Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

河外H2O超脉泽源及与其相关的分子谱线的观测和研究     

俞志尧 《天文学进展》2002,20(3):256-264

自从在活动星系核NGC4945的视线方向上发现第一个河外H2O超脉泽源以来，迄今为止已发现了19个河外H2O超脉泽源，对与活动星系核成协和河外H2O超脉泽源及分子谱线的观测和研究是探测和研究活动星系核核区中央源，拱核气体和尘埃环性质的非常有效的工具，主要评述对河外H2O超脉泽源及与其相关分子谱线的搜索，观测和理论研究现状。  相似文献   

BORDER PORT MANZHOULh URBAN FUNCTION AND SPACE DEVELOPMENT     

ZHANGPing-yu MAYan-ji YUZhen-han 《中国地理科学(英文版)》2002,12(4):315-320

Manzhouli is the largest land port city on the Sino-Russia border, transit cargo through the land port amount-ed to 5.95 million tons, transit tourists were 304 500 in 2000. It stands at the joint place of China, Mongolia and Russi-a, faces to Siberia area of Russia, receives direct support from the Northeast China and Bohai Sea Rim Area, and possess-es priorities in geographical location, land port infrastructure, water resources, coal resources, tourist resources andgreat potentiality in economic cooperation with Russia. The future urban function is a key port on the First Eurasia Continen-tal Bridge. Manzhouli Port will keep its first place between China and Russia land transport, and it is forecasted that thetransit amount through Manzhouli Port will go up to 10 million tons in 2005 and 20 million tons in 2010. It will be construct-ed to be a trade center of the peripheral area extending to Russia and Mongolia, a key export-oriented processing industri-al zone supported by industries such as export processing industries, export agriculture, trade services, technology trad-ing and the other service industries. It keeps being a well-known touring city for trade, shopping, sightseeing, vocation,local food, recreation and cultural events. To build Manzhouli Export Processing Industry Zone will improve city econom-ic structure, and the main sectors are organic food processing, livestock products processing, garment and furniture indus-try. Moreover, Manzhouli Export Processing Industry Zone will eventually be upgraded to be a border free trade zone.The city functional transition will inevitably affect urban spatial restructure and its expansion. The city space transforma-tion will develop as such: one development axis of No. 301 highway paralleling with Bin - Zhou(Harbin - Manzhouli) rail-way which cuts through central part of Manzhouli City, and links Zhalainuocr District with central city; three urban unitsincluding central city, Zhalainuocr District and Manzhouli Interchange Trade Zone; cohesion with Aoerjin and Cuogangpastures; regional dual-nuclei structure of Hailaer City and Manhzouli City; and the Manzhouli-Zabaykalsk Free TradeZone.  相似文献   

青藏高原隆升的非线性动态有限元仿真研究   总被引：7，自引：4，他引：3  

杨红心  王乘  张秋文  郑文衡 《大地测量与地球动力学》2002,22(2):75-81

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。  相似文献   

SPATIAL—TEMPORAL VARIATION OF HEAVY METAL ELEMENTS CONTENT IN COVERING SPIL OF RECLAMATION AREA IN FUSHUN COAL MINE     

YUJun－bao  LIUJing－shuang 《中国地理科学(英文版)》2002,12(3):268-272

Grid method is employed for sampling covering soil at the test field,which is reclamation area filled by coal mining wastes for cropland in the Fushun coal mine,Liaoning Province,the Northeast China.The soil samples are taken at different locations,including three kinds of covering soil,three different depths of soil layers and four different covering ages of covering soil.The spatial-temporal variation of heavy metal element content in reclamation soil is stud-ied.The results indicate that the content of heavy metal elements is decreasing year after year,the determinant reason why the content of heavy metal elements at 60cm depth layer is higher than that at 30cm depth layer and surface is fertiliz-er and manure application;the metal elements mainly come from external environment;there is no metal pollution coming from mother material (coal mining wastes)in plough layer of covering soil.  相似文献   

大同—阳高Ms6.1地震前兆综合加权信息熵的变化     

张昭栋  刘章增  赵会蓬 《大地测量与地球动力学》2002,22(1):51-55

利用“地震预报计算机专家系统”的思想对大同 -阳高 Ms6 .1地震前每一前兆异常事件进行综合评估 ,以每一异常的最可能发震时间来计算发震概率 ,利用地震前兆综合加权信息熵研究了系统熵值与地震的关系。对华北地区的地震前兆综合加权信息熵研究表明 ,在大同 -阳高 Ms6 .1地震前 ,信息熵出现了明显的减熵有序变化  相似文献   

对青藏东北缘现今块体划分、运动及变形的初步研究   总被引：19，自引：9，他引：10  

张晓亮  江在森  陈兵  李辉 《大地测量与地球动力学》2002,22(1):63-67

利用2维非连续变形分析方法（DDA），以位移代替围压作为边界约束力，研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动，以GPS点测量位移作为模拟结果约束点，得出了较合理的块体划分模型和随时间演化的主应变分布图，并把应变高值区与近几年来发生的5级以上地震作对比，得出了研究区内地震危险性可能较大的区域。另外，对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。  相似文献   

抽油机悬点投影测量仪     

文志雄  戚克军  李明  曾南志 《大地测量与地球动力学》2002,22(1):110-113

油田抽油机的抽油杆幌动幅度过大，是引起抽油杆断裂的一个重要因素，检测这种幌动幅度是防止抽油杆断裂的一种有效手段。设计了一种基于面阵CCD和普通半导体激光器（LD）测量这种幌动的悬点投影测量方法，通过数字卷积滤波，达到了范围为0－40mm，误差＜0.2mm的技术检测指标。  相似文献   

Discovery of 13 New Variable Stars in the Field of the Open Cluster NGC 2168 （M35）     

Juei-Hwa Hu  Wing-Huen Ip 《中国天文和天体物理学报》2005,5(4):356-362

A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars.  相似文献   

Influence of the Choice of Core-Envelope Transition Point on the Binary Merger of Two Main-sequence Components     

Xue-Fei Chen  Zhan-Wen HanNational Astronomical Observatories  Yunnan Observatory  Chinese Academy of Sciences. Kunming  Graduate School of the Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2005,5(1):65-76

We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the  相似文献