Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

Simulation experiment on hydrocarbon generating potential of various source rocks on the northern margin of the Qaidam Basin,Qinghai, China     

Xin Li  Dongliang Wang  Baoquan Liu  Chunling Ren  Jianying Guo  Xuefeng Su  Jian Wang 《中国地球化学学报》2008,27(4):412-419

The northern margin of the Qaidam Basin is one of the main oil-gas exploration areas in western China, where source rocks are composed mostly of Middle and Lower Jurassic dark mud shale, carbargillite and coal. A large number of subsurface and outcrop samples differing in lithology with different types of organic matter were selected for resource evaluation, research and calculation. And among them, 13 samples were used for simulation experiment on hydrocarbon generating potential of various source rocks. At first, two kinds of heating modes were compared through simulation experiment, including single temperature-step heating and continual heating. Perhaps, the process of hydrocarbon generation and expulsion occurred naturally between a close system and an open system. In addition, the first heating mode was convenient, and all its reaction products were involved in the whole thermal evolution, and the final simulation experimental results were basically in consistency with the natural evolution trend Therefore, the first heating mode was adopted and the hydrocarbon yield of every sample was worked out. According to the type and lithology of organic matter and the hydrocarbon yield of samples for simulation experiment, hydrocarbon generation and expulsion mode with three kinds of lithology and five types of source rock has been established to provide the basis for hydrocarbon generation evaluation, research and resource calculation.  相似文献   

基于Flash技术的综合电子地图集制作技术   总被引：1，自引：1，他引：0  

陈春玲  刘小栋 《地理空间信息》2008,6(4):126-128

重点介绍了基于Flash平台制作多媒体综合电子地图集的开发集成方法以及具体功能的实现。  相似文献   

GIS技术支持下的招远市金矿区崩塌遥感调查     

吴泉源  姜春玲  邹敏  杨圣军  张祖陆 《山地学报》2006,24(6):672-677

在总结前人研究成果的基础上,全面系统地分析了诱发招远市金矿区崩塌的自然和人为因数,然后运用遥感技术对金矿区遥感图像进行处理,提取诱发崩塌的条件因子,聘请有经验的专家对各项诱发因子进行诊断分析,以此作为判断条件,在G IS技术的支持下对诱发崩塌的条件因子进行空间分析,预测出招远金矿区发生崩塌的危害程度,为防治崩塌提供科学依据。预测结果表明,招远金矿区崩塌有进一步发展的可能,其中,高危险区有36820 m2,中易发区有50 610 m2,低易发区有67 200 m2,需要采取有效措施加以防治。  相似文献   

试论工程项目投资控制   总被引：1，自引：0，他引：1  

王春玲 《中国煤田地质》2003,15(5):68-69

工程项目投资控制是工程项目管理的重要内容。如何减少投资，并最大限度地提高企业投资收益，已为广大房地产开发商及项目管理者热切关注，即为热点又是难点。长期以来，人们总是把投资控制放在审查施工单位的进度款和决算方面，然而这种控制存在明显的缺陷。因为在施工过程中，经常发现那些因设计不科学、不合理脱离实际而造成的投资浪费，且又无法得到纠正的情况，从而使工程投资控制限于十分被动的局面。  相似文献   

辽西义县组奔龙类化石一新种   总被引：4，自引：1，他引：4  

刘金远  姬书安唐烽　高春玲 《地质通报》2004,23(8):778-783

在辽宁西部义县头台的义县组中新发现的1件奔龙类化石标本，显示出许多中国鸟龙属(Sinornithosaurus)所具有的特征，如上颌骨眶前窝侧面具有装饰性凹坑、前颌骨齿无边缘小锯齿、齿骨后缘二分叉、指节Ⅲ-1略长于指节Ⅲ-2的2倍、踱骨Ⅲ部分窄踱型形态等。但其头骨的部分骨骼与千禧中国鸟龙(S．millenii)有明显的区别：前颌骨主体部分相对较高，其长仅稍大于其高，前颌骨角大，前颌骨上颌突与鼻突均很长，上颌骨不参与外鼻孔的构成，上颌骨窗相对较小且为圆形，方颧骨上升突明显长于颧骨突，齿骨长高之比小等。此外，新标本肠骨的耻骨柄前后方向的宽度小于髋臼宽度。这些不同之处表明，义县头台的标本应为中国鸟龙属另一新种，将其命名为郝氏中国鸟龙(新种)(S．haoiana sp．nov.)。  相似文献   

