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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
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Hengchang Dai 《Geophysical Prospecting》2003,51(4):347-364
Shear waves recorded at or near the sea-bed, i.e. a water–sediment interface, may suffer from unwanted phase change, which is detrimental to velocity analysis and processing and degrades the quality of the final stacked or migrated image. In this study, this phenomenon is analysed for P-SV converted waves recorded at the sea-bed. Theoretical analysis shows that phase change does not occur if the converted shear waves always maintain raypaths that lie within the critical angle, provided the subsurface layering is horizontal. A phase change that is asymmetric with offset can readily be explained as being due to dipping layers at targets or the dipping sea-bed. This analysis is extended to multiple layers and anisotropic media and shows that the same conclusions hold. The analysis performed on two sets of ocean-bottom-cable seismic data shows that the majority of observations show little evidence of phase change, and occasionally display the asymmetric phase change with offset. This finding underlines the robustness of converted shear waves for imaging the horizontal subsurfaces and sea-bed as all of the offset information may be used. 相似文献
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Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed. 相似文献
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应用地壳波浪与镶嵌构造学说对富氏谱分析法提取地壳垂直形变信息的科学性做了地质学意义上的阐释 ,并提出了根据多期形变资料提取特定波段上构造策应力的数学模型 相似文献
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Yi-Zhong Fan Zi-Gao Dai Yong-Feng Huang Tan Lu Department of Astronomy Nanjing University Nanjing 《中国天文和天体物理学报》2002,2(5):449-453
The optical flash accompanying GRB 990123 is believed to be powered by the reverse shock of a thin shell. With the best-fit physical parameters for GRB 990123 and the assumption that the parameters in the optical flash are the same as in the afterglow, we show that: 1) the shell is thick rather than thin, and we have provided the light curve for the thick shell case which coincides with the observation; 2) the theoretical peak flux of the optical flash accounts for only 3×10~-4 of the observed. In order to remove this discrepancy, the physical parameters, the electron energy and magnetic ratios, εe and εB, should be 0.61 and 0.39, which are very different from their values for the late afterglow. 相似文献
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We have used Monte Carlo simulations to investigate the capabilities of a giant air shower observatory designed to detect showers initiated by cosmic rays with energies exceeding 1019 eV. The observatory is to consist of an array of detectors that will characterise the air shower at ground level, and optical detectors to measure the fluorescence light emitted by the shower in the atmosphere. Using these detectors together in a ‘hybrid’ configuration, we find that precise geometrical reconstruction of the shower axis is possible, leading to excellent resolution in energy. The technique is also shown to provide very good reconstruction below 1019 eV, at energies where the ground array is not fully efficient. 相似文献