aqueous solutions. The effect of aqueous phase pH (2–12), agitation speed (100–450 rpm), solute concentration in feed (2–50 mg/L), temperature (303–333 K), treat ratio (1–11), and 1‐butyl‐3‐methyl imidazolium tetrafluoroborate [Bmim]+[BF4]? volume in tributyl phosphate (TBP; 0–0.7% v/v) on extraction of phenols has been studied and optimized. Parameters like strip phase pH (3–13) and stripping agent concentration (0.001–0.009 N) have also been studied for stripping of phenols from solvent phase. It has been found that 0.5% v/v of ionic liquid [Bmim]+[BF4]? in solvent TBP extracts more than 97.5% of phenol and chlorophenols from aqueous solutions with a treat ratio (aqueous to solvent phase ratio) of 5. Transport mechanism for extraction and stripping of phenol and chlorophenols using ionic liquid [Bmim]+[BF4]? has been discussed. The results show that by appropriate selection of extraction and stripping conditions, it is possible to remove nearly all phenols with a treat ratio of 5. 相似文献
7.
This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. 相似文献
8.
Veeresh Singh Prajval Shastri Ramana Athreya 《Journal of Astrophysics and Astronomy》2011,32(4):497-500
Seyfert galaxies are classified mainly into type 1 and type 2 depending on the presence and absence of broad permitted emission
lines in their optical spectra, respectively. Unification scheme hypothesizes that the observed similarities and differences
between the two Seyfert subtypes can be understood as due to the differing orientations of anisotropically distributed obscuring
material having a torus-like geometry around the AGN. We investigate the radio continuum emission properties of a sample of
Seyfert galaxies in the framework of the unification scheme. 相似文献
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