地下水水动力弥散问题中弥散度的单孔确定方法研究     

王运国  毕春玲 《吉林地质》1991,10(2):50-60

本文提出了利用单孔进行弥散实验,确定地下水含水层弥散度的新方法,给出了数学模型、求解过程及方法。提出了用计算机自动处理求得最佳参数的直线回归法与非线性迭代法相结合的方法。不但进行了理论上的详细推导,而且通过实例进行了验证,结果很好。本文提出的方法原理通俗易懂,计算过程简单,易于实行,成本低,具有重要实际意义。  相似文献   

辽西朝阳地区下白垩统九佛堂组一新的离龙类   总被引：1，自引：0，他引：1  

高春玲  吕君昌  刘金远  季强 《地质论评》2005,51(6):694-697,i0003

根据发现于辽宁省西部朝阳地区下白垩统九佛堂组的离龙类一具有头骨的不完整骨架，建立Simoedosauridae科中的一新属新种：朝阳辽西龙：Liaoxisaurus chaoyangensis gen．etsp．nov．。朝阳辽西龙的下颌缝合部短，小于下颌长度的20％，牙齿齿槽近似正方形，区别于任何已知的进步的离龙类。它吻部相对较短，占头骨总长的49．8％。  相似文献   

地市级区域地图集的选题策划与设计思路     

陈春玲 《地理空间信息》2007,5(5):103-105

地图集在经济建设中起着越来越重要的作用,区域地图集已成为国际上科学编图的新趋势。结合地市级区域特点,探讨了图集设计过程中的内容组织及选题思想。  相似文献   

喜马拉雅山东南地区地质灾害发育规律初步研究   总被引：2，自引：0，他引：2  

童立强  祁生文  刘春玲 《工程地质学报》2007,15(6):721-729

利用遥感手段,结合MapGis,研究了喜马拉雅山东南地区地质灾害的发育情况,发现本区发育的主要地质灾害有滑坡、崩塌、泥石流、冰湖以及堰塞湖。其中崩塌、滑坡、泥石流斜坡地质灾害是本区最重要的地质灾害类型,占到总灾害数量的95.3%。在此基础上对喜马拉雅山东南地区地质灾害发育规律初步研究,发现本区地质灾害的发育在空间上的分布并非均匀,而是具有丛集性的特点。滑坡灾害主要发育在隆子和朗县。泥石流灾害比较严重的有米林、隆子和洛扎3县,而崩塌则主要集中在隆子县。研究发现,本区滑坡发育与地层、地形坡度以及土地类型关系密切,其中修康群、日当组和念青唐古拉群是本区的易滑地层。涅如组由于面积大,其中发育的滑坡较多,但是滑坡的发育率只略高于本区的平均水平。统计表明,16～30的坡度范围是滑坡最容易发生的。大于45以上的坡段很少发生滑坡。灌木林和天然草地这两种土地类型滑坡发育率最高。对于泥石流,研究表明,涅如组中泥石流发育面积最大,发育率也最高。泥石流发育的最适宜坡度也是16～30这样一个坡度范围。冰川和永久积雪区则最易发生泥石流。崩塌发育与地层类型、坡度的关系较为密切,崩塌主要发育在涅如组中,并且集中在坡度大于60以上的陡坡段中。这些初步成果的取得,是以后进行该区地质灾害空间预测的基础。  相似文献