Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

银盘恒星运动学和化学丰度与银河系结构     

侯金良  陈力  常瑞香  孙振娜 《天文研究与技术》2004,1(1):16-27

大样本的银盘恒星的运动学数据和元素丰度特征是研究银河系结构及演化，尤其是银盘形成和演化的2个重要探针。本文首先介绍了银河系的总体结构特征，然后较详细的综述了以上2个探针的重要性。对我国LAMOST项目在研究银河系结构方面，尤其是银盘的形成和演化方面的能力作了合理的分析。  相似文献   

观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

  相似文献   

Geochemical survey of thermal system in Northeast China and their geothermometric characters     

MohammedHazaea HUKe MohammedMattash CHENBing M.O.AI-JabaliAref MohammedAbdussalam 《东北亚地学研究》2004,7(2):181-184

The chemical composition of several thermal springs around Changbaishan area has been investigated. Cenozoic basaltic rocks are widely distributed in Northeast China and geothermal characteristics have been described. About one hundred hot springs exist around Changhaishan Volcano at the border between China and D.P.R.Korea with high temperature about 82~C. The pH values of the spring water range from 6.9 to 7.1 and the total flow rate is about 4.8 L/sec. The chemical composition of the thermal springs is sodium carbonate; the high-mineral contents of thermal water are believed to have medicinal properties. Bathhouses are already built along the hot springs to take the advantage of the supposed healing properties. The high quality of those hot springs is believed to be utilitized for mineral water. The chemical equilibrium temperatures were estimated at about 160℃ based on the Na-K-Ca geochemical thermometer.  相似文献   

隧道工程地质评价的内容和方法   总被引：2，自引：0，他引：2  

陈新建  孙琳  李新星  孙巧银 《地球科学与环境学报》2004,26(1):41-45

结合工程隧道实际,首先阐述了隧道工程的基本地质环境和工程地质条件,然后针对隧道工程可能出现的不良地质现象和可采取的工程措施,从大气降水、围岩稳定、围岩压力、洞口稳定、隧道比选等角度探讨隧道工程地质评价的主要方法和一般内容,进行隧道工程地质评价,为隧道施工、支护提供了依据.  相似文献   

MATLAB在运用系统建模处理南海气象数据过程中应用     

董言治  周晓东  娄树理  陈永刚  沈同圣 《成都信息工程学院学报》2004,19(1):76-81

介绍了MATLAB语言特点和系统建模方法的基本理论.根据南海气象数据的实际建模处理过程,给出了建模的详细步骤及其MATLAB实现过程以及MATLABTM的主要实现程序.试验讨论和结果表明利用MATLAB语言可以方便地对南海气象数据用系统建模方法进行建模和处理,MATLAB在运用系统建模法处理南海气象数据方面具有明显的优越性.  相似文献   

影响鸢乌贼手钓上钩率的因子探讨     

吴国峰  陈新军 《广东海洋大学学报》2006,26(6):35-38

根据印度洋西北部鸢乌贼（Sym plectoteuthis oualaniensis）作业渔场的现场调查数据．分析了作业水深、作业时段、摄食等级和月相等因子对手钓上钩率的影响。统计表明．晚上、午夜和凌晨3个不同时段和不同作业水深对手钓上钩率均有显著性影响。3个时段中，其摄食等级呈先低后高再低的现象。午夜和凌展，上钩率的变化趋势与摄食等级的变化趋势呈显著的负相关。在月相的影响下．离朔日三四天之前，手钓产量呈上升趋势；离望日五六天之前。手钓产量呈明显的下降趋势。  相似文献   

Microwave Type III Burst Group in the 1993 October 2 Flare     

Qijun Fu  Zhihai Qin  Yuying Liu  Guangli Huang 《Astrophysics and Space Science》1997,250(2):189-204

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.  相似文献   

A diagnostic study on heat sources and moisture sinks in the monsoon trough area during active-break phases of the Indian summer monsoon, 1979     

U. V. BHIDE  V. R. MUJAMDAR  S. P. GHANEKAR  D. K. PAUL  TSING-CHANG CHEN  G. V. RAO 《地球，A辑:动力气象学与海洋学》1997,49(4):455-473

  相似文献