Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

A STUDY ON LAND SYSTEM INNOVATIONS IN THE CONSTRUCTION OF SMALL TOWNS IN CHINA     

LIANGLin－ke  CHANGJiang 《中国地理科学(英文版)》2002,12(1):80-85

The economic development in China and the rising of the living standard need to speed up the urbanisa-tion.The development of small towns is an important way to Chinese urbanization.Land use plays a very important role in the development of small towns.However there are many problems in the development of small towns,esp.in land use.The paper first discusses the land problems in the development of small towns.Such as much cultivated land lies idle,under-utilization and waste of land,increasing illegal use of land,unstable contractural relationship for land use.The relationship between the development of small towns and land use is also studied.Then the guidelines for the land system innovations of small towns are put forward.Namely the sustainability of social and economic development,the bal-ance between land reservation and land utilization,the provision of service to village ,agriculture and farmer,the manage-ment of land resource and land assets,the parsimonious andlegal use of land.The basic framework of land system innova-tions of small towns is put forward finally.It include the land replacement policy for small towns and the permanent tenan-cy of farmland,the overall plan for land uses and other plans in harmony,the establishment of a flexible system of land supplies,using land with payments ,the transfer of agricultural land and refining the land law related to the construction of small towns.  相似文献   

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）     

Juei-Hwa Hu  Wing-Huen Ip 《中国天文和天体物理学报》2005,5(4):356-362

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.  相似文献   

Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes     

Zhang Shicui  Wu Xianhan  Zhou Jing  Wang Renxue  Wu Shangqin 《中国海洋湖沼学报》1990,8(3):263-272

Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down. Contribution No. 1722 from the Institute of Oceanology, Academia Sinica  相似文献   

Evolution of Electric Currents Associated with Two M-Class Flares     

Baolin Tan  Haisheng Ji  Guangli Huang  Tuanhui Zhou  Qiwu Song  Yu Huang 《Solar physics》2006,239(1-2):137-148

Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare.  相似文献   

The formation and stability of the configuration of the planetary system HD 126611     

Zhang Xu-dong  Zhou Ji-lin 《Chinese Astronomy and Astrophysics》2006,30(4):420-430

Recent Doppler velocity measurements have revealed the existence of two planets orbiting the star HD 12661 on medium-eccentricity orbits. The inner planet has a period of 263.6 d and a mass of 2.3 Jupiter masses, and the outer planet has a period of 1444.5d and a mass of 1.57 Jupiter masses. The stability of this system requires the two planets to be in a state of mean-motion orbit resonances. By numerical method we have studied the orbit migration and stability of the system in its early ages under the action of the proto-stellar disk, and calculated the probabilities of the planets being captured into the mean -motion resonances during their migrations. It is found that at present the two planets are probably situated at the edge of the 11:2 mean-motion resonance and are in chaotic motions. This result may be helpful to clarify the arguments on the present configuration. Besides, it is indicated that very probably, after the formation of the system, the gaseous disk has almost disappeared before the planets migrated to the present configuration.  相似文献   

Optimal Filtering Algorithm for Stochastic 2-D FMM Ⅱ with Multiplicative Noise     

CHUDongsheng LIANGMeng SHIXin ZHANGLing 《中国海洋大学学报(英文版)》2004,3(1):106-110

1　Introduction　Inrecentyearstherehasbeengrowingresearchin terestinthetwo dimensional (2 D )systemtheory .The 2 Dsystemsmayfindapplicationsinareassuchasmarineseismicdataprocessingandimageprocessing .Althoughmoreandmorevaluableresultshavebeengained ,mos…  相似文献   

浙西北江山─绍兴断裂带构造演化特征     

竺国强  姜继双  陈梓军  周培华 《浙江国土资源》1997,(2)

江山—绍兴断裂带是中国南方最重要的构造带之一。它是江南陆块与华夏陆块神功运动形成的碰撞拼贴带。它表现出明显的多期变形、构造叠加的特征：神功运动形成近东西向平卧褶皱；印支运动沿断裂带有向北西强烈的推覆挤压。断裂带不同时期的活动反映了不同特征的构造环境，形成不同的构造变形特征，并对两侧的地质历史具有明显的控制作用。  相似文献   

台湾海峡西岸重力场变化梯度场图像分析     

梁全强  刘序俨  黄声明  吴维日  陈新泽 《大地测量与地球动力学》2006,26(4):82-85

讨论了应用曲面拟合技术和利用离散的重力观测点的重力变化对特定研究区的重力场变化进行数值模拟以获取该区重力变化空间梯度场图像的具体方法;并应用该方法获得了2004年3月福建安溪3.6级地震前的重力变化空间梯度场图像,发现空间梯度场图像能清晰地反映出地震前重力场群体性变化的差异性;利用此方法对2005年6月~2006年3月间台湾海峡西岸重力场变化进行数值模拟,获得其空间梯度场图像。  相似文献   

Re-Identification of the ＇Enigmatic＇ X-ray Source 1RXS J114003.0＋124112     

Jiang-Hua Wu Tigran Movsessian Yang Chen Xiang-Tao He Xu Zhou Jun Ma 《中国天文和天体物理学报》2006,6(4):430-434

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