A novel method for evaluating proneness of gas outburst based on gas-geological complexity     

Wei  Guo-Ying  Kang  Fang-Chao  Qin  Bin-Bin  Jia  Tian-Rang  Yan  Jiang-Wei  Feng  Zhen-Dong 《Natural Hazards》2020,104(2):1841-1858

The gas outburst, resulting in substantial economic losses and even casualties, is the biggest obstacle in coal mines, mostly caused by an imbalance of gas-geological structure. For accurately measuring this proneness, in this paper, a novel evaluation method was proposed based on the gas-geology theory. In this method, a standardization model of statistical units was presented first, which was used to standardize and quantify the 12 chosen gas-geological factors; and then, an associated function was established for computing the gas-geological complexity index (GCI). With increasing GCI values, the evaluated area was divided into four grades: simple, medium, complex, and extremely complex region, in which the associated proneness of outbursts was SAFE, POTENTIAL, HIGH, STRONG, respectively. Taking the XueHu Coal Mine as an example, site verification was carried out with a good result. Research and application indicate that (1) gas outburst is unbalanced and closely related to the complex of the gas geological structure, showing a greater GCI leads to a higher outburst possibility; (2) the most likely area for the gas outburst is the extremely complex region and the transition zone between adjacent areas with different GCI grades; (3) upgrading-targeted control measures are the best way for preventing and controlling disasters caused by the gas and outburst unbalanced distribution. This novel method provided a reliable quantity approach for predicting and zonally managing gas outbursts and improving the effectiveness of outbursts prevention.

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应用LAMP-LFD技术可视化检测河流弧菌(Vibrio fluvialis)的研究   总被引：2，自引：0，他引：2  

杨梦香  柴方超  周前进  苗亮  黄光亮  陈炯 《海洋与湖沼》2017,48(2):383-391

以河流弧菌(Vibrio fluvialis)为检测对象,将环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)与横向流动试纸条(lateral flow dipstick,LFD)联合应用,建立了快速便捷的LAMP-LFD方法。该方法以河流弧菌的外膜蛋白omp U基因作为检测靶标,在其保守区域设计6条特异性引物(上游内引物由生物素标记),并在两条外引物的有效扩增区段内设计1条特异性探针,由异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记。使用引物进行有生物素标记的LAMP反应,产物与FITC标记的探针完成杂交,杂交产物在LFD上进行结果展示。结果表明,LAMP-LFD方法可特异性地检出河流弧菌,对近似物种如创伤弧菌等弧菌病原以及迟缓爱德华菌等其他水产养殖病原的检测均呈阴性。经优化,LAMP的反应条件为63°C反应25min,加上5min的探针杂交和3—5min的LFD显色,整个检测时程约35min。利用该方法最低可检测到1.0×10~2 CFU/m L的河流弧菌纯培养物,能够从污染强度为5.0×10~2 CFU/m L的组织样品中检测到该病原。该方法设备依赖性更低,仅需简单的恒温加热设备即可完成。因此,本研究建立的河流弧菌LAMP-LFD方法,具有操作便捷,设备依赖性低、灵敏度高和检测快速等优点,在河流弧菌的基层检测中具有良好的应用前景。  相似文献